Modification of CCMV nanocages for enzyme encapsulation
In cellular systems, compartmentalization plays an important role in the protection and regulation of enzymes. Controlled encapsulation of enzymes in nanocompartments is crucial in understanding biocatalytic processes in the cellular environment. We have recently described an enzymatic method to cov...
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ftuniveindcris:oai:pure.tue.nl:publications/a8a802fb-e2c4-4247-80ec-6599bf8a36e3 2023-05-15T13:55:09+02:00 Modification of CCMV nanocages for enzyme encapsulation Schoonen, Lise van Hest, Jan C.M. Udit, A.K. 2018-06-05 https://research.tue.nl/en/publications/a8a802fb-e2c4-4247-80ec-6599bf8a36e3 https://doi.org/10.1007/978-1-4939-7893-9_6 http://www.scopus.com/inward/record.url?scp=85048174233&partnerID=8YFLogxK eng eng Springer info:eu-repo/semantics/closedAccess Schoonen , L & van Hest , J C M 2018 , Modification of CCMV nanocages for enzyme encapsulation . in A K Udit (ed.) , Protein Scaffolds : Design, Synthesis, and Applications . Methods in Molecular Biology , vol. 1798 , Springer , Dordrecht , pp. 69-83 . https://doi.org/10.1007/978-1-4939-7893-9_6 Candida antarctica lipase B Cowpea chlorotic mottle virus Esterase activity Self-assembly Sortagging Sortase A Viral capsid Gene Expression Models Molecular Aminoacyltransferases/chemistry Virus Assembly Bromovirus Capsid Proteins/chemistry Nanocapsules/chemistry Chromatography Affinity Enzymes Immobilized/chemistry Capsid/chemistry Mass Spectrometry Protein Conformation Bacterial Proteins/chemistry Cysteine Endopeptidases/chemistry Recombinant Fusion Proteins/chemistry Hydrogen-Ion Concentration bookPart 2018 ftuniveindcris https://doi.org/10.1007/978-1-4939-7893-9_6 2022-03-25T14:16:56Z In cellular systems, compartmentalization plays an important role in the protection and regulation of enzymes. Controlled encapsulation of enzymes in nanocompartments is crucial in understanding biocatalytic processes in the cellular environment. We have recently described an enzymatic method to covalently attach enzymes, equipped with a small recognition peptide, to the interior of viral capsids. Viral capsids are especially interesting in this respect, as they form very well-defined nanoparticles with a uniform size and shape. Here, we describe the relevant experimental procedures to encapsulate a model enzyme into the interior of a viral capsid, purify the resulting viral capsids, and measure the catalytic activity of the encapsulated enzymes. Book Part Antarc* Antarctica Eindhoven University of Technology research portal 69 83 |
institution |
Open Polar |
collection |
Eindhoven University of Technology research portal |
op_collection_id |
ftuniveindcris |
language |
English |
topic |
Candida antarctica lipase B Cowpea chlorotic mottle virus Esterase activity Self-assembly Sortagging Sortase A Viral capsid Gene Expression Models Molecular Aminoacyltransferases/chemistry Virus Assembly Bromovirus Capsid Proteins/chemistry Nanocapsules/chemistry Chromatography Affinity Enzymes Immobilized/chemistry Capsid/chemistry Mass Spectrometry Protein Conformation Bacterial Proteins/chemistry Cysteine Endopeptidases/chemistry Recombinant Fusion Proteins/chemistry Hydrogen-Ion Concentration |
spellingShingle |
Candida antarctica lipase B Cowpea chlorotic mottle virus Esterase activity Self-assembly Sortagging Sortase A Viral capsid Gene Expression Models Molecular Aminoacyltransferases/chemistry Virus Assembly Bromovirus Capsid Proteins/chemistry Nanocapsules/chemistry Chromatography Affinity Enzymes Immobilized/chemistry Capsid/chemistry Mass Spectrometry Protein Conformation Bacterial Proteins/chemistry Cysteine Endopeptidases/chemistry Recombinant Fusion Proteins/chemistry Hydrogen-Ion Concentration Schoonen, Lise van Hest, Jan C.M. Modification of CCMV nanocages for enzyme encapsulation |
topic_facet |
Candida antarctica lipase B Cowpea chlorotic mottle virus Esterase activity Self-assembly Sortagging Sortase A Viral capsid Gene Expression Models Molecular Aminoacyltransferases/chemistry Virus Assembly Bromovirus Capsid Proteins/chemistry Nanocapsules/chemistry Chromatography Affinity Enzymes Immobilized/chemistry Capsid/chemistry Mass Spectrometry Protein Conformation Bacterial Proteins/chemistry Cysteine Endopeptidases/chemistry Recombinant Fusion Proteins/chemistry Hydrogen-Ion Concentration |
description |
In cellular systems, compartmentalization plays an important role in the protection and regulation of enzymes. Controlled encapsulation of enzymes in nanocompartments is crucial in understanding biocatalytic processes in the cellular environment. We have recently described an enzymatic method to covalently attach enzymes, equipped with a small recognition peptide, to the interior of viral capsids. Viral capsids are especially interesting in this respect, as they form very well-defined nanoparticles with a uniform size and shape. Here, we describe the relevant experimental procedures to encapsulate a model enzyme into the interior of a viral capsid, purify the resulting viral capsids, and measure the catalytic activity of the encapsulated enzymes. |
author2 |
Udit, A.K. |
format |
Book Part |
author |
Schoonen, Lise van Hest, Jan C.M. |
author_facet |
Schoonen, Lise van Hest, Jan C.M. |
author_sort |
Schoonen, Lise |
title |
Modification of CCMV nanocages for enzyme encapsulation |
title_short |
Modification of CCMV nanocages for enzyme encapsulation |
title_full |
Modification of CCMV nanocages for enzyme encapsulation |
title_fullStr |
Modification of CCMV nanocages for enzyme encapsulation |
title_full_unstemmed |
Modification of CCMV nanocages for enzyme encapsulation |
title_sort |
modification of ccmv nanocages for enzyme encapsulation |
publisher |
Springer |
publishDate |
2018 |
url |
https://research.tue.nl/en/publications/a8a802fb-e2c4-4247-80ec-6599bf8a36e3 https://doi.org/10.1007/978-1-4939-7893-9_6 http://www.scopus.com/inward/record.url?scp=85048174233&partnerID=8YFLogxK |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Schoonen , L & van Hest , J C M 2018 , Modification of CCMV nanocages for enzyme encapsulation . in A K Udit (ed.) , Protein Scaffolds : Design, Synthesis, and Applications . Methods in Molecular Biology , vol. 1798 , Springer , Dordrecht , pp. 69-83 . https://doi.org/10.1007/978-1-4939-7893-9_6 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/978-1-4939-7893-9_6 |
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69 |
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83 |
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1766261419057086464 |