Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis
To improve the understanding of the piscirickettsiosis pathogenesis, the in vivo apoptosis modulation of peritoneal macrophages and lymphocytes was studied in juvenile Salmo salar intraperitoneally injected with Piscirickettsia salmonis. Five fish were sampled at post-exposure days 1, 5, 8 (preclini...
Published in: | Journal of Fish Diseases |
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Online Access: | http://hdl.handle.net/11447/1900 https://doi.org/10.1111/jfd.12660 |
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ftunivdesarrollo:oai:repositorio.udd.cl:11447/1900 2023-05-15T15:32:14+02:00 Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis Diaz, Spthefania Rojas, M Galleguillos, Marco Maturana, C Smith, P Cifuentes, F Contreras, I 2017 8 application/pdf http://hdl.handle.net/11447/1900 https://doi.org/10.1111/jfd.12660 en_US eng John Wiley & Sons Díaz S, Rojas ME, Galleguillos M, Maturana C, Smith PI, Cifuentes F, Contreras I, Smith PA. Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis. J Fish Dis. 2017 Dec;40(12):1895-1902 http://hdl.handle.net/11447/1900 http://dx.doi.org/10.1111/jfd.12660 apoptosis fish leukocytes Piscirickettsia salmon Artículo 2017 ftunivdesarrollo https://doi.org/10.1111/jfd.12660 2023-02-10T11:44:18Z To improve the understanding of the piscirickettsiosis pathogenesis, the in vivo apoptosis modulation of peritoneal macrophages and lymphocytes was studied in juvenile Salmo salar intraperitoneally injected with Piscirickettsia salmonis. Five fish were sampled at post-exposure days 1, 5, 8 (preclinical), 20 (clinical) and 40 (post-clinical period of the disease), and the leucocytes of their coelomic washings were analysed by flow cytometry (using the JC-1 cationic dye), TUNEL and cytology to detect apoptotic cells. A selective and temporal pattern of apoptosis modulation by P. salmonis infection was observed. Apoptosis in lymphocytes was not affected, whereas it was inhibited in macrophages but only during the preclinical stage of the induced piscirickettsiosis. Hence, it is postulated that P. salmonis inhibits macrophage apoptosis at the beginning of the disease development to survive, multiply and probably be transported inside these phagocytes; once this process is complete, macrophage apoptosis is no longer inhibited, thus facilitating the exit of the bacteria from the infected cells for continuing their life cycle. Article in Journal/Newspaper Atlantic salmon Salmo salar Repositorio Universidad del Desarrollo (UDD) Journal of Fish Diseases 40 12 1895 1902 |
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Open Polar |
collection |
Repositorio Universidad del Desarrollo (UDD) |
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ftunivdesarrollo |
language |
English |
topic |
apoptosis fish leukocytes Piscirickettsia salmon |
spellingShingle |
apoptosis fish leukocytes Piscirickettsia salmon Diaz, Spthefania Rojas, M Galleguillos, Marco Maturana, C Smith, P Cifuentes, F Contreras, I Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
topic_facet |
apoptosis fish leukocytes Piscirickettsia salmon |
description |
To improve the understanding of the piscirickettsiosis pathogenesis, the in vivo apoptosis modulation of peritoneal macrophages and lymphocytes was studied in juvenile Salmo salar intraperitoneally injected with Piscirickettsia salmonis. Five fish were sampled at post-exposure days 1, 5, 8 (preclinical), 20 (clinical) and 40 (post-clinical period of the disease), and the leucocytes of their coelomic washings were analysed by flow cytometry (using the JC-1 cationic dye), TUNEL and cytology to detect apoptotic cells. A selective and temporal pattern of apoptosis modulation by P. salmonis infection was observed. Apoptosis in lymphocytes was not affected, whereas it was inhibited in macrophages but only during the preclinical stage of the induced piscirickettsiosis. Hence, it is postulated that P. salmonis inhibits macrophage apoptosis at the beginning of the disease development to survive, multiply and probably be transported inside these phagocytes; once this process is complete, macrophage apoptosis is no longer inhibited, thus facilitating the exit of the bacteria from the infected cells for continuing their life cycle. |
format |
Article in Journal/Newspaper |
author |
Diaz, Spthefania Rojas, M Galleguillos, Marco Maturana, C Smith, P Cifuentes, F Contreras, I |
author_facet |
Diaz, Spthefania Rojas, M Galleguillos, Marco Maturana, C Smith, P Cifuentes, F Contreras, I |
author_sort |
Diaz, Spthefania |
title |
Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
title_short |
Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
title_full |
Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
title_fullStr |
Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
title_full_unstemmed |
Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
title_sort |
apoptosis inhibition of atlantic salmon (salmo salar) peritoneal macrophages by piscirickettsia salmonis |
publisher |
John Wiley & Sons |
publishDate |
2017 |
url |
http://hdl.handle.net/11447/1900 https://doi.org/10.1111/jfd.12660 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Díaz S, Rojas ME, Galleguillos M, Maturana C, Smith PI, Cifuentes F, Contreras I, Smith PA. Apoptosis inhibition of Atlantic salmon (Salmo salar) peritoneal macrophages by Piscirickettsia salmonis. J Fish Dis. 2017 Dec;40(12):1895-1902 http://hdl.handle.net/11447/1900 http://dx.doi.org/10.1111/jfd.12660 |
op_doi |
https://doi.org/10.1111/jfd.12660 |
container_title |
Journal of Fish Diseases |
container_volume |
40 |
container_issue |
12 |
container_start_page |
1895 |
op_container_end_page |
1902 |
_version_ |
1766362734004273152 |