Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7
The production, purification, and characterization of an extracellular protease released by Rhodotorula mucilaginosa L7 were evaluated in this study. This strain was isolated from an Antarctic marine alga and previously selected among others based on the capacity to produce the highest extracellular...
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Elsevier B. V.
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ftunivesp:oai:acervodigital.unesp.br:11449/131262 2023-05-15T13:59:03+02:00 Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 Lario, Luciana Daniela Chaud, Luciana Almeida, María das Graças Converti, Attilio Durães Sette, Lara Pessoa, Adalberto Universidade Estadual Paulista (UNESP) 2015-12-07T15:33:10Z http://acervodigital.unesp.br/handle/11449/131262 http://hdl.handle.net/11449/131262 https://doi.org/10.1016/j.funbio.2015.08.012 eng eng Elsevier B. V. Fungal Biology Fungal Biology, v. 119, n. 11, p. 1129-1136, 2015. 1878-6146 http://hdl.handle.net/11449/131262 http://acervodigital.unesp.br/handle/11449/131262 doi:10.1016/j.funbio.2015.08.012 26466885 http://dx.doi.org/10.1016/j.funbio.2015.08.012 info:eu-repo/semantics/closedAccess Cold-adapted yeast Enzyme characterization Extracellular protease outro 2015 ftunivesp https://doi.org/10.1016/j.funbio.2015.08.012 2021-07-18T09:08:20Z The production, purification, and characterization of an extracellular protease released by Rhodotorula mucilaginosa L7 were evaluated in this study. This strain was isolated from an Antarctic marine alga and previously selected among others based on the capacity to produce the highest extracellular proteolytic activity in preliminary tests. R. mucilaginosa L7 was grown in Saboraud-dextrose medium at 25 °C, and the cell growth, pH of the medium, extracellular protease production and the glucose and protein consumption were determined as a function of time. The protease was then purified, and the effects of pH, temperature, and salt concentration on the catalytic activity and enzyme stability were determined. Enzyme production started at the beginning of the exponential phase of growth and reached a maximum after 48 h, which was accompanied by a decrease in the pH as well as reductions of the protein and glucose concentrations in the medium. The purified protease presented optimal catalytic activity at pH 5.0 and 50 °C. Finally, the enzyme was stable in the presence of high concentrations of NaCl. These characteristics are of interest for future studies and may lead to potential biotechnological applications that require enzyme activity and stability under acidic conditions and/or high salt concentrations. Other/Unknown Material Antarc* Antarctic Universidade Estadual Paulista São Paulo: Acervo Digital da UNESP / São Paulo State University Antarctic Fungal Biology 119 11 1129 1136 |
institution |
Open Polar |
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Universidade Estadual Paulista São Paulo: Acervo Digital da UNESP / São Paulo State University |
op_collection_id |
ftunivesp |
language |
English |
topic |
Cold-adapted yeast Enzyme characterization Extracellular protease |
spellingShingle |
Cold-adapted yeast Enzyme characterization Extracellular protease Lario, Luciana Daniela Chaud, Luciana Almeida, María das Graças Converti, Attilio Durães Sette, Lara Pessoa, Adalberto Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
topic_facet |
Cold-adapted yeast Enzyme characterization Extracellular protease |
description |
The production, purification, and characterization of an extracellular protease released by Rhodotorula mucilaginosa L7 were evaluated in this study. This strain was isolated from an Antarctic marine alga and previously selected among others based on the capacity to produce the highest extracellular proteolytic activity in preliminary tests. R. mucilaginosa L7 was grown in Saboraud-dextrose medium at 25 °C, and the cell growth, pH of the medium, extracellular protease production and the glucose and protein consumption were determined as a function of time. The protease was then purified, and the effects of pH, temperature, and salt concentration on the catalytic activity and enzyme stability were determined. Enzyme production started at the beginning of the exponential phase of growth and reached a maximum after 48 h, which was accompanied by a decrease in the pH as well as reductions of the protein and glucose concentrations in the medium. The purified protease presented optimal catalytic activity at pH 5.0 and 50 °C. Finally, the enzyme was stable in the presence of high concentrations of NaCl. These characteristics are of interest for future studies and may lead to potential biotechnological applications that require enzyme activity and stability under acidic conditions and/or high salt concentrations. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Other/Unknown Material |
author |
Lario, Luciana Daniela Chaud, Luciana Almeida, María das Graças Converti, Attilio Durães Sette, Lara Pessoa, Adalberto |
author_facet |
Lario, Luciana Daniela Chaud, Luciana Almeida, María das Graças Converti, Attilio Durães Sette, Lara Pessoa, Adalberto |
author_sort |
Lario, Luciana Daniela |
title |
Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
title_short |
Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
title_full |
Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
title_fullStr |
Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
title_full_unstemmed |
Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
title_sort |
production, purification, and characterization of an extracellular acid protease from the marine antarctic yeast rhodotorula mucilaginosa l7 |
publisher |
Elsevier B. V. |
publishDate |
2015 |
url |
http://acervodigital.unesp.br/handle/11449/131262 http://hdl.handle.net/11449/131262 https://doi.org/10.1016/j.funbio.2015.08.012 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Fungal Biology Fungal Biology, v. 119, n. 11, p. 1129-1136, 2015. 1878-6146 http://hdl.handle.net/11449/131262 http://acervodigital.unesp.br/handle/11449/131262 doi:10.1016/j.funbio.2015.08.012 26466885 http://dx.doi.org/10.1016/j.funbio.2015.08.012 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.funbio.2015.08.012 |
container_title |
Fungal Biology |
container_volume |
119 |
container_issue |
11 |
container_start_page |
1129 |
op_container_end_page |
1136 |
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1766267415209967616 |