Extracellular serine proteases by Acremonium sp. L1-4B isolated from Antarctica: Overproduction using cactus pear extract with response surface methodology
Acremonium sp. L1-4B isolated from lichen in Antarctica was used to produce extracellular proteases through submerged fermentation using cactus pear extract (Opuntia ficus-indica Mill.). A 2(3) factorial design was applied to optimize the protease production using three independent variables, namely...
Published in: | Biocatalysis and Agricultural Biotechnology |
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2015
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Online Access: | http://hdl.handle.net/11449/165053 https://doi.org/10.1016/j.bcab.2015.10.006 |
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ftunivespir:oai:repositorio.unesp.br:11449/165053 2023-07-02T03:29:47+02:00 Extracellular serine proteases by Acremonium sp. L1-4B isolated from Antarctica: Overproduction using cactus pear extract with response surface methodology Evaristo da Silva Nascimento, Talita Camila Sena, Amanda Reges de Galindo Gomes, Jose Erick Santos, Wellington Leal dos Agamez Montalvo, Gualberto Segundo Tambourgi, Elias Basile Medeiros, Erika Valente de Sette, Lara Duraes Pessoa Junior, Adalberto Moreira, Keila Aparecida Universidade Estadual Paulista (UNESP) 2015-10-01 737-744 http://hdl.handle.net/11449/165053 https://doi.org/10.1016/j.bcab.2015.10.006 eng eng Elsevier B.V. Biocatalysis And Agricultural Biotechnology 0,479 http://dx.doi.org/10.1016/j.bcab.2015.10.006 Biocatalysis And Agricultural Biotechnology. Amsterdam: Elsevier Science Bv, v. 4, n. 4, p. 737-744, 2015. 1878-8181 http://hdl.handle.net/11449/165053 doi:10.1016/j.bcab.2015.10.006 WOS:000369179400042 WOS000369179400042.pdf openAccess Filamentous fungus Psychrophilic microorganisms Cactus Optimization Bioconversion info:eu-repo/semantics/article 2015 ftunivespir https://doi.org/10.1016/j.bcab.2015.10.006 2023-06-12T17:02:40Z Acremonium sp. L1-4B isolated from lichen in Antarctica was used to produce extracellular proteases through submerged fermentation using cactus pear extract (Opuntia ficus-indica Mill.). A 2(3) factorial design was applied to optimize the protease production using three independent variables, namely temperature, pH and concentration of yeast extract, was also used a Central Composite Design (CCD) under Response Surface Methodology (RSM). All variables and interactions analyzed in the factorial design were significant or marginally significant, a Central Composite Design was developed, and the Response Surface Methodology towards the highest point it was established. The experimental model was validated under 14 degrees C, pH 7.54, and 0.55% yeast extract, showing a protease activity of 447.65 +/- 2.6 U/mL by a prediction model of 445.48 U/mL. The enzyme showed a molecular weight of 59 kDa; it was inhibited in the presence of PMSF (serine protease); it presented optimal conditions at pH 8.0 and 50 degrees C; it remained stable at pH in the 3.0-9.0 range and between 10 and 40 degrees C; it showed a tolerance to 3000 mM NaCl as well as to surfactants, hydrogen peroxide and urea at 5%. This paper presents a proposal for an economically attractive production methodology using cactus pear as a primary source of carbon. In addition, the protease secreted by Acremonium sp. L1-4B presented a combination of biochemical characteristics that grants a promising variability of biotechnological applications. (C) 2015 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Antarc* Antarctica Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP Biocatalysis and Agricultural Biotechnology 4 4 737 744 |
institution |
Open Polar |
collection |
Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
op_collection_id |
ftunivespir |
language |
English |
topic |
Filamentous fungus Psychrophilic microorganisms Cactus Optimization Bioconversion |
spellingShingle |
Filamentous fungus Psychrophilic microorganisms Cactus Optimization Bioconversion Evaristo da Silva Nascimento, Talita Camila Sena, Amanda Reges de Galindo Gomes, Jose Erick Santos, Wellington Leal dos Agamez Montalvo, Gualberto Segundo Tambourgi, Elias Basile Medeiros, Erika Valente de Sette, Lara Duraes Pessoa Junior, Adalberto Moreira, Keila Aparecida Extracellular serine proteases by Acremonium sp. L1-4B isolated from Antarctica: Overproduction using cactus pear extract with response surface methodology |
topic_facet |
Filamentous fungus Psychrophilic microorganisms Cactus Optimization Bioconversion |
description |
Acremonium sp. L1-4B isolated from lichen in Antarctica was used to produce extracellular proteases through submerged fermentation using cactus pear extract (Opuntia ficus-indica Mill.). A 2(3) factorial design was applied to optimize the protease production using three independent variables, namely temperature, pH and concentration of yeast extract, was also used a Central Composite Design (CCD) under Response Surface Methodology (RSM). All variables and interactions analyzed in the factorial design were significant or marginally significant, a Central Composite Design was developed, and the Response Surface Methodology towards the highest point it was established. The experimental model was validated under 14 degrees C, pH 7.54, and 0.55% yeast extract, showing a protease activity of 447.65 +/- 2.6 U/mL by a prediction model of 445.48 U/mL. The enzyme showed a molecular weight of 59 kDa; it was inhibited in the presence of PMSF (serine protease); it presented optimal conditions at pH 8.0 and 50 degrees C; it remained stable at pH in the 3.0-9.0 range and between 10 and 40 degrees C; it showed a tolerance to 3000 mM NaCl as well as to surfactants, hydrogen peroxide and urea at 5%. This paper presents a proposal for an economically attractive production methodology using cactus pear as a primary source of carbon. In addition, the protease secreted by Acremonium sp. L1-4B presented a combination of biochemical characteristics that grants a promising variability of biotechnological applications. (C) 2015 Elsevier Ltd. All rights reserved. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Article in Journal/Newspaper |
author |
Evaristo da Silva Nascimento, Talita Camila Sena, Amanda Reges de Galindo Gomes, Jose Erick Santos, Wellington Leal dos Agamez Montalvo, Gualberto Segundo Tambourgi, Elias Basile Medeiros, Erika Valente de Sette, Lara Duraes Pessoa Junior, Adalberto Moreira, Keila Aparecida |
author_facet |
Evaristo da Silva Nascimento, Talita Camila Sena, Amanda Reges de Galindo Gomes, Jose Erick Santos, Wellington Leal dos Agamez Montalvo, Gualberto Segundo Tambourgi, Elias Basile Medeiros, Erika Valente de Sette, Lara Duraes Pessoa Junior, Adalberto Moreira, Keila Aparecida |
author_sort |
Evaristo da Silva Nascimento, Talita Camila |
title |
Extracellular serine proteases by Acremonium sp. L1-4B isolated from Antarctica: Overproduction using cactus pear extract with response surface methodology |
title_short |
Extracellular serine proteases by Acremonium sp. L1-4B isolated from Antarctica: Overproduction using cactus pear extract with response surface methodology |
title_full |
Extracellular serine proteases by Acremonium sp. L1-4B isolated from Antarctica: Overproduction using cactus pear extract with response surface methodology |
title_fullStr |
Extracellular serine proteases by Acremonium sp. L1-4B isolated from Antarctica: Overproduction using cactus pear extract with response surface methodology |
title_full_unstemmed |
Extracellular serine proteases by Acremonium sp. L1-4B isolated from Antarctica: Overproduction using cactus pear extract with response surface methodology |
title_sort |
extracellular serine proteases by acremonium sp. l1-4b isolated from antarctica: overproduction using cactus pear extract with response surface methodology |
publisher |
Elsevier B.V. |
publishDate |
2015 |
url |
http://hdl.handle.net/11449/165053 https://doi.org/10.1016/j.bcab.2015.10.006 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Biocatalysis And Agricultural Biotechnology 0,479 http://dx.doi.org/10.1016/j.bcab.2015.10.006 Biocatalysis And Agricultural Biotechnology. Amsterdam: Elsevier Science Bv, v. 4, n. 4, p. 737-744, 2015. 1878-8181 http://hdl.handle.net/11449/165053 doi:10.1016/j.bcab.2015.10.006 WOS:000369179400042 WOS000369179400042.pdf |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1016/j.bcab.2015.10.006 |
container_title |
Biocatalysis and Agricultural Biotechnology |
container_volume |
4 |
container_issue |
4 |
container_start_page |
737 |
op_container_end_page |
744 |
_version_ |
1770272605499031552 |