Biosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by Antarctic Pseudomonas fragi

Previously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by the Antarctic strain Pseudomonas fragi GC01. Here we studied the role of volatile sulfur compounds (VSCs) in the biosynthesis of CdS QDs by P. fragi GC01. The biosynthesis of nanopa...

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Published in:Frontiers in Microbiology
Main Authors: Gallardo-Benavente, Carla, Carrión, Ornella, Todd, Jonathan D., Pieretti, Joana C., Seabra, Amedea B., Durán, Nelson, Rubilar, Olga, Pérez-Donoso, José M., Quiroz, Andrés
Format: Article in Journal/Newspaper
Language:English
Published: 2019
Subjects:
Online Access:https://ueaeprints.uea.ac.uk/id/eprint/72254/
https://ueaeprints.uea.ac.uk/id/eprint/72254/1/fmicb_10_01866.pdf
https://doi.org/10.3389/fmicb.2019.01866
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spelling ftuniveastangl:oai:ueaeprints.uea.ac.uk:72254 2023-05-15T14:00:22+02:00 Biosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by Antarctic Pseudomonas fragi Gallardo-Benavente, Carla Carrión, Ornella Todd, Jonathan D. Pieretti, Joana C. Seabra, Amedea B. Durán, Nelson Rubilar, Olga Pérez-Donoso, José M. Quiroz, Andrés 2019-08-13 application/pdf https://ueaeprints.uea.ac.uk/id/eprint/72254/ https://ueaeprints.uea.ac.uk/id/eprint/72254/1/fmicb_10_01866.pdf https://doi.org/10.3389/fmicb.2019.01866 en eng https://ueaeprints.uea.ac.uk/id/eprint/72254/1/fmicb_10_01866.pdf Gallardo-Benavente, Carla, Carrión, Ornella, Todd, Jonathan D., Pieretti, Joana C., Seabra, Amedea B., Durán, Nelson, Rubilar, Olga, Pérez-Donoso, José M. and Quiroz, Andrés (2019) Biosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by Antarctic Pseudomonas fragi. Frontiers in Microbiology, 10. ISSN 1664-302X doi:10.3389/fmicb.2019.01866 cc_by CC-BY Article PeerReviewed 2019 ftuniveastangl https://doi.org/10.3389/fmicb.2019.01866 2023-01-30T21:51:02Z Previously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by the Antarctic strain Pseudomonas fragi GC01. Here we studied the role of volatile sulfur compounds (VSCs) in the biosynthesis of CdS QDs by P. fragi GC01. The biosynthesis of nanoparticles was evaluated in the presence of sulfate, sulfite, thiosulfate, sulfide, cysteine and methionine as sole sulfur sources. Intracellular biosynthesis occurred with all sulfur sources tested. However, extracellular biosynthesis was observed only in cultures amended with cysteine (Cys) and methionine (Met). Extracellular nanoparticles were characterized by dynamic light scattering, absorption and emission spectra, energy dispersive X-ray, atomic force microscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Purified QDs correspond to cubic nanocrystals of CdS with sizes between 2 and 16 nm. The analysis of VSCs revealed that P. fragi GC01 produced hydrogen sulfide (H2S), methanethiol (MeSH) and dimethyl sulfide (DMS) in the presence of sulfate, Met or Cys. Dimethyl disulfide (DMDS) was only detected in the presence of Met. Interestingly, MeSH was the main VSC produced in this condition. In addition, MeSH was the only VSC for which the concentration decreased in the presence of cadmium (Cd) of all the sulfur sources tested, suggesting that this gas interacts with Cd to form nanoparticles. The role of MeSH and DMS on Cds QDs biosynthesis was evaluated in two mutants of the Antarctic strain Pseudomonas deceptionensis M1T: megL- (unable to produce MeSH from Met) and mddA- (unable to generate DMS from MeSH). No biosynthesis of QDs was observed in the megL- strain, confirming the importance of MeSH in QD biosynthesis. In addition, the production of QDs in the mddA- strain was not affected, indicating that DMS is not a substrate for the biosynthesis of nanoparticles. Here, we confirm a link between MeSH production and CdS QDs biosynthesis when Met is used as sole sulfur ... Article in Journal/Newspaper Antarc* Antarctic University of East Anglia: UEA Digital Repository Antarctic The Antarctic Frontiers in Microbiology 10
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language English
description Previously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by the Antarctic strain Pseudomonas fragi GC01. Here we studied the role of volatile sulfur compounds (VSCs) in the biosynthesis of CdS QDs by P. fragi GC01. The biosynthesis of nanoparticles was evaluated in the presence of sulfate, sulfite, thiosulfate, sulfide, cysteine and methionine as sole sulfur sources. Intracellular biosynthesis occurred with all sulfur sources tested. However, extracellular biosynthesis was observed only in cultures amended with cysteine (Cys) and methionine (Met). Extracellular nanoparticles were characterized by dynamic light scattering, absorption and emission spectra, energy dispersive X-ray, atomic force microscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Purified QDs correspond to cubic nanocrystals of CdS with sizes between 2 and 16 nm. The analysis of VSCs revealed that P. fragi GC01 produced hydrogen sulfide (H2S), methanethiol (MeSH) and dimethyl sulfide (DMS) in the presence of sulfate, Met or Cys. Dimethyl disulfide (DMDS) was only detected in the presence of Met. Interestingly, MeSH was the main VSC produced in this condition. In addition, MeSH was the only VSC for which the concentration decreased in the presence of cadmium (Cd) of all the sulfur sources tested, suggesting that this gas interacts with Cd to form nanoparticles. The role of MeSH and DMS on Cds QDs biosynthesis was evaluated in two mutants of the Antarctic strain Pseudomonas deceptionensis M1T: megL- (unable to produce MeSH from Met) and mddA- (unable to generate DMS from MeSH). No biosynthesis of QDs was observed in the megL- strain, confirming the importance of MeSH in QD biosynthesis. In addition, the production of QDs in the mddA- strain was not affected, indicating that DMS is not a substrate for the biosynthesis of nanoparticles. Here, we confirm a link between MeSH production and CdS QDs biosynthesis when Met is used as sole sulfur ...
format Article in Journal/Newspaper
author Gallardo-Benavente, Carla
Carrión, Ornella
Todd, Jonathan D.
Pieretti, Joana C.
Seabra, Amedea B.
Durán, Nelson
Rubilar, Olga
Pérez-Donoso, José M.
Quiroz, Andrés
spellingShingle Gallardo-Benavente, Carla
Carrión, Ornella
Todd, Jonathan D.
Pieretti, Joana C.
Seabra, Amedea B.
Durán, Nelson
Rubilar, Olga
Pérez-Donoso, José M.
Quiroz, Andrés
Biosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by Antarctic Pseudomonas fragi
author_facet Gallardo-Benavente, Carla
Carrión, Ornella
Todd, Jonathan D.
Pieretti, Joana C.
Seabra, Amedea B.
Durán, Nelson
Rubilar, Olga
Pérez-Donoso, José M.
Quiroz, Andrés
author_sort Gallardo-Benavente, Carla
title Biosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by Antarctic Pseudomonas fragi
title_short Biosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by Antarctic Pseudomonas fragi
title_full Biosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by Antarctic Pseudomonas fragi
title_fullStr Biosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by Antarctic Pseudomonas fragi
title_full_unstemmed Biosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by Antarctic Pseudomonas fragi
title_sort biosynthesis of cds quantum dots mediated by volatile sulfur compounds released by antarctic pseudomonas fragi
publishDate 2019
url https://ueaeprints.uea.ac.uk/id/eprint/72254/
https://ueaeprints.uea.ac.uk/id/eprint/72254/1/fmicb_10_01866.pdf
https://doi.org/10.3389/fmicb.2019.01866
geographic Antarctic
The Antarctic
geographic_facet Antarctic
The Antarctic
genre Antarc*
Antarctic
genre_facet Antarc*
Antarctic
op_relation https://ueaeprints.uea.ac.uk/id/eprint/72254/1/fmicb_10_01866.pdf
Gallardo-Benavente, Carla, Carrión, Ornella, Todd, Jonathan D., Pieretti, Joana C., Seabra, Amedea B., Durán, Nelson, Rubilar, Olga, Pérez-Donoso, José M. and Quiroz, Andrés (2019) Biosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by Antarctic Pseudomonas fragi. Frontiers in Microbiology, 10. ISSN 1664-302X
doi:10.3389/fmicb.2019.01866
op_rights cc_by
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fmicb.2019.01866
container_title Frontiers in Microbiology
container_volume 10
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