Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar)
Atlantic salmon (Salmo salar) fed a carbohydrate-rich diet exhibit suboptimal growth performance, along with other metabolic disturbances. It is well known that gut microbes play a pivotal role in influencing metabolism of the host, and these microbes can be modified by the diet. The main goal of th...
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KEAI PUBLISHING LTD
2022
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Online Access: | https://repositoriodigital.uct.cl/handle/10925/4632 https://doi.org/10.1016/j.aninu.2022.04.003 |
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ftunivctemuco:oai:repositoriodigital.uct.cl:10925/4632 2023-05-15T15:30:25+02:00 Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) Villasante, Alejandro Ramirez, Carolina Rodriguez, Hector Dantagnan, Patricio Hernandez, Adrian Figueroa, Elias Romero, Jaime 2022-08-01T18:52:49Z https://repositoriodigital.uct.cl/handle/10925/4632 https://doi.org/10.1016/j.aninu.2022.04.003 en eng KEAI PUBLISHING LTD ANIMAL NUTRITION,Vol.10,261-279,2022 https://repositoriodigital.uct.cl/handle/10925/4632 doi:10.1016/j.aninu.2022.04.003 ANIMAL NUTRITION Microbiota Microbiome Atlantic salmon Steatosis Lactic acid bacteria Probiotics Article 2022 ftunivctemuco https://doi.org/10.1016/j.aninu.2022.04.003 2022-08-06T23:52:26Z Atlantic salmon (Salmo salar) fed a carbohydrate-rich diet exhibit suboptimal growth performance, along with other metabolic disturbances. It is well known that gut microbes play a pivotal role in influencing metabolism of the host, and these microbes can be modified by the diet. The main goal of the present study was to determine the effect of feeding graded levels of digestible carbohydrates to Atlantic salmon on the distal intestine digesta microbiota at 3 sampling times (i.e., weeks 4, 8 and 12), during a 12-week trial. A low carbohydrate-to-high protein diet (LC/HP, 0% wheat starch), a medium carbohydrate-to medium protein diet (MC/MP, 15% wheat starch) or a high carbohydrate-to-low protein diet (HC/LP, 30% wheat starch) was fed to triplicate fish tanks (27 to 28 fish per tank). We performed an in-depth characterization of the distal intestine digesta microbiota. Further, growth parameters, liver histology and the expression of genes involved in hepatic neolipogenesis in fish were measured. Fish fed a HC/LP diet showed greater hepatosomatic and viscerosomatic indexes (P = 0.026 and P = 0.018, respectively), lower final weight (P = 0.005), weight gain (P = 0.003), feed efficiency (P = 0.033) and growth rate (P = 0.003) compared with fish fed the LC/HP diet. Further, feeding salmon a high digestible carbohydrate diet caused greater lipid vacuolization, steatosis index (P = 0.007) and expression of fatty acid synthase (fas) and delta-6 fatty acyl desaturase (d6fad) (P = 0.001 and P = 0.001, respectively) in the liver compared with fish fed the LC/HP diet. Although, the major impact of feeding a carbohydrate-rich diet to Atlantic salmon in beta diversity of distal intestine digesta microbiota was observed at week 4 (HC/LP vs MC/MP and HC/LP vs LC/HP; P = 0.007 and P = 0.008, respectively) and week 8 (HC/LP vs MC/MP; P = 0.04), no differences between experimental groups were detected after 12 weeks of feeding. Finally, at the end of the trial, there was a negative correlation between lactic acid bacteria (LAB) ... Article in Journal/Newspaper Atlantic salmon Salmo salar Repositorio Académico de la Universidad Católica de Temuco (UCT) Animal Nutrition 10 261 279 |
institution |
Open Polar |
collection |
Repositorio Académico de la Universidad Católica de Temuco (UCT) |
op_collection_id |
ftunivctemuco |
language |
English |
topic |
Microbiota Microbiome Atlantic salmon Steatosis Lactic acid bacteria Probiotics |
spellingShingle |
Microbiota Microbiome Atlantic salmon Steatosis Lactic acid bacteria Probiotics Villasante, Alejandro Ramirez, Carolina Rodriguez, Hector Dantagnan, Patricio Hernandez, Adrian Figueroa, Elias Romero, Jaime Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
topic_facet |
Microbiota Microbiome Atlantic salmon Steatosis Lactic acid bacteria Probiotics |
description |
Atlantic salmon (Salmo salar) fed a carbohydrate-rich diet exhibit suboptimal growth performance, along with other metabolic disturbances. It is well known that gut microbes play a pivotal role in influencing metabolism of the host, and these microbes can be modified by the diet. The main goal of the present study was to determine the effect of feeding graded levels of digestible carbohydrates to Atlantic salmon on the distal intestine digesta microbiota at 3 sampling times (i.e., weeks 4, 8 and 12), during a 12-week trial. A low carbohydrate-to-high protein diet (LC/HP, 0% wheat starch), a medium carbohydrate-to medium protein diet (MC/MP, 15% wheat starch) or a high carbohydrate-to-low protein diet (HC/LP, 30% wheat starch) was fed to triplicate fish tanks (27 to 28 fish per tank). We performed an in-depth characterization of the distal intestine digesta microbiota. Further, growth parameters, liver histology and the expression of genes involved in hepatic neolipogenesis in fish were measured. Fish fed a HC/LP diet showed greater hepatosomatic and viscerosomatic indexes (P = 0.026 and P = 0.018, respectively), lower final weight (P = 0.005), weight gain (P = 0.003), feed efficiency (P = 0.033) and growth rate (P = 0.003) compared with fish fed the LC/HP diet. Further, feeding salmon a high digestible carbohydrate diet caused greater lipid vacuolization, steatosis index (P = 0.007) and expression of fatty acid synthase (fas) and delta-6 fatty acyl desaturase (d6fad) (P = 0.001 and P = 0.001, respectively) in the liver compared with fish fed the LC/HP diet. Although, the major impact of feeding a carbohydrate-rich diet to Atlantic salmon in beta diversity of distal intestine digesta microbiota was observed at week 4 (HC/LP vs MC/MP and HC/LP vs LC/HP; P = 0.007 and P = 0.008, respectively) and week 8 (HC/LP vs MC/MP; P = 0.04), no differences between experimental groups were detected after 12 weeks of feeding. Finally, at the end of the trial, there was a negative correlation between lactic acid bacteria (LAB) ... |
format |
Article in Journal/Newspaper |
author |
Villasante, Alejandro Ramirez, Carolina Rodriguez, Hector Dantagnan, Patricio Hernandez, Adrian Figueroa, Elias Romero, Jaime |
author_facet |
Villasante, Alejandro Ramirez, Carolina Rodriguez, Hector Dantagnan, Patricio Hernandez, Adrian Figueroa, Elias Romero, Jaime |
author_sort |
Villasante, Alejandro |
title |
Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
title_short |
Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
title_full |
Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
title_fullStr |
Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
title_full_unstemmed |
Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
title_sort |
dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (salmo salar) |
publisher |
KEAI PUBLISHING LTD |
publishDate |
2022 |
url |
https://repositoriodigital.uct.cl/handle/10925/4632 https://doi.org/10.1016/j.aninu.2022.04.003 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ANIMAL NUTRITION |
op_relation |
ANIMAL NUTRITION,Vol.10,261-279,2022 https://repositoriodigital.uct.cl/handle/10925/4632 doi:10.1016/j.aninu.2022.04.003 |
op_doi |
https://doi.org/10.1016/j.aninu.2022.04.003 |
container_title |
Animal Nutrition |
container_volume |
10 |
container_start_page |
261 |
op_container_end_page |
279 |
_version_ |
1766360874143973376 |