Assessment of the potential clastogenic/aneugenic risk of Casearia sylvestris extract using in vivo assays

Casearia sylvestris (Flacourtiaceae) is a plant which grows in wild and has been widely used in folk medicine. In this study, clastogenic/aneugenic properties of Casearia sylvestris crude ethanolic extract were evaluated using in vivo chromosomal aberrations (CAs) and micronucleus (MN) assays in rod...

Full description

Bibliographic Details
Published in:CYTOLOGIA
Main Authors: Maistro, Edson Luis, De Andrade, Sergio Faloni, Puras, Marilane Aparecida
Other Authors: Universidade Estadual Paulista (UNESP)
Format: Other/Unknown Material
Language:English
Published: 2014
Subjects:
Casearia sylvestris
Chromosome aberration
In vivo
Micronucleus test
Animalia
Casearia
Flacourtiaceae
Mus
Rattus
Rattus norvegicus
Rodentia
Rattus rattus
Online Access:http://acervodigital.unesp.br/handle/11449/70044
http://hdl.handle.net/11449/70044
https://doi.org/10.1508/cytologia.72.401
Description
Summary:Casearia sylvestris (Flacourtiaceae) is a plant which grows in wild and has been widely used in folk medicine. In this study, clastogenic/aneugenic properties of Casearia sylvestris crude ethanolic extract were evaluated using in vivo chromosomal aberrations (CAs) and micronucleus (MN) assays in rodents. The animals were treated by gavage with 3 concentrations of the extract: 150, 300 and 500 mg/kg body weight. Bone marrow cells from Wistar rats were collected 24 h after having been submitted to the MN and CAs test. Peripheral blood cells from Swiss mice were collected 48 and 72 h after having been submitted to the MN test. The results show that C. sylvestris extract does not induce a significant increase in mean values for micronucleated polychromatic erythrocytes (MNPCE) in Swiss mice and Wistar rats, or CAs in rat bone marrow cells, at the 3 tested doses, indicating that the extract showed no clastogenic/aneugenic effects on chromosomes of the rodent cells tested. © 2007 The Japan Mendel Society.

Similar Items