Short-term storage of salmonids semen in a sodium alginate-based extender
Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our stu...
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ftunivctemuco:oai:repositoriodigital.uct.cl:10925/3357 2023-05-15T18:09:55+02:00 Short-term storage of salmonids semen in a sodium alginate-based extender Merino, O. Figueroa Villalobos, Elías Cheuqueman, C. Valdebenito Isler, Iván Isachenko, V. Isachenko, E. Sanchez, R. Farias, J. Risopatron, J. 2021-04-30T16:43:35Z http://repositoriodigital.uct.cl/handle/10925/3357 https://doi.org/10.1111/and.12661 en eng WILEY ANDROLOGIA,Vol.49,,2017 http://repositoriodigital.uct.cl/handle/10925/3357 doi:10.1111/and.12661 ANDROLOGIA antioxidants fish semen storage sodium alginate sperm function Article 2021 ftunivctemuco https://doi.org/10.1111/and.12661 2021-05-01T23:51:26Z Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our study was to analyse the effect of a sodium alginate-based extender on sperm function in the short-term storage of salmonids semen. Samples of Salmo salar, Oncorhynchus kisutch, and Oncorhynchus mykiss were stored in Storfish((R)) (Ext-C) and Storfish((R)) supplemented with sodium alginate (Ext-A) during 10days at 4 degrees C. After storage, motility, viability, mitochondrial membrane potential (mit), superoxide anion (O-2(-)) level and DNA fragmentation (DNA Frag) were assessed. Ext-A had positive effect in preservation of sperm motility, viability, mit, O-2(-) level and DNA integrity in the three species analysed compared to control samples. In Ext-A, the spermatozoa of S.salar and O.mykiss showed significantly higher motility, viability and mit than O.kisutch. However, O.kisutch and O.mykiss had significantly lower O-2(-) level than S.salar, and DNA fragmentation in O.kisutch and S.salar was significantly lower than in samples of O.mykiss (p<0.05). Dilution of salmonids semen in a sodium alginate-based extender is effective for protecting sperm quality during 10days of short-term storage. Article in Journal/Newspaper Salmo salar Repositorio Académico de la Universidad Católica de Temuco (UCT) Andrologia 49 5 e12661 |
institution |
Open Polar |
collection |
Repositorio Académico de la Universidad Católica de Temuco (UCT) |
op_collection_id |
ftunivctemuco |
language |
English |
topic |
antioxidants fish semen storage sodium alginate sperm function |
spellingShingle |
antioxidants fish semen storage sodium alginate sperm function Merino, O. Figueroa Villalobos, Elías Cheuqueman, C. Valdebenito Isler, Iván Isachenko, V. Isachenko, E. Sanchez, R. Farias, J. Risopatron, J. Short-term storage of salmonids semen in a sodium alginate-based extender |
topic_facet |
antioxidants fish semen storage sodium alginate sperm function |
description |
Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our study was to analyse the effect of a sodium alginate-based extender on sperm function in the short-term storage of salmonids semen. Samples of Salmo salar, Oncorhynchus kisutch, and Oncorhynchus mykiss were stored in Storfish((R)) (Ext-C) and Storfish((R)) supplemented with sodium alginate (Ext-A) during 10days at 4 degrees C. After storage, motility, viability, mitochondrial membrane potential (mit), superoxide anion (O-2(-)) level and DNA fragmentation (DNA Frag) were assessed. Ext-A had positive effect in preservation of sperm motility, viability, mit, O-2(-) level and DNA integrity in the three species analysed compared to control samples. In Ext-A, the spermatozoa of S.salar and O.mykiss showed significantly higher motility, viability and mit than O.kisutch. However, O.kisutch and O.mykiss had significantly lower O-2(-) level than S.salar, and DNA fragmentation in O.kisutch and S.salar was significantly lower than in samples of O.mykiss (p<0.05). Dilution of salmonids semen in a sodium alginate-based extender is effective for protecting sperm quality during 10days of short-term storage. |
format |
Article in Journal/Newspaper |
author |
Merino, O. Figueroa Villalobos, Elías Cheuqueman, C. Valdebenito Isler, Iván Isachenko, V. Isachenko, E. Sanchez, R. Farias, J. Risopatron, J. |
author_facet |
Merino, O. Figueroa Villalobos, Elías Cheuqueman, C. Valdebenito Isler, Iván Isachenko, V. Isachenko, E. Sanchez, R. Farias, J. Risopatron, J. |
author_sort |
Merino, O. |
title |
Short-term storage of salmonids semen in a sodium alginate-based extender |
title_short |
Short-term storage of salmonids semen in a sodium alginate-based extender |
title_full |
Short-term storage of salmonids semen in a sodium alginate-based extender |
title_fullStr |
Short-term storage of salmonids semen in a sodium alginate-based extender |
title_full_unstemmed |
Short-term storage of salmonids semen in a sodium alginate-based extender |
title_sort |
short-term storage of salmonids semen in a sodium alginate-based extender |
publisher |
WILEY |
publishDate |
2021 |
url |
http://repositoriodigital.uct.cl/handle/10925/3357 https://doi.org/10.1111/and.12661 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
ANDROLOGIA |
op_relation |
ANDROLOGIA,Vol.49,,2017 http://repositoriodigital.uct.cl/handle/10925/3357 doi:10.1111/and.12661 |
op_doi |
https://doi.org/10.1111/and.12661 |
container_title |
Andrologia |
container_volume |
49 |
container_issue |
5 |
container_start_page |
e12661 |
_version_ |
1766182612745846784 |