Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function
The in vitro and in vivo effect of prolactin (PRL) on kidney macrophages from Atlantic salmon (Salmo salar) was investigated under the assumption that PRL stimulates immune innate response in mammals. Kidney macrophages were treated two ways: first, cultured in RPMI 1640 medium containing 10, 25, 50...
Published in: | Fish Physiology and Biochemistry |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SPRINGER
2021
|
Subjects: | |
Online Access: | http://repositoriodigital.uct.cl/handle/10925/3346 https://doi.org/10.1007/s10695-013-9777-7 |
id |
ftunivctemuco:oai:repositoriodigital.uct.cl:10925/3346 |
---|---|
record_format |
openpolar |
spelling |
ftunivctemuco:oai:repositoriodigital.uct.cl:10925/3346 2023-05-15T15:30:22+02:00 Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function Paredes, Marco González Alfaro, Katerina Figueroa, Jaime Montiel-Eulefi, Enrique 2021-04-30T16:43:34Z http://repositoriodigital.uct.cl/handle/10925/3346 https://doi.org/10.1007/s10695-013-9777-7 en eng SPRINGER FISH PHYSIOLOGY AND BIOCHEMISTRY,Vol.39,1215-1221,2013 http://repositoriodigital.uct.cl/handle/10925/3346 doi:10.1007/s10695-013-9777-7 FISH PHYSIOLOGY AND BIOCHEMISTRY Salmo salar Prolactin Phagocytic activity Superoxide anion Article 2021 ftunivctemuco https://doi.org/10.1007/s10695-013-9777-7 2021-05-01T23:51:21Z The in vitro and in vivo effect of prolactin (PRL) on kidney macrophages from Atlantic salmon (Salmo salar) was investigated under the assumption that PRL stimulates immune innate response in mammals. Kidney macrophages were treated two ways: first, cultured in RPMI 1640 medium containing 10, 25, 50 and 100 ng/mL of PRL and second, isolated from a fish with a PRL-injected dose of 100 ng/Kg. Reduced nitro blue tetrazolium (formazan) was used to produce intracellular superoxide anion. Phagocytic activity of PRL was determined in treated cells by optical microscopy observation of phagocytized Congo red-stained yeast. Kidney lysozyme activity was measured in PRL-injected fish. In vitro and in vivo macrophages treated with PRL presented an enhanced superoxide anion production, elevated phagocytic index and increased phagocytic activity. Treated fish showed higher levels of lysozyme activity in the head kidney compared to the control. These results indicate that PRL-stimulated innate immune response in Atlantic salmon and future studies will allow us to assess the possibility of using PRL as an immunostimulant in the Chilean salmon industry. Article in Journal/Newspaper Atlantic salmon Salmo salar Repositorio Académico de la Universidad Católica de Temuco (UCT) Fish Physiology and Biochemistry 39 5 1215 1221 |
institution |
Open Polar |
collection |
Repositorio Académico de la Universidad Católica de Temuco (UCT) |
op_collection_id |
ftunivctemuco |
language |
English |
topic |
Salmo salar Prolactin Phagocytic activity Superoxide anion |
spellingShingle |
Salmo salar Prolactin Phagocytic activity Superoxide anion Paredes, Marco González Alfaro, Katerina Figueroa, Jaime Montiel-Eulefi, Enrique Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function |
topic_facet |
Salmo salar Prolactin Phagocytic activity Superoxide anion |
description |
The in vitro and in vivo effect of prolactin (PRL) on kidney macrophages from Atlantic salmon (Salmo salar) was investigated under the assumption that PRL stimulates immune innate response in mammals. Kidney macrophages were treated two ways: first, cultured in RPMI 1640 medium containing 10, 25, 50 and 100 ng/mL of PRL and second, isolated from a fish with a PRL-injected dose of 100 ng/Kg. Reduced nitro blue tetrazolium (formazan) was used to produce intracellular superoxide anion. Phagocytic activity of PRL was determined in treated cells by optical microscopy observation of phagocytized Congo red-stained yeast. Kidney lysozyme activity was measured in PRL-injected fish. In vitro and in vivo macrophages treated with PRL presented an enhanced superoxide anion production, elevated phagocytic index and increased phagocytic activity. Treated fish showed higher levels of lysozyme activity in the head kidney compared to the control. These results indicate that PRL-stimulated innate immune response in Atlantic salmon and future studies will allow us to assess the possibility of using PRL as an immunostimulant in the Chilean salmon industry. |
format |
Article in Journal/Newspaper |
author |
Paredes, Marco González Alfaro, Katerina Figueroa, Jaime Montiel-Eulefi, Enrique |
author_facet |
Paredes, Marco González Alfaro, Katerina Figueroa, Jaime Montiel-Eulefi, Enrique |
author_sort |
Paredes, Marco |
title |
Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function |
title_short |
Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function |
title_full |
Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function |
title_fullStr |
Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function |
title_full_unstemmed |
Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function |
title_sort |
immunomodulatory effect of prolactin on atlantic salmon (salmo salar) macrophage function |
publisher |
SPRINGER |
publishDate |
2021 |
url |
http://repositoriodigital.uct.cl/handle/10925/3346 https://doi.org/10.1007/s10695-013-9777-7 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
FISH PHYSIOLOGY AND BIOCHEMISTRY |
op_relation |
FISH PHYSIOLOGY AND BIOCHEMISTRY,Vol.39,1215-1221,2013 http://repositoriodigital.uct.cl/handle/10925/3346 doi:10.1007/s10695-013-9777-7 |
op_doi |
https://doi.org/10.1007/s10695-013-9777-7 |
container_title |
Fish Physiology and Biochemistry |
container_volume |
39 |
container_issue |
5 |
container_start_page |
1215 |
op_container_end_page |
1221 |
_version_ |
1766360807235387392 |