Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Processo FAPESP: 2017/03552-5 Processo FAPESP: 2016/06931-4 CNPq: 407588/2013-2 Leishmaniasis is cons...
Published in: | Journal of Applied Phycology |
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Online Access: | http://hdl.handle.net/11449/185605 https://doi.org/10.1007/s10811-018-1592-1 |
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ftunivespir:oai:repositorio.unesp.br:11449/185605 2023-07-02T03:29:53+02:00 Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) Rangel, Karen C. Debonsi, Hosana M. Clementino, Leandro C. Graminha, Marcia A. S. Vilela, Leonardo Z. Colepicolo, Pio Gaspar, Lorena R. Universidade Estadual Paulista (UNESP) 2019-04-01 825-834 http://hdl.handle.net/11449/185605 https://doi.org/10.1007/s10811-018-1592-1 eng eng Springer Journal Of Applied Phycology http://dx.doi.org/10.1007/s10811-018-1592-1 Journal Of Applied Phycology. Dordrecht: Springer, v. 31, n. 2, p. 825-834, 2019. 0921-8971 http://hdl.handle.net/11449/185605 doi:10.1007/s10811-018-1592-1 WOS:000463988500002 openAccess Cytotoxicity Leishmanicidal Leishmania amazonensis GC-MS analysis Iridaea cordata Rhodophyta info:eu-repo/semantics/article 2019 ftunivespir https://doi.org/10.1007/s10811-018-1592-1 2023-06-12T17:14:07Z Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Processo FAPESP: 2017/03552-5 Processo FAPESP: 2016/06931-4 CNPq: 407588/2013-2 Leishmaniasis is considered a neglected disease and affects billions around the world, currently presenting few therapeutic options, which makes the development of new antileishmanial drugs urgent. Secondary metabolites from marine and terrestrial organisms are important sources of new chemical entities. Herein, the potential activity of crude extracts and fractions from the Antarctic macroalga Iridaea cordata (Turner) Bory de Saint-Vincent was studied against promastigote and intracellular amastigotes forms of Leishmania amazonensis. The cytotoxicity of the active fractions was evaluated on macrophages and 3T3 BALB/c fibroblasts. The chemical profile of volatile substances was analyzed using the GC-MS technique. The fractions IC-FE (IC50-AMA = 23.6 +/- 3.4 mu g mL(-1); SI > 11) and IC-FF (IC50-AMA = 12.4 +/- 1.2 mu g mL(-1); SI > 24) showed promising activity against amastigotes and higher selectivity to the parasite rather than to the mammalian host cells, when compared to the reference drug amphotericin B (IC50-AMA = 5.9 +/- 0.3 mu g mL(-1); SI = 3.9). Their estimated LD50 in rodents are also higher than that in amphotericin B (LD50 IC-FE = 594.5 +/- 25.87 mg kg(-1); LD50 IC-FE = 580.1 +/- 11.84 mg kg(-1); LD50 amphoter = 172.20 +/- 2.40 mg kg(-1)). The chemical profile of these fractions showed the presence of phthalates, esters, ketones, fatty acids, and carboxylic acids, which might be contributing alone or synergistically to the observed antileishmanial activity. Consequently, I. cordata might be used to identify new antileishmanial compounds. Article in Journal/Newspaper Antarc* Antarctic Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP Antarctic Bory ENVELOPE(40.372,40.372,64.489,64.489) Saint-Vincent ENVELOPE(-59.515,-59.515,50.600,50.600) The Antarctic Journal of Applied Phycology 31 2 825 834 |
institution |
Open Polar |
collection |
Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
op_collection_id |
ftunivespir |
language |
English |
topic |
Cytotoxicity Leishmanicidal Leishmania amazonensis GC-MS analysis Iridaea cordata Rhodophyta |
spellingShingle |
Cytotoxicity Leishmanicidal Leishmania amazonensis GC-MS analysis Iridaea cordata Rhodophyta Rangel, Karen C. Debonsi, Hosana M. Clementino, Leandro C. Graminha, Marcia A. S. Vilela, Leonardo Z. Colepicolo, Pio Gaspar, Lorena R. Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
topic_facet |
Cytotoxicity Leishmanicidal Leishmania amazonensis GC-MS analysis Iridaea cordata Rhodophyta |
description |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Processo FAPESP: 2017/03552-5 Processo FAPESP: 2016/06931-4 CNPq: 407588/2013-2 Leishmaniasis is considered a neglected disease and affects billions around the world, currently presenting few therapeutic options, which makes the development of new antileishmanial drugs urgent. Secondary metabolites from marine and terrestrial organisms are important sources of new chemical entities. Herein, the potential activity of crude extracts and fractions from the Antarctic macroalga Iridaea cordata (Turner) Bory de Saint-Vincent was studied against promastigote and intracellular amastigotes forms of Leishmania amazonensis. The cytotoxicity of the active fractions was evaluated on macrophages and 3T3 BALB/c fibroblasts. The chemical profile of volatile substances was analyzed using the GC-MS technique. The fractions IC-FE (IC50-AMA = 23.6 +/- 3.4 mu g mL(-1); SI > 11) and IC-FF (IC50-AMA = 12.4 +/- 1.2 mu g mL(-1); SI > 24) showed promising activity against amastigotes and higher selectivity to the parasite rather than to the mammalian host cells, when compared to the reference drug amphotericin B (IC50-AMA = 5.9 +/- 0.3 mu g mL(-1); SI = 3.9). Their estimated LD50 in rodents are also higher than that in amphotericin B (LD50 IC-FE = 594.5 +/- 25.87 mg kg(-1); LD50 IC-FE = 580.1 +/- 11.84 mg kg(-1); LD50 amphoter = 172.20 +/- 2.40 mg kg(-1)). The chemical profile of these fractions showed the presence of phthalates, esters, ketones, fatty acids, and carboxylic acids, which might be contributing alone or synergistically to the observed antileishmanial activity. Consequently, I. cordata might be used to identify new antileishmanial compounds. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Article in Journal/Newspaper |
author |
Rangel, Karen C. Debonsi, Hosana M. Clementino, Leandro C. Graminha, Marcia A. S. Vilela, Leonardo Z. Colepicolo, Pio Gaspar, Lorena R. |
author_facet |
Rangel, Karen C. Debonsi, Hosana M. Clementino, Leandro C. Graminha, Marcia A. S. Vilela, Leonardo Z. Colepicolo, Pio Gaspar, Lorena R. |
author_sort |
Rangel, Karen C. |
title |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
title_short |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
title_full |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
title_fullStr |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
title_full_unstemmed |
Antileishmanial activity of the Antarctic red algae Iridaea cordata (Gigartinaceae; Rhodophyta) |
title_sort |
antileishmanial activity of the antarctic red algae iridaea cordata (gigartinaceae; rhodophyta) |
publisher |
Springer |
publishDate |
2019 |
url |
http://hdl.handle.net/11449/185605 https://doi.org/10.1007/s10811-018-1592-1 |
long_lat |
ENVELOPE(40.372,40.372,64.489,64.489) ENVELOPE(-59.515,-59.515,50.600,50.600) |
geographic |
Antarctic Bory Saint-Vincent The Antarctic |
geographic_facet |
Antarctic Bory Saint-Vincent The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Journal Of Applied Phycology http://dx.doi.org/10.1007/s10811-018-1592-1 Journal Of Applied Phycology. Dordrecht: Springer, v. 31, n. 2, p. 825-834, 2019. 0921-8971 http://hdl.handle.net/11449/185605 doi:10.1007/s10811-018-1592-1 WOS:000463988500002 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1007/s10811-018-1592-1 |
container_title |
Journal of Applied Phycology |
container_volume |
31 |
container_issue |
2 |
container_start_page |
825 |
op_container_end_page |
834 |
_version_ |
1770273233113710592 |