Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil
Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant resear...
Published in: | Revista Brasileira de Ciência Avícola |
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Online Access: | http://hdl.handle.net/11449/170942 https://doi.org/10.1590/1806-9061-2017-0589 |
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ftunivespir:oai:repositorio.unesp.br:11449/170942 2023-07-02T03:33:17+02:00 Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil Guerreiro, A. N. Moraes, C. C.G. Marinho, A. N.R. Barros, B. C.V. Bezerra, D. A.M. Bandeira, R. S. Silva, R. R. Rocha, D. C.C. Meneses, A. M.C. Luz, M. A. Paz, G. S. Mascarenhas, Joana D’Arc Pereira Universidade Estadual Paulista (UNESP) 2018-01-01 161-168 http://hdl.handle.net/11449/170942 https://doi.org/10.1590/1806-9061-2017-0589 eng eng Revista Brasileira de Ciencia Avicola http://dx.doi.org/10.1590/1806-9061-2017-0589 Revista Brasileira de Ciencia Avicola, v. 20, n. 1, p. 161-168, 2018. 1516-635X http://hdl.handle.net/11449/170942 doi:10.1590/1806-9061-2017-0589 S1516-635X2018000100161 2-s2.0-85046073213 S1516-635X2018000100161.pdf openAccess Amazônia Animals Picobirnavirus Reovirus Rotavirus RT-PCR info:eu-repo/semantics/article 2018 ftunivespir https://doi.org/10.1590/1806-9061-2017-0589 2023-06-12T17:05:49Z Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities. Article in Journal/Newspaper Numenius phaeopus Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP Revista Brasileira de Ciência Avícola 20 1 161 168 |
institution |
Open Polar |
collection |
Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
op_collection_id |
ftunivespir |
language |
English |
topic |
Amazônia Animals Picobirnavirus Reovirus Rotavirus RT-PCR |
spellingShingle |
Amazônia Animals Picobirnavirus Reovirus Rotavirus RT-PCR Guerreiro, A. N. Moraes, C. C.G. Marinho, A. N.R. Barros, B. C.V. Bezerra, D. A.M. Bandeira, R. S. Silva, R. R. Rocha, D. C.C. Meneses, A. M.C. Luz, M. A. Paz, G. S. Mascarenhas, Joana D’Arc Pereira Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
topic_facet |
Amazônia Animals Picobirnavirus Reovirus Rotavirus RT-PCR |
description |
Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Article in Journal/Newspaper |
author |
Guerreiro, A. N. Moraes, C. C.G. Marinho, A. N.R. Barros, B. C.V. Bezerra, D. A.M. Bandeira, R. S. Silva, R. R. Rocha, D. C.C. Meneses, A. M.C. Luz, M. A. Paz, G. S. Mascarenhas, Joana D’Arc Pereira |
author_facet |
Guerreiro, A. N. Moraes, C. C.G. Marinho, A. N.R. Barros, B. C.V. Bezerra, D. A.M. Bandeira, R. S. Silva, R. R. Rocha, D. C.C. Meneses, A. M.C. Luz, M. A. Paz, G. S. Mascarenhas, Joana D’Arc Pereira |
author_sort |
Guerreiro, A. N. |
title |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
title_short |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
title_full |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
title_fullStr |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
title_full_unstemmed |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
title_sort |
investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of pará, brazil |
publishDate |
2018 |
url |
http://hdl.handle.net/11449/170942 https://doi.org/10.1590/1806-9061-2017-0589 |
genre |
Numenius phaeopus |
genre_facet |
Numenius phaeopus |
op_relation |
Revista Brasileira de Ciencia Avicola http://dx.doi.org/10.1590/1806-9061-2017-0589 Revista Brasileira de Ciencia Avicola, v. 20, n. 1, p. 161-168, 2018. 1516-635X http://hdl.handle.net/11449/170942 doi:10.1590/1806-9061-2017-0589 S1516-635X2018000100161 2-s2.0-85046073213 S1516-635X2018000100161.pdf |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1590/1806-9061-2017-0589 |
container_title |
Revista Brasileira de Ciência Avícola |
container_volume |
20 |
container_issue |
1 |
container_start_page |
161 |
op_container_end_page |
168 |
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1770273168816078848 |