Anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan
Cereal grain-derived protein hydrolysates exert beneficial effects for human health, thus utilization of by-products with high protein content has drawn attention. In this study, hot water extraction of rice husk has been optimized to obtain protein hydrolysate with the highest anticancer activity....
Published in: | Journal of Industrial and Engineering Chemistry |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier Science Inc
2021
|
Subjects: | |
Online Access: | https://hdl.handle.net/11454/76667 https://doi.org/10.1016/j.jiec.2021.08.006 |
id |
ftunivege:oai:gcris.ege.edu.tr:11454/76667 |
---|---|
record_format |
openpolar |
spelling |
ftunivege:oai:gcris.ege.edu.tr:11454/76667 2023-12-17T10:49:36+01:00 Anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan Budak, Gozde Ilhan-Ayisigi, Esra Sevimli-Gur, Canan Yesil-Celiktas, Ozlem Celiktas, Melih Soner 2021 https://hdl.handle.net/11454/76667 https://doi.org/10.1016/j.jiec.2021.08.006 en eng Elsevier Science Inc Journal Of Industrial And Engineering Chemistry Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı 1876-794X 1226-086X https://hdl.handle.net/11454/76667 https://doi.org/10.1016/j.jiec.2021.08.006 doi:10.1016/j.jiec.2021.08.006 Budak, Gozde/0000-0001-5796-7202 CELIKTAS, Melih Soner Soner/0000-0003-0597-5133 Ilhan Ayisigi, Esra/0000-0003-1880-4261 103 381 391 none Rice husk Chitosan nanopArticle Water Extraction Protein Profile NanopArticles Cancer Antioxidant Silica Bran Peptide Hot water extraction Cytotoxicity Article 2021 ftunivege https://doi.org/10.1016/j.jiec.2021.08.006 2023-11-23T19:38:00Z Cereal grain-derived protein hydrolysates exert beneficial effects for human health, thus utilization of by-products with high protein content has drawn attention. In this study, hot water extraction of rice husk has been optimized to obtain protein hydrolysate with the highest anticancer activity. The optimum protein value was obtained as 2.40 g/L corresponding to 43.2 g protein/kg dried rice husk under the conditions of 60 degrees C, 2.0 mL/min flow rate and pH 10.0. The protein hydrolysate was encapsulated with chitosan, where the mean pArticle size of protein hydrolysate (0.3 %) loaded chitosan nanopArticles was 256.4 +/- 33. 4 nm with 89 % encapsulation efficiency and a 65% release at the end of 6 days. Cytotoxicity assays showed that the lowest cell viabilities have been achieved with A549 and MCF7 cells with IC50 values of 1.98 and 3.58 mu g/mL, respectively, whereas nuclear fragmentation and apoptotic bodies were observed through Hoechst 33342 staining. The cytotoxic effect might be associated with the wide variety of peptide/protein subunits ranging from 10 kDa to more than 180 kDa in the protein hydrolysate of rice husk. (C) 2021 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved. The research funds provided by Kocaeli University (KOU-BAP-2016-081 and KOU-BAP-2018-149) are highly appreciated. Special thanks to Mr. Ahmet Yilmaz who provided the rice husk from Ozserhat Gida Sanayi Tic. Ltd. Sti, Edirne, Turkey. We are grateful to Dilan Yerli for helping with SDS-PAGE, Prof. Dr. Kemal Sami Korkmaz for providing access to his lab at Ege University and Pelin Saglam-Metiner for her contributions to fluorescent staining assays. Kocaeli University [KOU-BAP-2016-081, KOU-BAP-2018-149] Article in Journal/Newspaper sami Ege University Institutional Repository Ege ENVELOPE(-55.853,-55.853,-83.560,-83.560) Journal of Industrial and Engineering Chemistry 103 381 391 |
institution |
Open Polar |
collection |
Ege University Institutional Repository |
op_collection_id |
ftunivege |
language |
English |
topic |
Rice husk Chitosan nanopArticle Water Extraction Protein Profile NanopArticles Cancer Antioxidant Silica Bran Peptide Hot water extraction Cytotoxicity |
spellingShingle |
Rice husk Chitosan nanopArticle Water Extraction Protein Profile NanopArticles Cancer Antioxidant Silica Bran Peptide Hot water extraction Cytotoxicity Budak, Gozde Ilhan-Ayisigi, Esra Sevimli-Gur, Canan Yesil-Celiktas, Ozlem Celiktas, Melih Soner Anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan |
topic_facet |
Rice husk Chitosan nanopArticle Water Extraction Protein Profile NanopArticles Cancer Antioxidant Silica Bran Peptide Hot water extraction Cytotoxicity |
description |
Cereal grain-derived protein hydrolysates exert beneficial effects for human health, thus utilization of by-products with high protein content has drawn attention. In this study, hot water extraction of rice husk has been optimized to obtain protein hydrolysate with the highest anticancer activity. The optimum protein value was obtained as 2.40 g/L corresponding to 43.2 g protein/kg dried rice husk under the conditions of 60 degrees C, 2.0 mL/min flow rate and pH 10.0. The protein hydrolysate was encapsulated with chitosan, where the mean pArticle size of protein hydrolysate (0.3 %) loaded chitosan nanopArticles was 256.4 +/- 33. 4 nm with 89 % encapsulation efficiency and a 65% release at the end of 6 days. Cytotoxicity assays showed that the lowest cell viabilities have been achieved with A549 and MCF7 cells with IC50 values of 1.98 and 3.58 mu g/mL, respectively, whereas nuclear fragmentation and apoptotic bodies were observed through Hoechst 33342 staining. The cytotoxic effect might be associated with the wide variety of peptide/protein subunits ranging from 10 kDa to more than 180 kDa in the protein hydrolysate of rice husk. (C) 2021 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved. The research funds provided by Kocaeli University (KOU-BAP-2016-081 and KOU-BAP-2018-149) are highly appreciated. Special thanks to Mr. Ahmet Yilmaz who provided the rice husk from Ozserhat Gida Sanayi Tic. Ltd. Sti, Edirne, Turkey. We are grateful to Dilan Yerli for helping with SDS-PAGE, Prof. Dr. Kemal Sami Korkmaz for providing access to his lab at Ege University and Pelin Saglam-Metiner for her contributions to fluorescent staining assays. Kocaeli University [KOU-BAP-2016-081, KOU-BAP-2018-149] |
format |
Article in Journal/Newspaper |
author |
Budak, Gozde Ilhan-Ayisigi, Esra Sevimli-Gur, Canan Yesil-Celiktas, Ozlem Celiktas, Melih Soner |
author_facet |
Budak, Gozde Ilhan-Ayisigi, Esra Sevimli-Gur, Canan Yesil-Celiktas, Ozlem Celiktas, Melih Soner |
author_sort |
Budak, Gozde |
title |
Anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan |
title_short |
Anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan |
title_full |
Anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan |
title_fullStr |
Anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan |
title_full_unstemmed |
Anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan |
title_sort |
anticancer activities of bioactive peptides derived from rice husk both in free and encapsulated form in chitosan |
publisher |
Elsevier Science Inc |
publishDate |
2021 |
url |
https://hdl.handle.net/11454/76667 https://doi.org/10.1016/j.jiec.2021.08.006 |
long_lat |
ENVELOPE(-55.853,-55.853,-83.560,-83.560) |
geographic |
Ege |
geographic_facet |
Ege |
genre |
sami |
genre_facet |
sami |
op_relation |
Journal Of Industrial And Engineering Chemistry Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı 1876-794X 1226-086X https://hdl.handle.net/11454/76667 https://doi.org/10.1016/j.jiec.2021.08.006 doi:10.1016/j.jiec.2021.08.006 Budak, Gozde/0000-0001-5796-7202 CELIKTAS, Melih Soner Soner/0000-0003-0597-5133 Ilhan Ayisigi, Esra/0000-0003-1880-4261 103 381 391 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.jiec.2021.08.006 |
container_title |
Journal of Industrial and Engineering Chemistry |
container_volume |
103 |
container_start_page |
381 |
op_container_end_page |
391 |
_version_ |
1785574108580282368 |