Combining morphological and genomic evidence to resolve species diversity and study speciation processes of the Pallenopsis patagonica (Pycnogonida) species complex

• Published in: Frontiers in Zoology
• Main Authors: Dömel, Jana S., Macher, Till-Hendrik, Dietz, Lars, Duncan, Sabrina, Mayer, Christoph, Rozenberg, Andrey, Wolcott, Katherine, Leese, Florian, Melzer, Roland R.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2019
• Subjects: ddc:57; ddc:570; Fakultät für Biologie » Aquatische Ökologie; Forschungszentren » Zentrum für Wasser- und Umweltforschung (ZWU); Sea spider; Marine benthos; Antarctica; Patagonia; Integrative taxonomy; Target hybrid enrichment; Cryptic species; Selection; Antarctic; Hoek; Antarc*
• Online Access: https://doi.org/10.1186/s12983-019-0316-y; https://nbn-resolving.org/urn:nbn:de:hbz:464-20190910-140106-9; https://duepublico2.uni-due.de/receive/duepublico_mods_00049250; https://duepublico2.uni-due.de/servlets/MCRFileNodeServlet/duepublico_derivate_00048041/Doemel_et_al_Combining_morphological.pdf

Background: Pallenopsis patagonica (Hoek, 1881) is a morphologically and genetically variable sea spider species whose taxonomic classification is challenging. Currently, it is considered as a species complex including several genetic lineages, many of which have not been formally described as species. Members of this species complex occur on the Patagonian and Antarctic continental shelves as well as around sub-Antarctic islands. These habitats have been strongly influenced by historical large-scale glaciations and previous studies suggested that communities were limited to very few refugia during glacial maxima. Therefore, allopatric speciation in these independent refugia is regarded as a common mechanism leading to high biodiversity of marine benthic taxa in the high-latitude Southern Hemisphere. However, other mechanisms such as ecological speciation have rarely been considered or tested. Therefore, we conducted an integrative morphological and genetic study on the P. patagonica species complex to i) resolve species diversity using a target hybrid enrichment approach to obtain multiple genomic markers, ii) find morphological characters and analyze morphometric measurements to distinguish species, and iii) investigate the speciation processes that led to multiple lineages within the species complex. Results: Phylogenomic results support most of the previously reported lineages within the P. patagonica species complex and morphological data show that several lineages are distinct species with diagnostic characters. Two lineages are proposed as new species, P. aulaeturcarum sp. nov. Dömel & Melzer, 2019 and P. obstaculumsuperavit sp. nov. Dömel, 2019, respectively. However, not all lineages could be distinguished morphologically and thus likely represent cryptic species that can only be identified with genetic tools. Further, morphometric data of 135 measurements showed a high amount of variability within and between species without clear support of adaptive divergence in sympatry. Conclusions: We ...