Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases
In order to improve economic viability of an enzymatic process, the use of an operationally stable and low-cost biocatalyst is encouraged. Although the immobilization of lipases is widely reported, the search for new supports and immobilization protocols with better properties is still important. In...
Published in: | Journal of Molecular Catalysis B: Enzymatic |
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Online Access: | http://hdl.handle.net/11449/174462 https://doi.org/10.1016/j.molcatb.2017.04.002 |
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ftunivespir:oai:repositorio.unesp.br:11449/174462 2023-07-02T03:29:41+02:00 Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases de Lima, Lionete Nunes Vieira, Gustavo Nakamura Alves Kopp, Willian Tardioli, Paulo Waldir Giordano, Raquel L.C. Universidade Estadual Paulista (UNESP) 2016-10-29 S491-S499 http://hdl.handle.net/11449/174462 https://doi.org/10.1016/j.molcatb.2017.04.002 eng eng Journal of Molecular Catalysis B: Enzymatic 0,522 http://dx.doi.org/10.1016/j.molcatb.2017.04.002 Journal of Molecular Catalysis B: Enzymatic, v. 133, p. S491-S499. 1873-3158 1381-1177 http://hdl.handle.net/11449/174462 doi:10.1016/j.molcatb.2017.04.002 2-s2.0-85017435486 2-s2.0-85017435486.pdf openAccess Candida antarctica lipase B Heterofunctionalization Silica magnetic microparticles Xylose fatty acid ester info:eu-repo/semantics/article 2016 ftunivespir https://doi.org/10.1016/j.molcatb.2017.04.002 2023-06-12T17:07:48Z In order to improve economic viability of an enzymatic process, the use of an operationally stable and low-cost biocatalyst is encouraged. Although the immobilization of lipases is widely reported, the search for new supports and immobilization protocols with better properties is still important. In this study, mono- and heterofunctionalized silica magnetic microparticles (SMMPs) were synthetized for immobilization of lipase B from Candida antarctica (CALB). The SMMPs were prepared in a micro-emulsion system containing sodium silicate and superparamagnetic iron oxide nanoparticles, followed by chemical modification with octyl groups and octyl plus aldehyde groups. These supports allowed the immobilization of CALB by hydrophobic adsorption or hydrophobic/covalent linkages, achieving immobilization yield of 88% and recovered activities of 128% and 59%, respectively. The performance of the magnetic biocatalysts was evaluated in the synthesis of xylose fatty acid esters (laurate or oleate) in tert-butyl alcohol medium, yielding around 60% conversion after 48 h under optimized conditions (xylose/fatty acid molar ratio of 1:0.2, 55 °C, and activity load of 37.5 U/g). The magnetic biocatalyst was used in 10 reaction cycles of 48 h at 46 °C maintaining high xylose conversions. Besides, the biocatalyst might be easily and quickly recovered from the reaction medium by an external magnetic field, an operational advantage in the case of viscous and complex media, e.g., medium containing insoluble sugars and molecular sieves. Article in Journal/Newspaper Antarc* Antarctica Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP Journal of Molecular Catalysis B: Enzymatic 133 S491 S499 |
institution |
Open Polar |
collection |
Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
op_collection_id |
ftunivespir |
language |
English |
topic |
Candida antarctica lipase B Heterofunctionalization Silica magnetic microparticles Xylose fatty acid ester |
spellingShingle |
Candida antarctica lipase B Heterofunctionalization Silica magnetic microparticles Xylose fatty acid ester de Lima, Lionete Nunes Vieira, Gustavo Nakamura Alves Kopp, Willian Tardioli, Paulo Waldir Giordano, Raquel L.C. Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
topic_facet |
Candida antarctica lipase B Heterofunctionalization Silica magnetic microparticles Xylose fatty acid ester |
description |
In order to improve economic viability of an enzymatic process, the use of an operationally stable and low-cost biocatalyst is encouraged. Although the immobilization of lipases is widely reported, the search for new supports and immobilization protocols with better properties is still important. In this study, mono- and heterofunctionalized silica magnetic microparticles (SMMPs) were synthetized for immobilization of lipase B from Candida antarctica (CALB). The SMMPs were prepared in a micro-emulsion system containing sodium silicate and superparamagnetic iron oxide nanoparticles, followed by chemical modification with octyl groups and octyl plus aldehyde groups. These supports allowed the immobilization of CALB by hydrophobic adsorption or hydrophobic/covalent linkages, achieving immobilization yield of 88% and recovered activities of 128% and 59%, respectively. The performance of the magnetic biocatalysts was evaluated in the synthesis of xylose fatty acid esters (laurate or oleate) in tert-butyl alcohol medium, yielding around 60% conversion after 48 h under optimized conditions (xylose/fatty acid molar ratio of 1:0.2, 55 °C, and activity load of 37.5 U/g). The magnetic biocatalyst was used in 10 reaction cycles of 48 h at 46 °C maintaining high xylose conversions. Besides, the biocatalyst might be easily and quickly recovered from the reaction medium by an external magnetic field, an operational advantage in the case of viscous and complex media, e.g., medium containing insoluble sugars and molecular sieves. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Article in Journal/Newspaper |
author |
de Lima, Lionete Nunes Vieira, Gustavo Nakamura Alves Kopp, Willian Tardioli, Paulo Waldir Giordano, Raquel L.C. |
author_facet |
de Lima, Lionete Nunes Vieira, Gustavo Nakamura Alves Kopp, Willian Tardioli, Paulo Waldir Giordano, Raquel L.C. |
author_sort |
de Lima, Lionete Nunes |
title |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
title_short |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
title_full |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
title_fullStr |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
title_full_unstemmed |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
title_sort |
mono- and heterofunctionalized silica magnetic microparticles (smmps) as new carriers for immobilization of lipases |
publishDate |
2016 |
url |
http://hdl.handle.net/11449/174462 https://doi.org/10.1016/j.molcatb.2017.04.002 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Journal of Molecular Catalysis B: Enzymatic 0,522 http://dx.doi.org/10.1016/j.molcatb.2017.04.002 Journal of Molecular Catalysis B: Enzymatic, v. 133, p. S491-S499. 1873-3158 1381-1177 http://hdl.handle.net/11449/174462 doi:10.1016/j.molcatb.2017.04.002 2-s2.0-85017435486 2-s2.0-85017435486.pdf |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1016/j.molcatb.2017.04.002 |
container_title |
Journal of Molecular Catalysis B: Enzymatic |
container_volume |
133 |
container_start_page |
S491 |
op_container_end_page |
S499 |
_version_ |
1770271868053356544 |