Alterations of C-MYC, NKX3.1, and E-cadherin expression in canine prostate carcinogenesis
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Processo FAPESP: 2010/13774-6 Processo FAPESP: 2012/16068-0 The dog (canis lupus familiaris) is the only other species besides humans thatdevelop spontaneous prostatic carcinomas (PCa) at a high frequency. The canine model is pri-marily u...
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Online Access: | http://hdl.handle.net/11449/140550 https://doi.org/10.1002/jemt.22292 |
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ftunivespir:oai:repositorio.unesp.br:11449/140550 2023-07-02T03:31:55+02:00 Alterations of C-MYC, NKX3.1, and E-cadherin expression in canine prostate carcinogenesis Fonseca-Alves, Carlos Eduardo Rodrigues, Marcela Marcondes Pinto Moura, Veridiana Maria Brianezi Dignani de Rogatto, Silvia Regina Laufer-Amorim, Renee Universidade Estadual Paulista (UNESP) 2013 1250-1256 http://hdl.handle.net/11449/140550 https://doi.org/10.1002/jemt.22292 eng eng Microscopy Research and Technique 1.087 http://dx.doi.org/10.1002/jemt.22292 Microscopy Research and Technique, v. 76, n. 12, p. 1250-1256, 2013. 1059-910X http://hdl.handle.net/11449/140550 doi:10.1002/jemt.22292 2259986546265579 closedAccess Canine Prostatic carcinoma Immunohistochemistry Protein expression info:eu-repo/semantics/article 2013 ftunivespir https://doi.org/10.1002/jemt.22292 2023-06-12T16:49:04Z Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Processo FAPESP: 2010/13774-6 Processo FAPESP: 2012/16068-0 The dog (canis lupus familiaris) is the only other species besides humans thatdevelop spontaneous prostatic carcinomas (PCa) at a high frequency. The canine model is pri-marily utili zed for the study of the PCa molecular mechanisms and provides a natural animalmodel for the study of potential therapies. In humans, the PCa frequently exhibits mutations inthe C-MYC and a reduced expression of the E-cadherin and NKX3.1 proteins. This study’s objec-tivewastoevaluatetheNKX3.1,C-MYC,andE-cadherinexpressioninthecaninenormalpros-tate, benign p rostatic hyperplasia (BPH), proliferative inflammatory atrophy (PIA) and PCa andto verify differences in expression and subcellula r localiz ation of these proteins in the prostaticcarcinogenesis. A tissue microarray (TMA) slide was constructed, and immunohistochemistrywith antibodies raised against C-MYC, NKX3.1, E-cadherin and p63 was performed using theperoxidase and DAB methods. The C -MYC protein expression was elevated in the cytoplasm andnuclei of t he canine PCa a nd PIA compared with the normal prostate (P 5 0.004. The NKX3.1protein expression was reduced in 94.75% o f the PCa and 100% of the PIA compared with thenormal prostate (P 5 0.0022). In fact, t he expression of E-c adherin trended towards a decrease incarcinomas when compared t o normal p rostate and PIA. By immunohistochemistry, more p63-positive basal cells were observed in the P Ca and PIA when compared with the normal prostate(P 5 0.0002). T his study has demonst rated that the carcinogenesis of canine prostatic tissue maybe related to basal cel l proliferation, the gain of C-MYC fu nc tion and the loss of NKX3.1 pr oteinexpression. Article in Journal/Newspaper Canis lupus Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP Microscopy Research and Technique 76 12 1250 1256 |
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Open Polar |
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Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
op_collection_id |
ftunivespir |
language |
English |
topic |
Canine Prostatic carcinoma Immunohistochemistry Protein expression |
spellingShingle |
Canine Prostatic carcinoma Immunohistochemistry Protein expression Fonseca-Alves, Carlos Eduardo Rodrigues, Marcela Marcondes Pinto Moura, Veridiana Maria Brianezi Dignani de Rogatto, Silvia Regina Laufer-Amorim, Renee Alterations of C-MYC, NKX3.1, and E-cadherin expression in canine prostate carcinogenesis |
topic_facet |
Canine Prostatic carcinoma Immunohistochemistry Protein expression |
description |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Processo FAPESP: 2010/13774-6 Processo FAPESP: 2012/16068-0 The dog (canis lupus familiaris) is the only other species besides humans thatdevelop spontaneous prostatic carcinomas (PCa) at a high frequency. The canine model is pri-marily utili zed for the study of the PCa molecular mechanisms and provides a natural animalmodel for the study of potential therapies. In humans, the PCa frequently exhibits mutations inthe C-MYC and a reduced expression of the E-cadherin and NKX3.1 proteins. This study’s objec-tivewastoevaluatetheNKX3.1,C-MYC,andE-cadherinexpressioninthecaninenormalpros-tate, benign p rostatic hyperplasia (BPH), proliferative inflammatory atrophy (PIA) and PCa andto verify differences in expression and subcellula r localiz ation of these proteins in the prostaticcarcinogenesis. A tissue microarray (TMA) slide was constructed, and immunohistochemistrywith antibodies raised against C-MYC, NKX3.1, E-cadherin and p63 was performed using theperoxidase and DAB methods. The C -MYC protein expression was elevated in the cytoplasm andnuclei of t he canine PCa a nd PIA compared with the normal prostate (P 5 0.004. The NKX3.1protein expression was reduced in 94.75% o f the PCa and 100% of the PIA compared with thenormal prostate (P 5 0.0022). In fact, t he expression of E-c adherin trended towards a decrease incarcinomas when compared t o normal p rostate and PIA. By immunohistochemistry, more p63-positive basal cells were observed in the P Ca and PIA when compared with the normal prostate(P 5 0.0002). T his study has demonst rated that the carcinogenesis of canine prostatic tissue maybe related to basal cel l proliferation, the gain of C-MYC fu nc tion and the loss of NKX3.1 pr oteinexpression. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Article in Journal/Newspaper |
author |
Fonseca-Alves, Carlos Eduardo Rodrigues, Marcela Marcondes Pinto Moura, Veridiana Maria Brianezi Dignani de Rogatto, Silvia Regina Laufer-Amorim, Renee |
author_facet |
Fonseca-Alves, Carlos Eduardo Rodrigues, Marcela Marcondes Pinto Moura, Veridiana Maria Brianezi Dignani de Rogatto, Silvia Regina Laufer-Amorim, Renee |
author_sort |
Fonseca-Alves, Carlos Eduardo |
title |
Alterations of C-MYC, NKX3.1, and E-cadherin expression in canine prostate carcinogenesis |
title_short |
Alterations of C-MYC, NKX3.1, and E-cadherin expression in canine prostate carcinogenesis |
title_full |
Alterations of C-MYC, NKX3.1, and E-cadherin expression in canine prostate carcinogenesis |
title_fullStr |
Alterations of C-MYC, NKX3.1, and E-cadherin expression in canine prostate carcinogenesis |
title_full_unstemmed |
Alterations of C-MYC, NKX3.1, and E-cadherin expression in canine prostate carcinogenesis |
title_sort |
alterations of c-myc, nkx3.1, and e-cadherin expression in canine prostate carcinogenesis |
publishDate |
2013 |
url |
http://hdl.handle.net/11449/140550 https://doi.org/10.1002/jemt.22292 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
Microscopy Research and Technique 1.087 http://dx.doi.org/10.1002/jemt.22292 Microscopy Research and Technique, v. 76, n. 12, p. 1250-1256, 2013. 1059-910X http://hdl.handle.net/11449/140550 doi:10.1002/jemt.22292 2259986546265579 |
op_rights |
closedAccess |
op_doi |
https://doi.org/10.1002/jemt.22292 |
container_title |
Microscopy Research and Technique |
container_volume |
76 |
container_issue |
12 |
container_start_page |
1250 |
op_container_end_page |
1256 |
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1770271371083907072 |