d- and l-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method
Amino acids represent a fraction of organic matter in marine and freshwater ecosystems, and a source of carbon, nitrogen and energy. l-Amino acids are the most common enantiomers in nature because these chiral forms are used during the biosynthesis of proteins and peptide. To the contrary, the occur...
Published in: | Analytical and Bioanalytical Chemistry |
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Online Access: | http://hdl.handle.net/10278/43249 https://doi.org/10.1007/s00216-014-7961-y |
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ftuniveneziairis:oai:iris.unive.it:10278/43249 2024-04-14T08:02:54+00:00 d- and l-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method BARBARO, ELENA Zangrando, Roberta VECCHIATO, MARCO Turetta, Clara BARBANTE, Carlo GAMBARO, Andrea Barbaro, Elena Zangrando, Roberta Vecchiato, Marco Turetta, Clara Barbante, Carlo Gambaro, Andrea 2014 http://hdl.handle.net/10278/43249 https://doi.org/10.1007/s00216-014-7961-y eng eng info:eu-repo/semantics/altIdentifier/pmid/24961637 info:eu-repo/semantics/altIdentifier/wos/WOS:000340877200005 volume:406 issue:22 firstpage:5259 lastpage:5270 numberofpages:12 journal:ANALYTICAL AND BIOANALYTICAL CHEMISTRY http://hdl.handle.net/10278/43249 doi:10.1007/s00216-014-7961-y info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84907600356 info:eu-repo/semantics/openAccess Amino acids Chiral separation Antarctica HPLC-MS/MS HPLC-orbitrap Lakes NORTHERN VICTORIA-LAND CHIRAL STATIONARY-PHASE PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY DISSOLVED ORGANIC NITROGEN MCMURDO DRY VALLEYS TERRA-NOVA BAY WATER SEPARATION ECOSYSTEMS Settore CHIM/01 - Chimica Analitica info:eu-repo/semantics/article 2014 ftuniveneziairis https://doi.org/10.1007/s00216-014-7961-y 2024-03-21T18:20:11Z Amino acids represent a fraction of organic matter in marine and freshwater ecosystems, and a source of carbon, nitrogen and energy. l-Amino acids are the most common enantiomers in nature because these chiral forms are used during the biosynthesis of proteins and peptide. To the contrary, the occurrence of d-amino acids is usually linked to the presence of bacteria. We investigated the distribution of l- and d-amino acids in the lacustrine environment of Terra Nova Bay, Antarctica, in order to define their natural composition in this area and to individuate a possible relationship with primary production. A simultaneous chromatographic separation of 40 l- and d-amino acids was performed using a chiral stationary phase based on teicoplainin aglycone (chirobiotic tag). The chromatographic separation was coupled to two different mass spectrometers-an LTQ-Orbitrap XL (Thermo Fisher Scientific) and an API 4000 (ABSciex)-in order to investigate their quantitative performance. High-performance liquid chromatography coupled with mass spectrometry methods were evaluated through the estimation of their linear ranges, repeatability, accuracy and detection and quantification limits. The high-resolution mass spectrometer LTQ-Orbitrap XL presented detection limits between 0.4 and 7 mu g l (-1), while the triple quadrupole mass spectrometer API 4000 achieved the best detection limits reported in the literature for the quantification of amino acids (between 4 and 200 ng l (-1)). The most sensitive method, HPLC-API 4000, was applied to lake water samples. Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Dry Valleys Victoria Land Università Ca’ Foscari Venezia: ARCA (Archivio Istituzionale della Ricerca) Antarctic Victoria Land McMurdo Dry Valleys Terra Nova Bay Analytical and Bioanalytical Chemistry 406 22 5259 5270 |
institution |
Open Polar |
collection |
Università Ca’ Foscari Venezia: ARCA (Archivio Istituzionale della Ricerca) |
op_collection_id |
ftuniveneziairis |
language |
English |
topic |
Amino acids Chiral separation Antarctica HPLC-MS/MS HPLC-orbitrap Lakes NORTHERN VICTORIA-LAND CHIRAL STATIONARY-PHASE PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY DISSOLVED ORGANIC NITROGEN MCMURDO DRY VALLEYS TERRA-NOVA BAY WATER SEPARATION ECOSYSTEMS Settore CHIM/01 - Chimica Analitica |
spellingShingle |
Amino acids Chiral separation Antarctica HPLC-MS/MS HPLC-orbitrap Lakes NORTHERN VICTORIA-LAND CHIRAL STATIONARY-PHASE PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY DISSOLVED ORGANIC NITROGEN MCMURDO DRY VALLEYS TERRA-NOVA BAY WATER SEPARATION ECOSYSTEMS Settore CHIM/01 - Chimica Analitica BARBARO, ELENA Zangrando, Roberta VECCHIATO, MARCO Turetta, Clara BARBANTE, Carlo GAMBARO, Andrea d- and l-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method |
topic_facet |
Amino acids Chiral separation Antarctica HPLC-MS/MS HPLC-orbitrap Lakes NORTHERN VICTORIA-LAND CHIRAL STATIONARY-PHASE PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY DISSOLVED ORGANIC NITROGEN MCMURDO DRY VALLEYS TERRA-NOVA BAY WATER SEPARATION ECOSYSTEMS Settore CHIM/01 - Chimica Analitica |
description |
Amino acids represent a fraction of organic matter in marine and freshwater ecosystems, and a source of carbon, nitrogen and energy. l-Amino acids are the most common enantiomers in nature because these chiral forms are used during the biosynthesis of proteins and peptide. To the contrary, the occurrence of d-amino acids is usually linked to the presence of bacteria. We investigated the distribution of l- and d-amino acids in the lacustrine environment of Terra Nova Bay, Antarctica, in order to define their natural composition in this area and to individuate a possible relationship with primary production. A simultaneous chromatographic separation of 40 l- and d-amino acids was performed using a chiral stationary phase based on teicoplainin aglycone (chirobiotic tag). The chromatographic separation was coupled to two different mass spectrometers-an LTQ-Orbitrap XL (Thermo Fisher Scientific) and an API 4000 (ABSciex)-in order to investigate their quantitative performance. High-performance liquid chromatography coupled with mass spectrometry methods were evaluated through the estimation of their linear ranges, repeatability, accuracy and detection and quantification limits. The high-resolution mass spectrometer LTQ-Orbitrap XL presented detection limits between 0.4 and 7 mu g l (-1), while the triple quadrupole mass spectrometer API 4000 achieved the best detection limits reported in the literature for the quantification of amino acids (between 4 and 200 ng l (-1)). The most sensitive method, HPLC-API 4000, was applied to lake water samples. |
author2 |
Barbaro, Elena Zangrando, Roberta Vecchiato, Marco Turetta, Clara Barbante, Carlo Gambaro, Andrea |
format |
Article in Journal/Newspaper |
author |
BARBARO, ELENA Zangrando, Roberta VECCHIATO, MARCO Turetta, Clara BARBANTE, Carlo GAMBARO, Andrea |
author_facet |
BARBARO, ELENA Zangrando, Roberta VECCHIATO, MARCO Turetta, Clara BARBANTE, Carlo GAMBARO, Andrea |
author_sort |
BARBARO, ELENA |
title |
d- and l-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method |
title_short |
d- and l-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method |
title_full |
d- and l-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method |
title_fullStr |
d- and l-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method |
title_full_unstemmed |
d- and l-amino acids in Antarctic lakes: assessment of a very sensitive HPLC-MS method |
title_sort |
d- and l-amino acids in antarctic lakes: assessment of a very sensitive hplc-ms method |
publishDate |
2014 |
url |
http://hdl.handle.net/10278/43249 https://doi.org/10.1007/s00216-014-7961-y |
geographic |
Antarctic Victoria Land McMurdo Dry Valleys Terra Nova Bay |
geographic_facet |
Antarctic Victoria Land McMurdo Dry Valleys Terra Nova Bay |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys Victoria Land |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys Victoria Land |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/24961637 info:eu-repo/semantics/altIdentifier/wos/WOS:000340877200005 volume:406 issue:22 firstpage:5259 lastpage:5270 numberofpages:12 journal:ANALYTICAL AND BIOANALYTICAL CHEMISTRY http://hdl.handle.net/10278/43249 doi:10.1007/s00216-014-7961-y info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84907600356 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00216-014-7961-y |
container_title |
Analytical and Bioanalytical Chemistry |
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406 |
container_issue |
22 |
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5259 |
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5270 |
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