Non-exhaustive test for aerobic capacity determination in running rats
A simple and applicable method for non-exhaustive aerobic evaluation in running rats is described. Wistar rats were submitted to running test at different velocities (10, 15, 20, 25 m/min) with 48 h recovery among them. At each velocity, the rats ran two bouts of 5 min with 2 min of rest between bou...
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ftunivesp:oai:acervodigital.unesp.br:11449/72724 2023-05-15T18:05:29+02:00 Non-exhaustive test for aerobic capacity determination in running rats Manchado-Gobatto, F. B. Gobatto, C. A. Contarteze, R. V L Mello, M. A R Universidade Estadual Paulista (UNESP) 2014-05-27T11:26:01Z http://acervodigital.unesp.br/handle/11449/72724 http://hdl.handle.net/11449/72724 http://www.ncbi.nlm.nih.gov/pubmed/22013745 eng eng Indian Journal of Experimental Biology Indian Journal of Experimental Biology, v. 49, n. 10, p. 781-785, 2011. 0019-5189 http://hdl.handle.net/11449/72724 http://acervodigital.unesp.br/handle/11449/72724 2-s2.0-80054019319 http://www.ncbi.nlm.nih.gov/pubmed/22013745 info:eu-repo/semantics/closedAccess Blood lactate Critical load Double bouts exercise test Maximal lactate steady state Running lactic acid animal animal experiment blood exercise test male oxygen consumption physiology rat Wistar rat Animals Physical Conditioning Rats Wistar Animalia Rattus Rattus norvegicus outro 2014 ftunivesp 2021-07-18T08:41:59Z A simple and applicable method for non-exhaustive aerobic evaluation in running rats is described. Wistar rats were submitted to running test at different velocities (10, 15, 20, 25 m/min) with 48 h recovery among them. At each velocity, the rats ran two bouts of 5 min with 2 min of rest between bouts. Blood samples were collected at the end of each bout for lactate determination. For each intensity, delta lactate was calculated and using deltas obtained by four tests, an individual linear interpolation was plotted. The y-intercept of linear interpolation was the null delta lactate equivalent to the critical velocity (CV). To verify the lactate stabilization at CV, the animals were submitted to 25 min of continuous exercise (15, 20, 25 m/min), with blood collection every 5 min. The estimated CV was 16.6±0.7 m/min, with significant linear regressions (R=0.90±0.03). The rats presented maximal lactate steady state (MLSS) at 3.9±0.4 mmol/L, at 20 m/min. The CV was less than MLSS but significantly correlated with this parameter (r=0.78). This non-exhaustive test seems to be valid for the aerobic evaluation of sedentary rats and this protocol underestimates the MLSS in 20%. This test seems to be the interesting method for the evaluation of rats submitted to acute exercise or physical training. Other/Unknown Material Rattus rattus Universidade Estadual Paulista São Paulo: Acervo Digital da UNESP / São Paulo State University The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) |
institution |
Open Polar |
collection |
Universidade Estadual Paulista São Paulo: Acervo Digital da UNESP / São Paulo State University |
op_collection_id |
ftunivesp |
language |
English |
topic |
Blood lactate Critical load Double bouts exercise test Maximal lactate steady state Running lactic acid animal animal experiment blood exercise test male oxygen consumption physiology rat Wistar rat Animals Physical Conditioning Rats Wistar Animalia Rattus Rattus norvegicus |
spellingShingle |
Blood lactate Critical load Double bouts exercise test Maximal lactate steady state Running lactic acid animal animal experiment blood exercise test male oxygen consumption physiology rat Wistar rat Animals Physical Conditioning Rats Wistar Animalia Rattus Rattus norvegicus Manchado-Gobatto, F. B. Gobatto, C. A. Contarteze, R. V L Mello, M. A R Non-exhaustive test for aerobic capacity determination in running rats |
topic_facet |
Blood lactate Critical load Double bouts exercise test Maximal lactate steady state Running lactic acid animal animal experiment blood exercise test male oxygen consumption physiology rat Wistar rat Animals Physical Conditioning Rats Wistar Animalia Rattus Rattus norvegicus |
description |
A simple and applicable method for non-exhaustive aerobic evaluation in running rats is described. Wistar rats were submitted to running test at different velocities (10, 15, 20, 25 m/min) with 48 h recovery among them. At each velocity, the rats ran two bouts of 5 min with 2 min of rest between bouts. Blood samples were collected at the end of each bout for lactate determination. For each intensity, delta lactate was calculated and using deltas obtained by four tests, an individual linear interpolation was plotted. The y-intercept of linear interpolation was the null delta lactate equivalent to the critical velocity (CV). To verify the lactate stabilization at CV, the animals were submitted to 25 min of continuous exercise (15, 20, 25 m/min), with blood collection every 5 min. The estimated CV was 16.6±0.7 m/min, with significant linear regressions (R=0.90±0.03). The rats presented maximal lactate steady state (MLSS) at 3.9±0.4 mmol/L, at 20 m/min. The CV was less than MLSS but significantly correlated with this parameter (r=0.78). This non-exhaustive test seems to be valid for the aerobic evaluation of sedentary rats and this protocol underestimates the MLSS in 20%. This test seems to be the interesting method for the evaluation of rats submitted to acute exercise or physical training. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Other/Unknown Material |
author |
Manchado-Gobatto, F. B. Gobatto, C. A. Contarteze, R. V L Mello, M. A R |
author_facet |
Manchado-Gobatto, F. B. Gobatto, C. A. Contarteze, R. V L Mello, M. A R |
author_sort |
Manchado-Gobatto, F. B. |
title |
Non-exhaustive test for aerobic capacity determination in running rats |
title_short |
Non-exhaustive test for aerobic capacity determination in running rats |
title_full |
Non-exhaustive test for aerobic capacity determination in running rats |
title_fullStr |
Non-exhaustive test for aerobic capacity determination in running rats |
title_full_unstemmed |
Non-exhaustive test for aerobic capacity determination in running rats |
title_sort |
non-exhaustive test for aerobic capacity determination in running rats |
publishDate |
2014 |
url |
http://acervodigital.unesp.br/handle/11449/72724 http://hdl.handle.net/11449/72724 http://www.ncbi.nlm.nih.gov/pubmed/22013745 |
long_lat |
ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
The ''Y'' |
geographic_facet |
The ''Y'' |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
Indian Journal of Experimental Biology Indian Journal of Experimental Biology, v. 49, n. 10, p. 781-785, 2011. 0019-5189 http://hdl.handle.net/11449/72724 http://acervodigital.unesp.br/handle/11449/72724 2-s2.0-80054019319 http://www.ncbi.nlm.nih.gov/pubmed/22013745 |
op_rights |
info:eu-repo/semantics/closedAccess |
_version_ |
1766176967365754880 |