Could handling and acclimatization affect biomarker responses in oysters?
The aim of this study was to investigate whether handling and acclimatization could affect the biomarker responses in oysters Crassostrea gigas. Adult oysters were sampled in a farming area, subjected to handling stress during two hours (shell cleaning and transport), and then acclimatized in labora...
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Online Access: | http://acervodigital.unesp.br/handle/11449/70447 http://hdl.handle.net/11449/70447 https://doi.org/10.1016/j.marenvres.2008.02.052 |
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ftunivesp:oai:acervodigital.unesp.br:11449/70447 2023-05-15T15:58:56+02:00 Could handling and acclimatization affect biomarker responses in oysters? Zanette, Juliano Freitas, Fernanda Almeida de Silva, Angela Zaccaron da Moser, Righetto Almeida, Eduardo Alves de Marques, Maria Risoleta Freire Bainy, Celso Dias Universidade Estadual Paulista (UNESP) 2014-05-27T11:23:35Z http://acervodigital.unesp.br/handle/11449/70447 http://hdl.handle.net/11449/70447 https://doi.org/10.1016/j.marenvres.2008.02.052 eng eng Marine Environmental Research Marine Environmental Research, v. 66, n. 1, p. 172-, 2008. 0141-1136 http://hdl.handle.net/11449/70447 http://acervodigital.unesp.br/handle/11449/70447 doi:10.1016/j.marenvres.2008.02.052 2-s2.0-45349098847 http://dx.doi.org/10.1016/j.marenvres.2008.02.052 info:eu-repo/semantics/closedAccess outro 2014 ftunivesp https://doi.org/10.1016/j.marenvres.2008.02.052 2021-07-18T08:40:52Z The aim of this study was to investigate whether handling and acclimatization could affect the biomarker responses in oysters Crassostrea gigas. Adult oysters were sampled in a farming area, subjected to handling stress during two hours (shell cleaning and transport), and then acclimatized in laboratory for 2, 3 and 4 weeks. Groups of five oysters were sampled before and after the handling (T0 and T1, respectively), and after 2, 3 and 4 weeks acclimatization. During the acclimatization, water was renewed daily, food given twice a day and temperature and salinity maintained at 22 °C and 25 ppt, respectively. One group, in another tank, was kept in similar conditions and was exposed for 1 week to 0.1 % diesel after the 2-weeks acclimatization period. After exposure, gills were immediately frozen in liquid N 2 for biochemical analyses. Higher expression of heat-shock proteins (HSP70) was observed after handling, and after acclimatization periods of 3-week and 4-week, compared to the T0 group. The diesel exposed group did not show elevated levels of HSP70, when compared to the 3-week acclimatized group. The activity of glutathione S-transferase (GST) was unchanged after handling, but was lower after all acclimatization periods, compared to the T0 group. Exposure to diesel caused an increase in GST activity compared to the 3-week acclimatized group, but not compared to T0. The activity of catalase (CAT), acetylcholinesterase (AChE), and the MDA levels remained unchanged during the whole experiment. These results point to the need of a special care in laboratory and field experiments employing HSP70 and GST as biomarkers. (Supported by CNPq-CTPetro to ACDB.). © 2008 Published by Elsevier Ltd. Other/Unknown Material Crassostrea gigas Universidade Estadual Paulista São Paulo: Acervo Digital da UNESP / São Paulo State University Marine Environmental Research 66 1 187 189 |
institution |
Open Polar |
collection |
Universidade Estadual Paulista São Paulo: Acervo Digital da UNESP / São Paulo State University |
op_collection_id |
ftunivesp |
language |
English |
description |
The aim of this study was to investigate whether handling and acclimatization could affect the biomarker responses in oysters Crassostrea gigas. Adult oysters were sampled in a farming area, subjected to handling stress during two hours (shell cleaning and transport), and then acclimatized in laboratory for 2, 3 and 4 weeks. Groups of five oysters were sampled before and after the handling (T0 and T1, respectively), and after 2, 3 and 4 weeks acclimatization. During the acclimatization, water was renewed daily, food given twice a day and temperature and salinity maintained at 22 °C and 25 ppt, respectively. One group, in another tank, was kept in similar conditions and was exposed for 1 week to 0.1 % diesel after the 2-weeks acclimatization period. After exposure, gills were immediately frozen in liquid N 2 for biochemical analyses. Higher expression of heat-shock proteins (HSP70) was observed after handling, and after acclimatization periods of 3-week and 4-week, compared to the T0 group. The diesel exposed group did not show elevated levels of HSP70, when compared to the 3-week acclimatized group. The activity of glutathione S-transferase (GST) was unchanged after handling, but was lower after all acclimatization periods, compared to the T0 group. Exposure to diesel caused an increase in GST activity compared to the 3-week acclimatized group, but not compared to T0. The activity of catalase (CAT), acetylcholinesterase (AChE), and the MDA levels remained unchanged during the whole experiment. These results point to the need of a special care in laboratory and field experiments employing HSP70 and GST as biomarkers. (Supported by CNPq-CTPetro to ACDB.). © 2008 Published by Elsevier Ltd. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Other/Unknown Material |
author |
Zanette, Juliano Freitas, Fernanda Almeida de Silva, Angela Zaccaron da Moser, Righetto Almeida, Eduardo Alves de Marques, Maria Risoleta Freire Bainy, Celso Dias |
spellingShingle |
Zanette, Juliano Freitas, Fernanda Almeida de Silva, Angela Zaccaron da Moser, Righetto Almeida, Eduardo Alves de Marques, Maria Risoleta Freire Bainy, Celso Dias Could handling and acclimatization affect biomarker responses in oysters? |
author_facet |
Zanette, Juliano Freitas, Fernanda Almeida de Silva, Angela Zaccaron da Moser, Righetto Almeida, Eduardo Alves de Marques, Maria Risoleta Freire Bainy, Celso Dias |
author_sort |
Zanette, Juliano |
title |
Could handling and acclimatization affect biomarker responses in oysters? |
title_short |
Could handling and acclimatization affect biomarker responses in oysters? |
title_full |
Could handling and acclimatization affect biomarker responses in oysters? |
title_fullStr |
Could handling and acclimatization affect biomarker responses in oysters? |
title_full_unstemmed |
Could handling and acclimatization affect biomarker responses in oysters? |
title_sort |
could handling and acclimatization affect biomarker responses in oysters? |
publishDate |
2014 |
url |
http://acervodigital.unesp.br/handle/11449/70447 http://hdl.handle.net/11449/70447 https://doi.org/10.1016/j.marenvres.2008.02.052 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
Marine Environmental Research Marine Environmental Research, v. 66, n. 1, p. 172-, 2008. 0141-1136 http://hdl.handle.net/11449/70447 http://acervodigital.unesp.br/handle/11449/70447 doi:10.1016/j.marenvres.2008.02.052 2-s2.0-45349098847 http://dx.doi.org/10.1016/j.marenvres.2008.02.052 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.marenvres.2008.02.052 |
container_title |
Marine Environmental Research |
container_volume |
66 |
container_issue |
1 |
container_start_page |
187 |
op_container_end_page |
189 |
_version_ |
1766394713769771008 |