N-acetylhistidine, a novel osmolyte in the lens of Atlantic salmon (Salmo salar L.)
Volume homeostasis is essential for the preservation of lens transparency and this is of particular significance to anadromous fish species where migration from freshwater to seawater presents severe osmotic challenges. In Atlantic salmon (Salmo salar L.), aqueous humor (AH) osmolality is greater in...
Published in: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
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ftuniveastangl:oai:ueaeprints.uea.ac.uk:28409 2023-05-15T15:31:52+02:00 N-acetylhistidine, a novel osmolyte in the lens of Atlantic salmon (Salmo salar L.) Rhodes, Jeremy D. Breck, Olav Waagbø, Rune Bjerkas, Ellen Sanderson, Julie 2010-10 application/pdf https://ueaeprints.uea.ac.uk/id/eprint/28409/ https://ueaeprints.uea.ac.uk/id/eprint/28409/1/Rhodes_et_al,_2010.pdf https://doi.org/10.1152/ajpregu.00214.2010 en eng https://ueaeprints.uea.ac.uk/id/eprint/28409/1/Rhodes_et_al,_2010.pdf Rhodes, Jeremy D., Breck, Olav, Waagbø, Rune, Bjerkas, Ellen and Sanderson, Julie (2010) N-acetylhistidine, a novel osmolyte in the lens of Atlantic salmon (Salmo salar L.). AJP - Regulatory, Integrative and Comparative Physiology, 299 (4). R1075-R1081. doi:10.1152/ajpregu.00214.2010 Article PeerReviewed 2010 ftuniveastangl https://doi.org/10.1152/ajpregu.00214.2010 2023-01-30T21:28:20Z Volume homeostasis is essential for the preservation of lens transparency and this is of particular significance to anadromous fish species where migration from freshwater to seawater presents severe osmotic challenges. In Atlantic salmon (Salmo salar L.), aqueous humor (AH) osmolality is greater in fish acclimated to seawater compared with young freshwater fish, and levels of lens N-acetylhistidine (NAH) are much higher in seawater fish. Here we investigate NAH as an osmolyte in the lenses of salmon receiving diets either with or without histidine supplementation. In the histidine-supplemented diet (HD) histidine content was 14.2 g/kg, and in the control diet (CD) histidine content was 8.9 g/kg. A transient increase in AH osmolality of 20 mmol/kg was observed in fish transferred from freshwater to seawater. In a lens culture model, temporary decreases in volume and transparency were observed when lenses were exposed to hyperosmotic conditions. A positive linear relationship between extracellular osmolality and lens NAH content was also observed, whereas there was no change in lens histidine content. Hypoosmotic exposure stimulated [14C]-histidine efflux by 9.2- and 2.6-fold in CD and HD lenses, respectively. NAH efflux, measured by HPLC, was stimulated by hypoosmotic exposure to a much greater extent in HD lenses. In vivo, lens NAH increased in response to elevated AH osmolality in HD but not CD fish. In conclusion, NAH has an important and novel role as a compatible osmolyte in salmon lens. Furthermore, it is the major osmolyte that balances increases in AH osmolality in NAH would lead to a dysfunction of the normal osmoregulatory processes in the lens, and we propose that this would contribute to cataract formation in fish deficient in histidine. Article in Journal/Newspaper Atlantic salmon Salmo salar University of East Anglia: UEA Digital Repository American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 299 4 R1075 R1081 |
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Open Polar |
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University of East Anglia: UEA Digital Repository |
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ftuniveastangl |
language |
English |
description |
Volume homeostasis is essential for the preservation of lens transparency and this is of particular significance to anadromous fish species where migration from freshwater to seawater presents severe osmotic challenges. In Atlantic salmon (Salmo salar L.), aqueous humor (AH) osmolality is greater in fish acclimated to seawater compared with young freshwater fish, and levels of lens N-acetylhistidine (NAH) are much higher in seawater fish. Here we investigate NAH as an osmolyte in the lenses of salmon receiving diets either with or without histidine supplementation. In the histidine-supplemented diet (HD) histidine content was 14.2 g/kg, and in the control diet (CD) histidine content was 8.9 g/kg. A transient increase in AH osmolality of 20 mmol/kg was observed in fish transferred from freshwater to seawater. In a lens culture model, temporary decreases in volume and transparency were observed when lenses were exposed to hyperosmotic conditions. A positive linear relationship between extracellular osmolality and lens NAH content was also observed, whereas there was no change in lens histidine content. Hypoosmotic exposure stimulated [14C]-histidine efflux by 9.2- and 2.6-fold in CD and HD lenses, respectively. NAH efflux, measured by HPLC, was stimulated by hypoosmotic exposure to a much greater extent in HD lenses. In vivo, lens NAH increased in response to elevated AH osmolality in HD but not CD fish. In conclusion, NAH has an important and novel role as a compatible osmolyte in salmon lens. Furthermore, it is the major osmolyte that balances increases in AH osmolality in NAH would lead to a dysfunction of the normal osmoregulatory processes in the lens, and we propose that this would contribute to cataract formation in fish deficient in histidine. |
format |
Article in Journal/Newspaper |
author |
Rhodes, Jeremy D. Breck, Olav Waagbø, Rune Bjerkas, Ellen Sanderson, Julie |
spellingShingle |
Rhodes, Jeremy D. Breck, Olav Waagbø, Rune Bjerkas, Ellen Sanderson, Julie N-acetylhistidine, a novel osmolyte in the lens of Atlantic salmon (Salmo salar L.) |
author_facet |
Rhodes, Jeremy D. Breck, Olav Waagbø, Rune Bjerkas, Ellen Sanderson, Julie |
author_sort |
Rhodes, Jeremy D. |
title |
N-acetylhistidine, a novel osmolyte in the lens of Atlantic salmon (Salmo salar L.) |
title_short |
N-acetylhistidine, a novel osmolyte in the lens of Atlantic salmon (Salmo salar L.) |
title_full |
N-acetylhistidine, a novel osmolyte in the lens of Atlantic salmon (Salmo salar L.) |
title_fullStr |
N-acetylhistidine, a novel osmolyte in the lens of Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
N-acetylhistidine, a novel osmolyte in the lens of Atlantic salmon (Salmo salar L.) |
title_sort |
n-acetylhistidine, a novel osmolyte in the lens of atlantic salmon (salmo salar l.) |
publishDate |
2010 |
url |
https://ueaeprints.uea.ac.uk/id/eprint/28409/ https://ueaeprints.uea.ac.uk/id/eprint/28409/1/Rhodes_et_al,_2010.pdf https://doi.org/10.1152/ajpregu.00214.2010 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://ueaeprints.uea.ac.uk/id/eprint/28409/1/Rhodes_et_al,_2010.pdf Rhodes, Jeremy D., Breck, Olav, Waagbø, Rune, Bjerkas, Ellen and Sanderson, Julie (2010) N-acetylhistidine, a novel osmolyte in the lens of Atlantic salmon (Salmo salar L.). AJP - Regulatory, Integrative and Comparative Physiology, 299 (4). R1075-R1081. doi:10.1152/ajpregu.00214.2010 |
op_doi |
https://doi.org/10.1152/ajpregu.00214.2010 |
container_title |
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
container_volume |
299 |
container_issue |
4 |
container_start_page |
R1075 |
op_container_end_page |
R1081 |
_version_ |
1766362377680322560 |