Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species
Inward rectifier K+ (Kir2) channels are critical for electrical excitability of cardiac myocytes. Here, we examine expression of Kir2 channels in the heart of three Gadiformes species, polar cod (Boreogadus saida) and navaga (Eleginus nawaga) of the Arctic Ocean and burbot (Lota lota) of the tempera...
Published in: | Journal of Comparative Physiology B |
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Online Access: | https://erepo.uef.fi/handle/123456789/7872 |
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ftuniveasternfin:oai:erepo.uef.fi:123456789/7872 2023-05-15T14:54:46+02:00 Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species Hassinen, M Korajoki, H Abramochkin, D Krivosheya, P Vornanen, M Ympäristö- ja biotieteiden laitos / Toiminta 2019-12-13T12:53:10Z 735-749 https://erepo.uef.fi/handle/123456789/7872 englanti unknown Saksa Journal of comparative physiology B : biochemical, systemic, and environmental physiology http://dx.doi.org/10.1007/s00360-019-01241-9 10.1007/s00360-019-01241-9 0174-1578 6 189 https://erepo.uef.fi/handle/123456789/7872 CC BY 4.0 openAccess © Authors https://creativecommons.org/licenses/by/4.0/ CC-BY fish heart inward rectifier potassium channels Kir2 paralogues thermal acclimation RT-qPCR Tieteelliset aikakauslehtiartikkelit A1 article artikkeli 2019 ftuniveasternfin https://doi.org/10.1007/s00360-019-01241-9 2023-01-25T23:58:29Z Inward rectifier K+ (Kir2) channels are critical for electrical excitability of cardiac myocytes. Here, we examine expression of Kir2 channels in the heart of three Gadiformes species, polar cod (Boreogadus saida) and navaga (Eleginus nawaga) of the Arctic Ocean and burbot (Lota lota) of the temperate lakes to find out the role of Kir2 channels in cardiac adaptation to cold. Five boreal freshwater species: brown trout (Salmo trutta fario), arctic char (Salvelinus alpinus), roach (Rutilus rutilus), perch (Perca fluviatilis) and pike (Esox lucius), and zebrafish (Danio rerio), were included for comparison. Transcript expression of genes encoding Kir2.1a, − 2.1b, − 2.2a, − 2.2b and − 2.4 was studied from atrium and ventricle of thermally acclimated or acclimatized fish by quantitative PCR. Kir2 composition in the polar cod was more diverse than in other species in that all Kir2 isoforms were relatively highly expressed. Kir2 composition of navaga and burbot differed from that of the polar cod as well as from those of other species. The relative expression of Kir2.2 transcripts, especially Kir2.2b, was higher in both atrium and ventricle of navaga and burbot (56–89% from the total Kir2 pool) than in other species (0.1–11%). Thermal acclimation induced only small changes in cardiac Kir2 transcript expression in Gadiformes species. However, Kir2.2b transcripts were upregulated in cold-acclimated navaga and burbot hearts. All in all, the cardiac Kir2 composition seems to be dependent on both phylogenetic position and thermal preference of the fish. final draft peerReviewed Article in Journal/Newspaper Arctic Arctic Ocean Boreogadus saida Burbot Esox lucius Lota lota polar cod Salvelinus alpinus lota UEF eRepository (University of Eastern Finland) Arctic Arctic Ocean Navaga ENVELOPE(162.106,162.106,59.229,59.229) Journal of Comparative Physiology B 189 6 735 749 |
institution |
Open Polar |
collection |
UEF eRepository (University of Eastern Finland) |
op_collection_id |
ftuniveasternfin |
language |
unknown |
topic |
fish heart inward rectifier potassium channels Kir2 paralogues thermal acclimation RT-qPCR |
spellingShingle |
fish heart inward rectifier potassium channels Kir2 paralogues thermal acclimation RT-qPCR Hassinen, M Korajoki, H Abramochkin, D Krivosheya, P Vornanen, M Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
topic_facet |
fish heart inward rectifier potassium channels Kir2 paralogues thermal acclimation RT-qPCR |
description |
Inward rectifier K+ (Kir2) channels are critical for electrical excitability of cardiac myocytes. Here, we examine expression of Kir2 channels in the heart of three Gadiformes species, polar cod (Boreogadus saida) and navaga (Eleginus nawaga) of the Arctic Ocean and burbot (Lota lota) of the temperate lakes to find out the role of Kir2 channels in cardiac adaptation to cold. Five boreal freshwater species: brown trout (Salmo trutta fario), arctic char (Salvelinus alpinus), roach (Rutilus rutilus), perch (Perca fluviatilis) and pike (Esox lucius), and zebrafish (Danio rerio), were included for comparison. Transcript expression of genes encoding Kir2.1a, − 2.1b, − 2.2a, − 2.2b and − 2.4 was studied from atrium and ventricle of thermally acclimated or acclimatized fish by quantitative PCR. Kir2 composition in the polar cod was more diverse than in other species in that all Kir2 isoforms were relatively highly expressed. Kir2 composition of navaga and burbot differed from that of the polar cod as well as from those of other species. The relative expression of Kir2.2 transcripts, especially Kir2.2b, was higher in both atrium and ventricle of navaga and burbot (56–89% from the total Kir2 pool) than in other species (0.1–11%). Thermal acclimation induced only small changes in cardiac Kir2 transcript expression in Gadiformes species. However, Kir2.2b transcripts were upregulated in cold-acclimated navaga and burbot hearts. All in all, the cardiac Kir2 composition seems to be dependent on both phylogenetic position and thermal preference of the fish. final draft peerReviewed |
author2 |
Ympäristö- ja biotieteiden laitos / Toiminta |
format |
Article in Journal/Newspaper |
author |
Hassinen, M Korajoki, H Abramochkin, D Krivosheya, P Vornanen, M |
author_facet |
Hassinen, M Korajoki, H Abramochkin, D Krivosheya, P Vornanen, M |
author_sort |
Hassinen, M |
title |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_short |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_full |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_fullStr |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_full_unstemmed |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_sort |
transcript expression of inward rectifier potassium channels of kir2 subfamily in arctic marine and freshwater fish species |
publisher |
Saksa |
publishDate |
2019 |
url |
https://erepo.uef.fi/handle/123456789/7872 |
long_lat |
ENVELOPE(162.106,162.106,59.229,59.229) |
geographic |
Arctic Arctic Ocean Navaga |
geographic_facet |
Arctic Arctic Ocean Navaga |
genre |
Arctic Arctic Ocean Boreogadus saida Burbot Esox lucius Lota lota polar cod Salvelinus alpinus lota |
genre_facet |
Arctic Arctic Ocean Boreogadus saida Burbot Esox lucius Lota lota polar cod Salvelinus alpinus lota |
op_relation |
Journal of comparative physiology B : biochemical, systemic, and environmental physiology http://dx.doi.org/10.1007/s00360-019-01241-9 10.1007/s00360-019-01241-9 0174-1578 6 189 https://erepo.uef.fi/handle/123456789/7872 |
op_rights |
CC BY 4.0 openAccess © Authors https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00360-019-01241-9 |
container_title |
Journal of Comparative Physiology B |
container_volume |
189 |
container_issue |
6 |
container_start_page |
735 |
op_container_end_page |
749 |
_version_ |
1766326517768388608 |