Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Processo FAPESP: 2018/0458-6 CNPq: 423583/2018-2 CAPES: 001 CNPq: PQ 302713/2018-2 CNPq: PQ 301766/20...
Published in: | Aquatic Toxicology |
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Elsevier B.V.
2021
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Online Access: | http://hdl.handle.net/11449/209811 https://doi.org/10.1016/j.aquatox.2020.105717 |
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ftunivespir:oai:repositorio.unesp.br:11449/209811 2023-07-02T03:33:21+02:00 Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol Sousa, Gabriela Tavares Lima Neto, Milton C. Choueri, Rodrigo Brasil Castro, Italo Braga Universidade Estadual Paulista (UNESP) 2021-01-01 9 http://hdl.handle.net/11449/209811 https://doi.org/10.1016/j.aquatox.2020.105717 eng eng Elsevier B.V. Aquatic Toxicology http://dx.doi.org/10.1016/j.aquatox.2020.105717 Aquatic Toxicology. Amsterdam: Elsevier, v. 230, 9 p., 2021. 0166-445X http://hdl.handle.net/11449/209811 doi:10.1016/j.aquatox.2020.105717 WOS:000600837200025 Antioxidant metabolism Photosynthesis Triazine NPQ Ocean acidification info:eu-repo/semantics/article 2021 ftunivespir https://doi.org/10.1016/j.aquatox.2020.105717 2023-06-12T17:27:50Z Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Processo FAPESP: 2018/0458-6 CNPq: 423583/2018-2 CAPES: 001 CNPq: PQ 302713/2018-2 CNPq: PQ 301766/2019-3 Anthropogenic changes such as ocean acidification, eutrophication, and the release of hazardous chemicals affect coastal environments and aquatic organisms. We investigated the effects of seawater pH (7.4 and 8.2) isolated and in combination with Irgarol on Ulva lactuca. Stress indicators such as membrane damage, lipid peroxidation, and hydrogen peroxide content were assessed. In addition, chlorophyll fluorescence and antioxidant enzyme activities were measured. The photosynthetic yield was affected by low pH in assays with and without Irgarol. However, the combination of low pH and Irgarol promoted photoinhibition, besides the induction of nonphotochemical quenching (NPQ) and changes in photosynthetic pigment contents. The induction of NPQ was directly influenced by low pH. The membrane damage was increased in low pH with and without Irgarol exposure. Total soluble protein and carbohydrate contents decreased in low pH, and in presence of Irgarol. The H2O2 content and lipid pemxidation were not affected by low pH. In contrast, Irgaml exposure strongly increased lipid peroxidation in both pHs, suggesting a possible synergistic effect. To avoid the harmful effects of high H2O2, U. lactuca increased antioxidant enzyme activities in treatments under low pH and in presence of Irgaml. Our results indicate that U. lactuca is tolerant to low pH by inducing NPQ, changing pigment contents, and increasing antioxidant defenses. In contrast, these protective mechanisms could not avoid the harmful effects of the combination with Irgaml. Article in Journal/Newspaper Ocean acidification Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP Aquatic Toxicology 230 105717 |
institution |
Open Polar |
collection |
Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
op_collection_id |
ftunivespir |
language |
English |
topic |
Antioxidant metabolism Photosynthesis Triazine NPQ Ocean acidification |
spellingShingle |
Antioxidant metabolism Photosynthesis Triazine NPQ Ocean acidification Sousa, Gabriela Tavares Lima Neto, Milton C. Choueri, Rodrigo Brasil Castro, Italo Braga Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
topic_facet |
Antioxidant metabolism Photosynthesis Triazine NPQ Ocean acidification |
description |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Processo FAPESP: 2018/0458-6 CNPq: 423583/2018-2 CAPES: 001 CNPq: PQ 302713/2018-2 CNPq: PQ 301766/2019-3 Anthropogenic changes such as ocean acidification, eutrophication, and the release of hazardous chemicals affect coastal environments and aquatic organisms. We investigated the effects of seawater pH (7.4 and 8.2) isolated and in combination with Irgarol on Ulva lactuca. Stress indicators such as membrane damage, lipid peroxidation, and hydrogen peroxide content were assessed. In addition, chlorophyll fluorescence and antioxidant enzyme activities were measured. The photosynthetic yield was affected by low pH in assays with and without Irgarol. However, the combination of low pH and Irgarol promoted photoinhibition, besides the induction of nonphotochemical quenching (NPQ) and changes in photosynthetic pigment contents. The induction of NPQ was directly influenced by low pH. The membrane damage was increased in low pH with and without Irgarol exposure. Total soluble protein and carbohydrate contents decreased in low pH, and in presence of Irgarol. The H2O2 content and lipid pemxidation were not affected by low pH. In contrast, Irgaml exposure strongly increased lipid peroxidation in both pHs, suggesting a possible synergistic effect. To avoid the harmful effects of high H2O2, U. lactuca increased antioxidant enzyme activities in treatments under low pH and in presence of Irgaml. Our results indicate that U. lactuca is tolerant to low pH by inducing NPQ, changing pigment contents, and increasing antioxidant defenses. In contrast, these protective mechanisms could not avoid the harmful effects of the combination with Irgaml. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Article in Journal/Newspaper |
author |
Sousa, Gabriela Tavares Lima Neto, Milton C. Choueri, Rodrigo Brasil Castro, Italo Braga |
author_facet |
Sousa, Gabriela Tavares Lima Neto, Milton C. Choueri, Rodrigo Brasil Castro, Italo Braga |
author_sort |
Sousa, Gabriela Tavares |
title |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
title_short |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
title_full |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
title_fullStr |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
title_full_unstemmed |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
title_sort |
photoprotection and antioxidative metabolism in ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of irgarol |
publisher |
Elsevier B.V. |
publishDate |
2021 |
url |
http://hdl.handle.net/11449/209811 https://doi.org/10.1016/j.aquatox.2020.105717 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Aquatic Toxicology http://dx.doi.org/10.1016/j.aquatox.2020.105717 Aquatic Toxicology. Amsterdam: Elsevier, v. 230, 9 p., 2021. 0166-445X http://hdl.handle.net/11449/209811 doi:10.1016/j.aquatox.2020.105717 WOS:000600837200025 |
op_doi |
https://doi.org/10.1016/j.aquatox.2020.105717 |
container_title |
Aquatic Toxicology |
container_volume |
230 |
container_start_page |
105717 |
_version_ |
1770273254321160192 |