Morphometric of blastomeres in Salmo salar
For Salmo salar, there is a lack of information on the morphology of the first blastomeres formed during embryonic development and which could be used as a diagnostic tool for the first stages of development. The purpose of this investigation, therefore, was to characterize morphometrically the firs...
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ftunivctemuco:oai:repositoriodigital.uct.cl:10925/2649 2023-05-15T18:09:45+02:00 Morphometric of blastomeres in Salmo salar Effer Roldan, Brian Sanchez, Ruben R. Ubilla Madrid, Andrea Figueroa Villalobos, Elías Valdebenito Isler, Iván 2021-04-30T16:28:44Z http://repositoriodigital.uct.cl/handle/10925/2649 https://doi.org/10.1017/S0967199412000718 en eng CAMBRIDGE UNIV PRESS ZYGOTE,Vol.22,470-475,2014 http://repositoriodigital.uct.cl/handle/10925/2649 doi:10.1017/S0967199412000718 ZYGOTE Blastomeres Embryos Morphometric Salmo salar Article 2021 ftunivctemuco https://doi.org/10.1017/S0967199412000718 2021-05-01T23:51:03Z For Salmo salar, there is a lack of information on the morphology of the first blastomeres formed during embryonic development and which could be used as a diagnostic tool for the first stages of development. The purpose of this investigation, therefore, was to characterize morphometrically the first blastomeres of S. salar. From a pool of eggs incubated at 7.5 degrees C, 100 microphotographs of blastodiscs were extracted and analyzed at different incubation periods: 12, 14, 16, 20 or 24 h. Blastodiscs were characterized morphologically after 16, 20 or 24 h incubation, and classified into symmetric or asymmetric groups according to their morphology. The ratio of length (L) versus width (W) of each blastomere was determined, to establish its symmetry. In addition, 20 microphotographs of blastodiscs of normal appearance were analysed morphologically (control blastodisc: CB) for comparison (20 or 24 h). Results show that the first cleavage ends after 16 h of development. Seven categories were established during blastomere characterization: 47% normal (G1); 27% with dispersed margins (G2); 10% unequal (G3); 9% 'pie-shaped' (G4); 3% amorphous (G5); 2% three equal blastomeres and one different one (G6); and 2% with eccentric cleavage (G7). Although the incidence of abnormal cleavage in S. salar is uncertain, there is a potential for some asymmetries to be corrected during embryogenesis to generate viable individuals. More studies are necessary to correlate these abnormal cleavage patterns with indicators of quality in the later stages of embryogenesis in this species, to establish a quality assessment tool for gametes and/or embryos in salmonid species. Article in Journal/Newspaper Salmo salar Repositorio Académico de la Universidad Católica de Temuco (UCT) Zygote 22 4 470 475 |
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Open Polar |
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Repositorio Académico de la Universidad Católica de Temuco (UCT) |
op_collection_id |
ftunivctemuco |
language |
English |
topic |
Blastomeres Embryos Morphometric Salmo salar |
spellingShingle |
Blastomeres Embryos Morphometric Salmo salar Effer Roldan, Brian Sanchez, Ruben R. Ubilla Madrid, Andrea Figueroa Villalobos, Elías Valdebenito Isler, Iván Morphometric of blastomeres in Salmo salar |
topic_facet |
Blastomeres Embryos Morphometric Salmo salar |
description |
For Salmo salar, there is a lack of information on the morphology of the first blastomeres formed during embryonic development and which could be used as a diagnostic tool for the first stages of development. The purpose of this investigation, therefore, was to characterize morphometrically the first blastomeres of S. salar. From a pool of eggs incubated at 7.5 degrees C, 100 microphotographs of blastodiscs were extracted and analyzed at different incubation periods: 12, 14, 16, 20 or 24 h. Blastodiscs were characterized morphologically after 16, 20 or 24 h incubation, and classified into symmetric or asymmetric groups according to their morphology. The ratio of length (L) versus width (W) of each blastomere was determined, to establish its symmetry. In addition, 20 microphotographs of blastodiscs of normal appearance were analysed morphologically (control blastodisc: CB) for comparison (20 or 24 h). Results show that the first cleavage ends after 16 h of development. Seven categories were established during blastomere characterization: 47% normal (G1); 27% with dispersed margins (G2); 10% unequal (G3); 9% 'pie-shaped' (G4); 3% amorphous (G5); 2% three equal blastomeres and one different one (G6); and 2% with eccentric cleavage (G7). Although the incidence of abnormal cleavage in S. salar is uncertain, there is a potential for some asymmetries to be corrected during embryogenesis to generate viable individuals. More studies are necessary to correlate these abnormal cleavage patterns with indicators of quality in the later stages of embryogenesis in this species, to establish a quality assessment tool for gametes and/or embryos in salmonid species. |
format |
Article in Journal/Newspaper |
author |
Effer Roldan, Brian Sanchez, Ruben R. Ubilla Madrid, Andrea Figueroa Villalobos, Elías Valdebenito Isler, Iván |
author_facet |
Effer Roldan, Brian Sanchez, Ruben R. Ubilla Madrid, Andrea Figueroa Villalobos, Elías Valdebenito Isler, Iván |
author_sort |
Effer Roldan, Brian |
title |
Morphometric of blastomeres in Salmo salar |
title_short |
Morphometric of blastomeres in Salmo salar |
title_full |
Morphometric of blastomeres in Salmo salar |
title_fullStr |
Morphometric of blastomeres in Salmo salar |
title_full_unstemmed |
Morphometric of blastomeres in Salmo salar |
title_sort |
morphometric of blastomeres in salmo salar |
publisher |
CAMBRIDGE UNIV PRESS |
publishDate |
2021 |
url |
http://repositoriodigital.uct.cl/handle/10925/2649 https://doi.org/10.1017/S0967199412000718 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
ZYGOTE |
op_relation |
ZYGOTE,Vol.22,470-475,2014 http://repositoriodigital.uct.cl/handle/10925/2649 doi:10.1017/S0967199412000718 |
op_doi |
https://doi.org/10.1017/S0967199412000718 |
container_title |
Zygote |
container_volume |
22 |
container_issue |
4 |
container_start_page |
470 |
op_container_end_page |
475 |
_version_ |
1766182416355950592 |