Stability and activity of enzyme aggregates of Calb in supercritical CO2
The activity and stability of a cross-linked enzyme aggregate of Candida antarctica lipase B (CLEA-Calb) in supercrit. carbon dioxide (SC-CO2) have been studied. The model reaction used is the esterification of isoamyl alc. with acetic acid. The catalytic performance of CLEA-Calb is evaluated both i...
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ftuniveindhoven:oai:library.tue.nl:640836 2023-05-15T13:56:31+02:00 Stability and activity of enzyme aggregates of Calb in supercritical CO2 Dijkstra, ZJ Zwannet Merchant, RZ Keurentjes, JTF Jos 2007 application/pdf http://repository.tue.nl/640836 en eng Copyright (c) Dijkstra, ZJ Zwannet Copyright (c) Merchant, RZ Copyright (c) Keurentjes, JTF Jos ISSN:0896-8446 Article / Letter to the editor 2007 ftuniveindhoven 2018-12-26T13:34:47Z The activity and stability of a cross-linked enzyme aggregate of Candida antarctica lipase B (CLEA-Calb) in supercrit. carbon dioxide (SC-CO2) have been studied. The model reaction used is the esterification of isoamyl alc. with acetic acid. The catalytic performance of CLEA-Calb is evaluated both in batch and in continuous expts., with a focus on the effect of water prodn. upon reaction. The results of the batch expts. show a decreasing initial activity of the CLEA-Calb with an increase in pressure. Moreover, CLEAs appear to be highly stable in supercrit. carbon dioxide and also remain active during continuous reactions. Suggestions for improved process designs are given for which the optimal design depends on the specific reaction requirements. Article in Journal/Newspaper Antarc* Antarctica Eindhoven University of Technology (TU/e): Research Portal |
institution |
Open Polar |
collection |
Eindhoven University of Technology (TU/e): Research Portal |
op_collection_id |
ftuniveindhoven |
language |
English |
description |
The activity and stability of a cross-linked enzyme aggregate of Candida antarctica lipase B (CLEA-Calb) in supercrit. carbon dioxide (SC-CO2) have been studied. The model reaction used is the esterification of isoamyl alc. with acetic acid. The catalytic performance of CLEA-Calb is evaluated both in batch and in continuous expts., with a focus on the effect of water prodn. upon reaction. The results of the batch expts. show a decreasing initial activity of the CLEA-Calb with an increase in pressure. Moreover, CLEAs appear to be highly stable in supercrit. carbon dioxide and also remain active during continuous reactions. Suggestions for improved process designs are given for which the optimal design depends on the specific reaction requirements. |
format |
Article in Journal/Newspaper |
author |
Dijkstra, ZJ Zwannet Merchant, RZ Keurentjes, JTF Jos |
spellingShingle |
Dijkstra, ZJ Zwannet Merchant, RZ Keurentjes, JTF Jos Stability and activity of enzyme aggregates of Calb in supercritical CO2 |
author_facet |
Dijkstra, ZJ Zwannet Merchant, RZ Keurentjes, JTF Jos |
author_sort |
Dijkstra, ZJ Zwannet |
title |
Stability and activity of enzyme aggregates of Calb in supercritical CO2 |
title_short |
Stability and activity of enzyme aggregates of Calb in supercritical CO2 |
title_full |
Stability and activity of enzyme aggregates of Calb in supercritical CO2 |
title_fullStr |
Stability and activity of enzyme aggregates of Calb in supercritical CO2 |
title_full_unstemmed |
Stability and activity of enzyme aggregates of Calb in supercritical CO2 |
title_sort |
stability and activity of enzyme aggregates of calb in supercritical co2 |
publishDate |
2007 |
url |
http://repository.tue.nl/640836 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ISSN:0896-8446 |
op_rights |
Copyright (c) Dijkstra, ZJ Zwannet Copyright (c) Merchant, RZ Copyright (c) Keurentjes, JTF Jos |
_version_ |
1766264023527981056 |