Metabolic profile and genotoxicity in obese rats exposed to cigarette smoke
Objective Experimental studies have shown that exposure to cigarette smoke has negative effects on lipid metabolism and oxidative stress status. Cigarette smoke exposure in nonpregnant and pregnant rats causes significant genotoxicity (DNA damage). However, no previous studies have directly evaluate...
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ftunivespir:oai:repositorio.unesp.br:11449/76096 2023-07-02T03:33:36+02:00 Metabolic profile and genotoxicity in obese rats exposed to cigarette smoke Damasceno, Débora Cristina Sinzato, Yuri K. Bueno, Aline Dallaqua, Bruna Lima, Paula H. Calderon, Iracema de Mattos Paranhos Rudge, Marilza Vieira Cunha Campos, Kleber Eduardo de Universidade Estadual Paulista (UNESP) 2013-08-01 1596-1601 http://hdl.handle.net/11449/76096 https://doi.org/10.1002/oby.20152 eng eng Obesity 4.042 2,060 http://dx.doi.org/10.1002/oby.20152 Obesity, v. 21, n. 8, p. 1596-1601, 2013. 1930-7381 1930-739X http://hdl.handle.net/11449/76096 doi:10.1002/oby.20152 WOS:000323521500014 2-s2.0-84883140839 0679387622604743 6758680388835078 orcid:0000-0002-9227-832X closedAccess cholesterol fatty acid glutamate sodium glutathione peroxidase high density lipoprotein cholesterol low density lipoprotein cholesterol superoxide dismutase thiobarbituric acid reactive substance triacylglycerol very low density lipoprotein cholesterol animal experiment animal model blood sampling cholesterol blood level controlled study DNA damage enzyme activity fatty acid blood level female glucose intolerance lipid metabolism nonhuman obesity oxidative stress protein expression protein function rat smoking triacylglycerol blood level Nicotiana tabacum Rattus Rattus norvegicus info:eu-repo/semantics/article 2013 ftunivespir https://doi.org/10.1002/oby.20152 2023-06-12T16:19:05Z Objective Experimental studies have shown that exposure to cigarette smoke has negative effects on lipid metabolism and oxidative stress status. Cigarette smoke exposure in nonpregnant and pregnant rats causes significant genotoxicity (DNA damage). However, no previous studies have directly evaluated the effects of obesity or the association between obesity and cigarette smoke exposure on genotoxicity. Therefore, the aim of the present investigation was to evaluate DNA damage levels, oxidative stress status and lipid profiles in obese Wistar rats exposed to cigarette smoke. Design and Methods Female rats subcutaneously (sc) received a monosodium glutamate solution or vehicle (control) during the neonatal period to induce obesity. The rats were randomly distributed into three experimental groups: control, obese exposed to filtered air, and obese exposed to tobacco cigarette smoke. After a 2-month exposure period, the rats were anesthetized and killed to obtain blood samples for genotoxicity, lipid profile, and oxidative stress status analyses. Results The obese rats exposed to tobacco cigarette smoke presented higher DNA damage, triglycerides, total cholesterol, free fatty acids, VLDL-c, HDL-c, and LDL-c levels compared to control and obese rats exposed to filtered air. Both obese groups showed reduced SOD activity. These results showed that cigarette smoke enhanced the effects of obesity. Conclusion In conclusion, the association between obesity and cigarette smoke exposure exacerbated the genotoxicity, negatively impacted the biochemical profile and antioxidant defenses and caused early glucose intolerance. Thus, the changes caused by cigarette smoke exposure can trigger the earlier onset of metabolic disorders associated with obesity, such as diabetes and metabolic syndrome. Copyright © 2012 The Obesity Society. Article in Journal/Newspaper Rattus rattus Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP Obesity 21 8 1596 1601 |
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Open Polar |
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Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
op_collection_id |
ftunivespir |
language |
English |
topic |
cholesterol fatty acid glutamate sodium glutathione peroxidase high density lipoprotein cholesterol low density lipoprotein cholesterol superoxide dismutase thiobarbituric acid reactive substance triacylglycerol very low density lipoprotein cholesterol animal experiment animal model blood sampling cholesterol blood level controlled study DNA damage enzyme activity fatty acid blood level female glucose intolerance lipid metabolism nonhuman obesity oxidative stress protein expression protein function rat smoking triacylglycerol blood level Nicotiana tabacum Rattus Rattus norvegicus |
spellingShingle |
cholesterol fatty acid glutamate sodium glutathione peroxidase high density lipoprotein cholesterol low density lipoprotein cholesterol superoxide dismutase thiobarbituric acid reactive substance triacylglycerol very low density lipoprotein cholesterol animal experiment animal model blood sampling cholesterol blood level controlled study DNA damage enzyme activity fatty acid blood level female glucose intolerance lipid metabolism nonhuman obesity oxidative stress protein expression protein function rat smoking triacylglycerol blood level Nicotiana tabacum Rattus Rattus norvegicus Damasceno, Débora Cristina Sinzato, Yuri K. Bueno, Aline Dallaqua, Bruna Lima, Paula H. Calderon, Iracema de Mattos Paranhos Rudge, Marilza Vieira Cunha Campos, Kleber Eduardo de Metabolic profile and genotoxicity in obese rats exposed to cigarette smoke |
topic_facet |
cholesterol fatty acid glutamate sodium glutathione peroxidase high density lipoprotein cholesterol low density lipoprotein cholesterol superoxide dismutase thiobarbituric acid reactive substance triacylglycerol very low density lipoprotein cholesterol animal experiment animal model blood sampling cholesterol blood level controlled study DNA damage enzyme activity fatty acid blood level female glucose intolerance lipid metabolism nonhuman obesity oxidative stress protein expression protein function rat smoking triacylglycerol blood level Nicotiana tabacum Rattus Rattus norvegicus |
description |
Objective Experimental studies have shown that exposure to cigarette smoke has negative effects on lipid metabolism and oxidative stress status. Cigarette smoke exposure in nonpregnant and pregnant rats causes significant genotoxicity (DNA damage). However, no previous studies have directly evaluated the effects of obesity or the association between obesity and cigarette smoke exposure on genotoxicity. Therefore, the aim of the present investigation was to evaluate DNA damage levels, oxidative stress status and lipid profiles in obese Wistar rats exposed to cigarette smoke. Design and Methods Female rats subcutaneously (sc) received a monosodium glutamate solution or vehicle (control) during the neonatal period to induce obesity. The rats were randomly distributed into three experimental groups: control, obese exposed to filtered air, and obese exposed to tobacco cigarette smoke. After a 2-month exposure period, the rats were anesthetized and killed to obtain blood samples for genotoxicity, lipid profile, and oxidative stress status analyses. Results The obese rats exposed to tobacco cigarette smoke presented higher DNA damage, triglycerides, total cholesterol, free fatty acids, VLDL-c, HDL-c, and LDL-c levels compared to control and obese rats exposed to filtered air. Both obese groups showed reduced SOD activity. These results showed that cigarette smoke enhanced the effects of obesity. Conclusion In conclusion, the association between obesity and cigarette smoke exposure exacerbated the genotoxicity, negatively impacted the biochemical profile and antioxidant defenses and caused early glucose intolerance. Thus, the changes caused by cigarette smoke exposure can trigger the earlier onset of metabolic disorders associated with obesity, such as diabetes and metabolic syndrome. Copyright © 2012 The Obesity Society. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Article in Journal/Newspaper |
author |
Damasceno, Débora Cristina Sinzato, Yuri K. Bueno, Aline Dallaqua, Bruna Lima, Paula H. Calderon, Iracema de Mattos Paranhos Rudge, Marilza Vieira Cunha Campos, Kleber Eduardo de |
author_facet |
Damasceno, Débora Cristina Sinzato, Yuri K. Bueno, Aline Dallaqua, Bruna Lima, Paula H. Calderon, Iracema de Mattos Paranhos Rudge, Marilza Vieira Cunha Campos, Kleber Eduardo de |
author_sort |
Damasceno, Débora Cristina |
title |
Metabolic profile and genotoxicity in obese rats exposed to cigarette smoke |
title_short |
Metabolic profile and genotoxicity in obese rats exposed to cigarette smoke |
title_full |
Metabolic profile and genotoxicity in obese rats exposed to cigarette smoke |
title_fullStr |
Metabolic profile and genotoxicity in obese rats exposed to cigarette smoke |
title_full_unstemmed |
Metabolic profile and genotoxicity in obese rats exposed to cigarette smoke |
title_sort |
metabolic profile and genotoxicity in obese rats exposed to cigarette smoke |
publishDate |
2013 |
url |
http://hdl.handle.net/11449/76096 https://doi.org/10.1002/oby.20152 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
Obesity 4.042 2,060 http://dx.doi.org/10.1002/oby.20152 Obesity, v. 21, n. 8, p. 1596-1601, 2013. 1930-7381 1930-739X http://hdl.handle.net/11449/76096 doi:10.1002/oby.20152 WOS:000323521500014 2-s2.0-84883140839 0679387622604743 6758680388835078 orcid:0000-0002-9227-832X |
op_rights |
closedAccess |
op_doi |
https://doi.org/10.1002/oby.20152 |
container_title |
Obesity |
container_volume |
21 |
container_issue |
8 |
container_start_page |
1596 |
op_container_end_page |
1601 |
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1770273621530378240 |