Poly-3-hydroxybutyrate-producing extreme halophilic archaeon: Haloferax sp MA10 isolated from Camalti Saltern, Izmir

• Published in: Turkish Journal of Biology
• Main Authors: Ates, Mustafa, Kahraman, Ozge, Tekin, Ebru
• Format: Article in Journal/Newspaper
• Language: English
• Published: Tubitak Scientific & Technical Research Council Turkey 2012
• Subjects: poly-3-hydroxybutyrate (PHB); Extremely halophilic archaeon; PHB extraction and quantification; Haloferax sp MA 10; 16S rRNA gene; Ege; Saltern; sami
• Online Access: https://hdl.handle.net/11454/45560; https://doi.org/10.3906/biy-1102-16

WOS: 000304071800008 Izmir Camalti Saltern is the biggest seawater-based saltern in Turkey. To date, it has not been investigated extensively for the existence of poly-3-hydroxybutyrate (PHB)-producing species. In this study, 14 extremely halophilic archaea were isolated, purified, and screened for PHB production. One strain was then selected as the best PHB producer, further cultivated in different PHB media, and compared with a positive control, Haloferax mediterranei ATCC 33500. PHB was extracted from cells and measured with a spectrophotometer, and then the amount of PHB was measured through comparison with standard PHB. The detected high PHB yield was 6.53% of the dry cell weight in the PHB medium supplemented with acetate. Partial 16S rRNA gene sequence analysis showed 99% similarity to Haloferax alexandrinus strain TMT; therefore, the strain was named Haloferax sp. MA10. Haloferax sp. MA 10 and Haloferax alexandrinus TMT have some differences in phenotypic and biochemical properties. With this study, the discovery of PHB-producing extreme halophilic archaeon Haloferax sp. MA 10 at Izmir's Camalti Saltern is reported for the first time. Scientific Research Project Council of Ege UniversityEge University [FEN-045] This work was supported by the Scientific Research Project Council of Ege University, Project No. FEN-045, 2006. We thank the Scientific Research Project Council of Ege University. We also thank Dr Aziz Akin Denizci, Assoc Prof Dr Birgul Ozcan, and Assoc Prof Dr Kemal Sami Korkmaz.