Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The feeding behavior of sa...
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2015
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Online Access: | http://hdl.handle.net/11449/129375 http://www.sciencedirect.com/science/article/pii/S0001706X14003593 https://doi.org/10.1016/j.actatropica.2014.11.006 |
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ftunivespir:oai:repositorio.unesp.br:11449/129375 2023-07-02T03:31:55+02:00 Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil Baum, Mauricio Castro, Edilene Alcantara de Pinto, Mara Cristina Goulart, Thais Marchi Baura, Walter Klisiowicz, Debora do Rocio Costa-Ribeiro, Magda Clara Vieira da Universidade Estadual Paulista (UNESP) 2015-03-01 8-12 http://hdl.handle.net/11449/129375 http://www.sciencedirect.com/science/article/pii/S0001706X14003593 https://doi.org/10.1016/j.actatropica.2014.11.006 eng eng Elsevier B.V. Acta Tropica 2.509 http://www.sciencedirect.com/science/article/pii/S0001706X14003593 Acta Tropica. Amsterdam: Elsevier Science Bv, v. 143, p. 8-12, 2015. 0001-706X http://hdl.handle.net/11449/129375 doi:10.1016/j.actatropica.2014.11.006 WOS:000350526800002 8221737491598415 closedAccess American cutaneous leishmaniasis Sand flies Prepronociceptin (PNOC) Host Reservoir info:eu-repo/semantics/article 2015 ftunivespir https://doi.org/10.1016/j.actatropica.2014.11.006 2023-06-12T16:42:27Z Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The feeding behavior of sand flies provides valuable information about the vector/host interactions and elucidates the epidemiological patterns of American cutaneous leishmaniasis (ACL) transmission. The aim of this study was to identify the blood meal sources of sand flies in endemic areas of leishmaniasis in Parana State through polymerase chain reaction (PCR) amplification of a prepronociceptin (PNOC) gene fragment and its subsequent DNA sequencing. Moreover, molecular assays were conducted to evaluate the sensitivity and reproducibility of the PNOC gene amplification. Besides that, a time-course digestion test of the blood using sand flies that fed artificially on BALB/c mice was performed. Of 1263 female sand flies collected in the field, 93 (3.6%) specimens were engorged and 27 allowed efficient amplification of the PNOC gene. These flies had fed on equine (Equus caballus), porcine (Sus scrofa) and canine (Canis lupus familiaris) species. The results also showed that the identification of the blood meal sources of the sand flies using the molecular method was directly linked to the level of digestion of the blood (time-course) and not to the amount of blood that had been ingested or to the presence of inhibitors in the blood. Article in Journal/Newspaper Canis lupus Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP Acta Tropica 143 8 12 |
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Open Polar |
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Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
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ftunivespir |
language |
English |
topic |
American cutaneous leishmaniasis Sand flies Prepronociceptin (PNOC) Host Reservoir |
spellingShingle |
American cutaneous leishmaniasis Sand flies Prepronociceptin (PNOC) Host Reservoir Baum, Mauricio Castro, Edilene Alcantara de Pinto, Mara Cristina Goulart, Thais Marchi Baura, Walter Klisiowicz, Debora do Rocio Costa-Ribeiro, Magda Clara Vieira da Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
topic_facet |
American cutaneous leishmaniasis Sand flies Prepronociceptin (PNOC) Host Reservoir |
description |
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The feeding behavior of sand flies provides valuable information about the vector/host interactions and elucidates the epidemiological patterns of American cutaneous leishmaniasis (ACL) transmission. The aim of this study was to identify the blood meal sources of sand flies in endemic areas of leishmaniasis in Parana State through polymerase chain reaction (PCR) amplification of a prepronociceptin (PNOC) gene fragment and its subsequent DNA sequencing. Moreover, molecular assays were conducted to evaluate the sensitivity and reproducibility of the PNOC gene amplification. Besides that, a time-course digestion test of the blood using sand flies that fed artificially on BALB/c mice was performed. Of 1263 female sand flies collected in the field, 93 (3.6%) specimens were engorged and 27 allowed efficient amplification of the PNOC gene. These flies had fed on equine (Equus caballus), porcine (Sus scrofa) and canine (Canis lupus familiaris) species. The results also showed that the identification of the blood meal sources of the sand flies using the molecular method was directly linked to the level of digestion of the blood (time-course) and not to the amount of blood that had been ingested or to the presence of inhibitors in the blood. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Article in Journal/Newspaper |
author |
Baum, Mauricio Castro, Edilene Alcantara de Pinto, Mara Cristina Goulart, Thais Marchi Baura, Walter Klisiowicz, Debora do Rocio Costa-Ribeiro, Magda Clara Vieira da |
author_facet |
Baum, Mauricio Castro, Edilene Alcantara de Pinto, Mara Cristina Goulart, Thais Marchi Baura, Walter Klisiowicz, Debora do Rocio Costa-Ribeiro, Magda Clara Vieira da |
author_sort |
Baum, Mauricio |
title |
Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
title_short |
Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
title_full |
Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
title_fullStr |
Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
title_full_unstemmed |
Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
title_sort |
molecular detection of the blood meal source of sand flies (diptera: psychodidae) in a transmission area of american cutaneous leishmaniasis, parana state, brazil |
publisher |
Elsevier B.V. |
publishDate |
2015 |
url |
http://hdl.handle.net/11449/129375 http://www.sciencedirect.com/science/article/pii/S0001706X14003593 https://doi.org/10.1016/j.actatropica.2014.11.006 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
Acta Tropica 2.509 http://www.sciencedirect.com/science/article/pii/S0001706X14003593 Acta Tropica. Amsterdam: Elsevier Science Bv, v. 143, p. 8-12, 2015. 0001-706X http://hdl.handle.net/11449/129375 doi:10.1016/j.actatropica.2014.11.006 WOS:000350526800002 8221737491598415 |
op_rights |
closedAccess |
op_doi |
https://doi.org/10.1016/j.actatropica.2014.11.006 |
container_title |
Acta Tropica |
container_volume |
143 |
container_start_page |
8 |
op_container_end_page |
12 |
_version_ |
1770271361646723072 |