Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment
L-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects....
Published in: | Preparative Biochemistry & Biotechnology |
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Online Access: | http://hdl.handle.net/11449/202103 https://doi.org/10.1080/10826068.2020.1815053 |
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ftunivespir:oai:repositorio.unesp.br:11449/202103 2023-07-02T03:29:47+02:00 Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment Freire, Rominne Karla Barros Mendonça, Carlos Miguel Nóbrega Ferraro, Rafael Bertelli Moguel, Ignacio Sánchez Tonso, Aldo Lourenço, Felipe Rebello Santos, João Henrique Picado Madalena Sette, Lara Durães Pessoa Junior, Adalberto Universidade Estadual Paulista (UNESP) 2020-01-01 http://hdl.handle.net/11449/202103 https://doi.org/10.1080/10826068.2020.1815053 eng eng Preparative Biochemistry and Biotechnology http://dx.doi.org/10.1080/10826068.2020.1815053 Preparative Biochemistry and Biotechnology. 1532-2297 1082-6068 http://hdl.handle.net/11449/202103 doi:10.1080/10826068.2020.1815053 2-s2.0-85090947334 Cold-adapted yeast enzyme production glutaminase-free asparaginase L-asparaginase Leucosporidium muscorum info:eu-repo/semantics/article 2020 ftunivespir https://doi.org/10.1080/10826068.2020.1815053 2023-06-12T17:23:27Z L-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects. Among 134 yeast strains isolated from marine-sediments in King George Island, Antarctica, nine were L-asparaginase producing yeasts and glutaminase-free. Leucosporidium muscorum CRM 1648 yielded the highest ASNase activity (490.41 U.L−1) and volumetric productivity (5.12 U.L−1 h−1). Sucrose, yeast extract and proline were the best carbon and nitrogen sources to support growth and ASNase production. A full factorial design analysis pointed the optimum media condition for yeast growth and ASNase yield: 20 g L−1 sucrose, 15 g L−1 yeast extract and 20 g L−1 proline, which resulted in 4582.5 U L−1 and 63.64 U L−1 h−1 of ASNase and volumetric productivity, respectively. Analysis of temperature, pH, inoculum and addition of seawater indicated the best condition for ASNase production by this yeast: 12–15 °C, pH 5.5–6.5 and seawater >25% (v/v). Inoculum concentration seems not to interfere. This work is pioneer on the production of ASNase by cold-adapted yeasts, highlighting the potential of these microbial resources as a source of glutaminase-free L-asparaginase for commercial purposes. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP Antarctic King George Island Preparative Biochemistry & Biotechnology 51 3 277 288 |
institution |
Open Polar |
collection |
Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
op_collection_id |
ftunivespir |
language |
English |
topic |
Cold-adapted yeast enzyme production glutaminase-free asparaginase L-asparaginase Leucosporidium muscorum |
spellingShingle |
Cold-adapted yeast enzyme production glutaminase-free asparaginase L-asparaginase Leucosporidium muscorum Freire, Rominne Karla Barros Mendonça, Carlos Miguel Nóbrega Ferraro, Rafael Bertelli Moguel, Ignacio Sánchez Tonso, Aldo Lourenço, Felipe Rebello Santos, João Henrique Picado Madalena Sette, Lara Durães Pessoa Junior, Adalberto Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
topic_facet |
Cold-adapted yeast enzyme production glutaminase-free asparaginase L-asparaginase Leucosporidium muscorum |
description |
L-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects. Among 134 yeast strains isolated from marine-sediments in King George Island, Antarctica, nine were L-asparaginase producing yeasts and glutaminase-free. Leucosporidium muscorum CRM 1648 yielded the highest ASNase activity (490.41 U.L−1) and volumetric productivity (5.12 U.L−1 h−1). Sucrose, yeast extract and proline were the best carbon and nitrogen sources to support growth and ASNase production. A full factorial design analysis pointed the optimum media condition for yeast growth and ASNase yield: 20 g L−1 sucrose, 15 g L−1 yeast extract and 20 g L−1 proline, which resulted in 4582.5 U L−1 and 63.64 U L−1 h−1 of ASNase and volumetric productivity, respectively. Analysis of temperature, pH, inoculum and addition of seawater indicated the best condition for ASNase production by this yeast: 12–15 °C, pH 5.5–6.5 and seawater >25% (v/v). Inoculum concentration seems not to interfere. This work is pioneer on the production of ASNase by cold-adapted yeasts, highlighting the potential of these microbial resources as a source of glutaminase-free L-asparaginase for commercial purposes. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Article in Journal/Newspaper |
author |
Freire, Rominne Karla Barros Mendonça, Carlos Miguel Nóbrega Ferraro, Rafael Bertelli Moguel, Ignacio Sánchez Tonso, Aldo Lourenço, Felipe Rebello Santos, João Henrique Picado Madalena Sette, Lara Durães Pessoa Junior, Adalberto |
author_facet |
Freire, Rominne Karla Barros Mendonça, Carlos Miguel Nóbrega Ferraro, Rafael Bertelli Moguel, Ignacio Sánchez Tonso, Aldo Lourenço, Felipe Rebello Santos, João Henrique Picado Madalena Sette, Lara Durães Pessoa Junior, Adalberto |
author_sort |
Freire, Rominne Karla Barros |
title |
Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
title_short |
Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
title_full |
Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
title_fullStr |
Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
title_full_unstemmed |
Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
title_sort |
glutaminase-free l-asparaginase production by leucosporidium muscorum isolated from antarctic marine-sediment |
publishDate |
2020 |
url |
http://hdl.handle.net/11449/202103 https://doi.org/10.1080/10826068.2020.1815053 |
geographic |
Antarctic King George Island |
geographic_facet |
Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_relation |
Preparative Biochemistry and Biotechnology http://dx.doi.org/10.1080/10826068.2020.1815053 Preparative Biochemistry and Biotechnology. 1532-2297 1082-6068 http://hdl.handle.net/11449/202103 doi:10.1080/10826068.2020.1815053 2-s2.0-85090947334 |
op_doi |
https://doi.org/10.1080/10826068.2020.1815053 |
container_title |
Preparative Biochemistry & Biotechnology |
container_volume |
51 |
container_issue |
3 |
container_start_page |
277 |
op_container_end_page |
288 |
_version_ |
1770272611017687040 |