Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil
The feeding behavior of sand flies provides valuable information about the vector/host interactions and elucidates the epidemiological patterns of American cutaneous leishmaniasis (ACL) transmission. The aim of this study was to identify the blood meal sources of sand flies in endemic areas of leish...
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Elsevier B.V.
2015
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Online Access: | http://acervodigital.unesp.br/handle/11449/129375 http://hdl.handle.net/11449/129375 https://doi.org/10.1016/j.actatropica.2014.11.006 http://www.sciencedirect.com/science/article/pii/S0001706X14003593 |
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ftunivesp:oai:acervodigital.unesp.br:11449/129375 2023-05-15T15:50:40+02:00 Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil Baum, Mauricio Castro, Edilene Alcantara de Pinto, Mara Cristina Goulart, Thais Marchi Baura, Walter Klisiowicz, Debora do Rocio Costa-Ribeiro, Magda Clara Vieira da Universidade Estadual Paulista (UNESP) 2015-10-21T20:57:33Z http://acervodigital.unesp.br/handle/11449/129375 http://hdl.handle.net/11449/129375 https://doi.org/10.1016/j.actatropica.2014.11.006 http://www.sciencedirect.com/science/article/pii/S0001706X14003593 eng eng Elsevier B.V. Acta Tropica Acta Tropica. Amsterdam: Elsevier Science Bv, v. 143, p. 8-12, 2015. 0001-706X http://hdl.handle.net/11449/129375 http://acervodigital.unesp.br/handle/11449/129375 http://dx.doi.org/10.1016/j.actatropica.2014.11.006 WOS:000350526800002 http://www.sciencedirect.com/science/article/pii/S0001706X14003593 info:eu-repo/semantics/closedAccess American cutaneous leishmaniasis Sand flies Prepronociceptin (PNOC) Host Reservoir outro 2015 ftunivesp https://doi.org/10.1016/j.actatropica.2014.11.006 2021-07-18T09:07:21Z The feeding behavior of sand flies provides valuable information about the vector/host interactions and elucidates the epidemiological patterns of American cutaneous leishmaniasis (ACL) transmission. The aim of this study was to identify the blood meal sources of sand flies in endemic areas of leishmaniasis in Parana State through polymerase chain reaction (PCR) amplification of a prepronociceptin (PNOC) gene fragment and its subsequent DNA sequencing. Moreover, molecular assays were conducted to evaluate the sensitivity and reproducibility of the PNOC gene amplification. Besides that, a time-course digestion test of the blood using sand flies that fed artificially on BALB/c mice was performed. Of 1263 female sand flies collected in the field, 93 (3.6%) specimens were engorged and 27 allowed efficient amplification of the PNOC gene. These flies had fed on equine (Equus caballus), porcine (Sus scrofa) and canine (Canis lupus familiaris) species. The results also showed that the identification of the blood meal sources of the sand flies using the molecular method was directly linked to the level of digestion of the blood (time-course) and not to the amount of blood that had been ingested or to the presence of inhibitors in the blood. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Other/Unknown Material Canis lupus Universidade Estadual Paulista São Paulo: Acervo Digital da UNESP / São Paulo State University Acta Tropica 143 8 12 |
institution |
Open Polar |
collection |
Universidade Estadual Paulista São Paulo: Acervo Digital da UNESP / São Paulo State University |
op_collection_id |
ftunivesp |
language |
English |
topic |
American cutaneous leishmaniasis Sand flies Prepronociceptin (PNOC) Host Reservoir |
spellingShingle |
American cutaneous leishmaniasis Sand flies Prepronociceptin (PNOC) Host Reservoir Baum, Mauricio Castro, Edilene Alcantara de Pinto, Mara Cristina Goulart, Thais Marchi Baura, Walter Klisiowicz, Debora do Rocio Costa-Ribeiro, Magda Clara Vieira da Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
topic_facet |
American cutaneous leishmaniasis Sand flies Prepronociceptin (PNOC) Host Reservoir |
description |
The feeding behavior of sand flies provides valuable information about the vector/host interactions and elucidates the epidemiological patterns of American cutaneous leishmaniasis (ACL) transmission. The aim of this study was to identify the blood meal sources of sand flies in endemic areas of leishmaniasis in Parana State through polymerase chain reaction (PCR) amplification of a prepronociceptin (PNOC) gene fragment and its subsequent DNA sequencing. Moreover, molecular assays were conducted to evaluate the sensitivity and reproducibility of the PNOC gene amplification. Besides that, a time-course digestion test of the blood using sand flies that fed artificially on BALB/c mice was performed. Of 1263 female sand flies collected in the field, 93 (3.6%) specimens were engorged and 27 allowed efficient amplification of the PNOC gene. These flies had fed on equine (Equus caballus), porcine (Sus scrofa) and canine (Canis lupus familiaris) species. The results also showed that the identification of the blood meal sources of the sand flies using the molecular method was directly linked to the level of digestion of the blood (time-course) and not to the amount of blood that had been ingested or to the presence of inhibitors in the blood. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Other/Unknown Material |
author |
Baum, Mauricio Castro, Edilene Alcantara de Pinto, Mara Cristina Goulart, Thais Marchi Baura, Walter Klisiowicz, Debora do Rocio Costa-Ribeiro, Magda Clara Vieira da |
author_facet |
Baum, Mauricio Castro, Edilene Alcantara de Pinto, Mara Cristina Goulart, Thais Marchi Baura, Walter Klisiowicz, Debora do Rocio Costa-Ribeiro, Magda Clara Vieira da |
author_sort |
Baum, Mauricio |
title |
Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
title_short |
Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
title_full |
Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
title_fullStr |
Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
title_full_unstemmed |
Molecular detection of the blood meal source of sand flies (Diptera: Psychodidae) in a transmission area of American cutaneous leishmaniasis, Parana State, Brazil |
title_sort |
molecular detection of the blood meal source of sand flies (diptera: psychodidae) in a transmission area of american cutaneous leishmaniasis, parana state, brazil |
publisher |
Elsevier B.V. |
publishDate |
2015 |
url |
http://acervodigital.unesp.br/handle/11449/129375 http://hdl.handle.net/11449/129375 https://doi.org/10.1016/j.actatropica.2014.11.006 http://www.sciencedirect.com/science/article/pii/S0001706X14003593 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
Acta Tropica Acta Tropica. Amsterdam: Elsevier Science Bv, v. 143, p. 8-12, 2015. 0001-706X http://hdl.handle.net/11449/129375 http://acervodigital.unesp.br/handle/11449/129375 http://dx.doi.org/10.1016/j.actatropica.2014.11.006 WOS:000350526800002 http://www.sciencedirect.com/science/article/pii/S0001706X14003593 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.actatropica.2014.11.006 |
container_title |
Acta Tropica |
container_volume |
143 |
container_start_page |
8 |
op_container_end_page |
12 |
_version_ |
1766385669397020672 |