Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility
Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from energ...
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ftunivctemuco:oai:repositoriodigital.uct.cl:10925/3489 2023-05-15T15:31:46+02:00 Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility Lee Estevez, Manuel Herrera, Lisandra Diaz, Rommy Beltran, Jorge Figueroa Villalobos, Elías Dumorne, Kelly Ulloa Rodriguez, Patricio Short, Stefania Risopatron, Jennie Valdebenito Isler, Iván Farias, Jorge 2021-04-30T16:47:01Z http://repositoriodigital.uct.cl/handle/10925/3489 https://doi.org/10.1016/j.anireprosci.2019.106133 en eng ELSEVIER ANIMAL REPRODUCTION SCIENCE,Vol.209,,2019 http://repositoriodigital.uct.cl/handle/10925/3489 doi:10.1016/j.anireprosci.2019.106133 ANIMAL REPRODUCTION SCIENCE Cryopreservation Salmo salar Motility Spermatozoa PKA AMPK Article 2021 ftunivctemuco https://doi.org/10.1016/j.anireprosci.2019.106133 2021-05-01T23:51:31Z Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from energetic and cell signalling approaches. In this study, cAMP-dependent protein kinase (PKA) and AMP-activated kinase (AMPK) activities were quantified in fresh and cryopreserved spermatozoa of Atlantic salmon (Salmo salar); and the relation with motility was analysed. Results indicate there was a decrease in membrane integrity and motility in post-thawed spermatozoa compared to fresh samples, however, there was about 30% of cells with intact plasma membrane but incapable of motility. The PKA and AMPK activities were less after cryopreservation, indicating that loss of motility may be related to alteration of these key enzymes. Furthermore, PKA and AMPK activities were positively correlated with each other and with motility; and inhibition decreased motility, indicating there is a functional relationship between PKA and AMPK. The PKA inhibition also decreased AMPK activity, but results from protein-protein docking analyses indicated AMPK activation directly by PKA is unlikely, thus an indirect mechanism may exist. There have been no previous reports of these kinase actions in fish spermatozoa, making these findings worthy of assessment when there are future studies being planned, and may serve as base knowledge for optimization of cryopreservation procedures and development of biotechnologies to improve reproduction efficiency in the aquaculture industry. Article in Journal/Newspaper Atlantic salmon Salmo salar Repositorio Académico de la Universidad Católica de Temuco (UCT) Animal Reproduction Science 209 106133 |
institution |
Open Polar |
collection |
Repositorio Académico de la Universidad Católica de Temuco (UCT) |
op_collection_id |
ftunivctemuco |
language |
English |
topic |
Cryopreservation Salmo salar Motility Spermatozoa PKA AMPK |
spellingShingle |
Cryopreservation Salmo salar Motility Spermatozoa PKA AMPK Lee Estevez, Manuel Herrera, Lisandra Diaz, Rommy Beltran, Jorge Figueroa Villalobos, Elías Dumorne, Kelly Ulloa Rodriguez, Patricio Short, Stefania Risopatron, Jennie Valdebenito Isler, Iván Farias, Jorge Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility |
topic_facet |
Cryopreservation Salmo salar Motility Spermatozoa PKA AMPK |
description |
Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from energetic and cell signalling approaches. In this study, cAMP-dependent protein kinase (PKA) and AMP-activated kinase (AMPK) activities were quantified in fresh and cryopreserved spermatozoa of Atlantic salmon (Salmo salar); and the relation with motility was analysed. Results indicate there was a decrease in membrane integrity and motility in post-thawed spermatozoa compared to fresh samples, however, there was about 30% of cells with intact plasma membrane but incapable of motility. The PKA and AMPK activities were less after cryopreservation, indicating that loss of motility may be related to alteration of these key enzymes. Furthermore, PKA and AMPK activities were positively correlated with each other and with motility; and inhibition decreased motility, indicating there is a functional relationship between PKA and AMPK. The PKA inhibition also decreased AMPK activity, but results from protein-protein docking analyses indicated AMPK activation directly by PKA is unlikely, thus an indirect mechanism may exist. There have been no previous reports of these kinase actions in fish spermatozoa, making these findings worthy of assessment when there are future studies being planned, and may serve as base knowledge for optimization of cryopreservation procedures and development of biotechnologies to improve reproduction efficiency in the aquaculture industry. |
format |
Article in Journal/Newspaper |
author |
Lee Estevez, Manuel Herrera, Lisandra Diaz, Rommy Beltran, Jorge Figueroa Villalobos, Elías Dumorne, Kelly Ulloa Rodriguez, Patricio Short, Stefania Risopatron, Jennie Valdebenito Isler, Iván Farias, Jorge |
author_facet |
Lee Estevez, Manuel Herrera, Lisandra Diaz, Rommy Beltran, Jorge Figueroa Villalobos, Elías Dumorne, Kelly Ulloa Rodriguez, Patricio Short, Stefania Risopatron, Jennie Valdebenito Isler, Iván Farias, Jorge |
author_sort |
Lee Estevez, Manuel |
title |
Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility |
title_short |
Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility |
title_full |
Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility |
title_fullStr |
Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility |
title_full_unstemmed |
Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility |
title_sort |
effects of cryopreservation on camp-dependent protein kinase and amp-activated protein kinase in atlantic salmon (salmo salar) spermatozoa: relation with post-thaw motility |
publisher |
ELSEVIER |
publishDate |
2021 |
url |
http://repositoriodigital.uct.cl/handle/10925/3489 https://doi.org/10.1016/j.anireprosci.2019.106133 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ANIMAL REPRODUCTION SCIENCE |
op_relation |
ANIMAL REPRODUCTION SCIENCE,Vol.209,,2019 http://repositoriodigital.uct.cl/handle/10925/3489 doi:10.1016/j.anireprosci.2019.106133 |
op_doi |
https://doi.org/10.1016/j.anireprosci.2019.106133 |
container_title |
Animal Reproduction Science |
container_volume |
209 |
container_start_page |
106133 |
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1766362293106376704 |