SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes
The accurate quantification of eukaryotic species abundances from bulk samples remains a key challenge for community ecology and environmental biomonitoring. We resolve this challenge by combining shotgun sequencing, mapping to reference DNA barcodes or to mitogenomes, and three correction factors:...
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2020
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Online Access: | https://ueaeprints.uea.ac.uk/id/eprint/71876/ https://ueaeprints.uea.ac.uk/id/eprint/71876/1/Accepted_Manuscript.pdf https://doi.org/10.1111/1755-0998.13057 |
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ftuniveastangl:oai:ueaeprints.uea.ac.uk:71876 2023-05-15T15:04:37+02:00 SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes Ji, Yinqiu Huotari, Tea Roslin, Tomas Schmidt, Niels Martin Wang, Jiaxin Yu, Douglas W. Ovaskainen, Otso 2020-01 application/pdf https://ueaeprints.uea.ac.uk/id/eprint/71876/ https://ueaeprints.uea.ac.uk/id/eprint/71876/1/Accepted_Manuscript.pdf https://doi.org/10.1111/1755-0998.13057 en eng https://ueaeprints.uea.ac.uk/id/eprint/71876/1/Accepted_Manuscript.pdf Ji, Yinqiu, Huotari, Tea, Roslin, Tomas, Schmidt, Niels Martin, Wang, Jiaxin, Yu, Douglas W. and Ovaskainen, Otso (2020) SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes. Molecular Ecology Resources, 20 (1). pp. 256-267. ISSN 1755-098X doi:10.1111/1755-0998.13057 Article PeerReviewed 2020 ftuniveastangl https://doi.org/10.1111/1755-0998.13057 2023-01-30T21:50:50Z The accurate quantification of eukaryotic species abundances from bulk samples remains a key challenge for community ecology and environmental biomonitoring. We resolve this challenge by combining shotgun sequencing, mapping to reference DNA barcodes or to mitogenomes, and three correction factors: (a) a percent-coverage threshold to filter out false positives, (b) an internal-standard DNA spike-in to correct for stochasticity during sequencing, and (c) technical replicates to correct for stochasticity across sequencing runs. The SPIKEPIPE pipeline achieves a strikingly high accuracy of intraspecific abundance estimates (in terms of DNA mass) from samples of known composition (mapping to barcodes R2 = .93, mitogenomes R2 = .95) and a high repeatability across environmental-sample replicates (barcodes R2 = .94, mitogenomes R2 = .93). As proof of concept, we sequence arthropod samples from the High Arctic, systematically collected over 17 years, detecting changes in species richness, species-specific abundances, and phenology. SPIKEPIPE provides cost-efficient and reliable quantification of eukaryotic communities. Article in Journal/Newspaper Arctic University of East Anglia: UEA Digital Repository Arctic Molecular Ecology Resources 20 1 256 267 |
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University of East Anglia: UEA Digital Repository |
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ftuniveastangl |
language |
English |
description |
The accurate quantification of eukaryotic species abundances from bulk samples remains a key challenge for community ecology and environmental biomonitoring. We resolve this challenge by combining shotgun sequencing, mapping to reference DNA barcodes or to mitogenomes, and three correction factors: (a) a percent-coverage threshold to filter out false positives, (b) an internal-standard DNA spike-in to correct for stochasticity during sequencing, and (c) technical replicates to correct for stochasticity across sequencing runs. The SPIKEPIPE pipeline achieves a strikingly high accuracy of intraspecific abundance estimates (in terms of DNA mass) from samples of known composition (mapping to barcodes R2 = .93, mitogenomes R2 = .95) and a high repeatability across environmental-sample replicates (barcodes R2 = .94, mitogenomes R2 = .93). As proof of concept, we sequence arthropod samples from the High Arctic, systematically collected over 17 years, detecting changes in species richness, species-specific abundances, and phenology. SPIKEPIPE provides cost-efficient and reliable quantification of eukaryotic communities. |
format |
Article in Journal/Newspaper |
author |
Ji, Yinqiu Huotari, Tea Roslin, Tomas Schmidt, Niels Martin Wang, Jiaxin Yu, Douglas W. Ovaskainen, Otso |
spellingShingle |
Ji, Yinqiu Huotari, Tea Roslin, Tomas Schmidt, Niels Martin Wang, Jiaxin Yu, Douglas W. Ovaskainen, Otso SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes |
author_facet |
Ji, Yinqiu Huotari, Tea Roslin, Tomas Schmidt, Niels Martin Wang, Jiaxin Yu, Douglas W. Ovaskainen, Otso |
author_sort |
Ji, Yinqiu |
title |
SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes |
title_short |
SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes |
title_full |
SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes |
title_fullStr |
SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes |
title_full_unstemmed |
SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes |
title_sort |
spikepipe: a metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using dna barcodes or mitogenomes |
publishDate |
2020 |
url |
https://ueaeprints.uea.ac.uk/id/eprint/71876/ https://ueaeprints.uea.ac.uk/id/eprint/71876/1/Accepted_Manuscript.pdf https://doi.org/10.1111/1755-0998.13057 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
https://ueaeprints.uea.ac.uk/id/eprint/71876/1/Accepted_Manuscript.pdf Ji, Yinqiu, Huotari, Tea, Roslin, Tomas, Schmidt, Niels Martin, Wang, Jiaxin, Yu, Douglas W. and Ovaskainen, Otso (2020) SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes. Molecular Ecology Resources, 20 (1). pp. 256-267. ISSN 1755-098X doi:10.1111/1755-0998.13057 |
op_doi |
https://doi.org/10.1111/1755-0998.13057 |
container_title |
Molecular Ecology Resources |
container_volume |
20 |
container_issue |
1 |
container_start_page |
256 |
op_container_end_page |
267 |
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1766336358040731648 |