Characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of Codfish (Gadus morhua)
In Portugal, Atlantic codfish (Gadus morhua) is usually consumed after dry salting; this process is carried out by mixing deboned codfish with food–grade marine salt followed by stacking in a tank for 6 days. Along the salting process, codfish incorporates salt as well as it is partially dried by th...
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The Italian Association of Chemical Engineering
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ftunivcplisboa:oai:repositorio.ucp.pt:10400.14/4845 2023-05-15T16:19:01+02:00 Characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of Codfish (Gadus morhua) Ferraro, Vincenza Cruz, Isabel B. Jorge, Ruben Ferreira Malcata, F. Xavier Castro, Paula M. L. Pintado, Manuela E. 2011-07-29T09:22:25Z http://hdl.handle.net/10400.14/4845 https://doi.org/10.3303/CET1021139 eng eng The Italian Association of Chemical Engineering info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F20653%2F2004/PT FERRARO, Vincenza …[et al.] - Characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of Codfish (Gadus morhua). Chemical Engineering Transactions. ISSN 1974-9791. Vol. 21 (2010), p. 829-834 9788895608051 2283-9216 http://hdl.handle.net/10400.14/4845 doi:10.3303/CET1021139 WOS:000286978600139 openAccess conferenceObject 2011 ftunivcplisboa https://doi.org/10.3303/CET1021139 2021-11-17T01:02:50Z In Portugal, Atlantic codfish (Gadus morhua) is usually consumed after dry salting; this process is carried out by mixing deboned codfish with food–grade marine salt followed by stacking in a tank for 6 days. Along the salting process, codfish incorporates salt as well as it is partially dried by the release of water - up to 22 % (w/w). Currently, this wastewater is treated as a residue not being further valorized. However, the presence of a significant amount of valuable compounds in this rest, such as amino acids and proteins, may allow for valorization opportunities not yet explored. The present work focuses on the identification of the nitrogen-containing compounds present in such wastewater. Total nitrogen (WSN), trichloroacetic acid-soluble nitrogen (TCASN) and phosphotungstic acid-soluble nitrogen (PTASN) were evaluated by the micro-Kjeldahl method; Biuret method was used for total protein determinations and SDS-Page was performed for protein molecular weight screening. The results revealed an increase of WSN, TCASN and PTASN with time, with corresponding values of 3.17 g/L (WSN), 1.62 g/L (TCASN) and 1.16 g/L (PTASN) by the end of the process; the evolution of WSN versus the released water was approximately constant during the salting process, as well as the ratios of TCASN/WSN and PTASN/WSN with values of 51.25 and 36.55 % (w/w) at equilibrium, respectively. info:eu-repo/semantics/publishedVersion Conference Object Gadus morhua Universidade Católica Portuguesa: Veritati - Repositório Institucional |
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Universidade Católica Portuguesa: Veritati - Repositório Institucional |
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ftunivcplisboa |
language |
English |
description |
In Portugal, Atlantic codfish (Gadus morhua) is usually consumed after dry salting; this process is carried out by mixing deboned codfish with food–grade marine salt followed by stacking in a tank for 6 days. Along the salting process, codfish incorporates salt as well as it is partially dried by the release of water - up to 22 % (w/w). Currently, this wastewater is treated as a residue not being further valorized. However, the presence of a significant amount of valuable compounds in this rest, such as amino acids and proteins, may allow for valorization opportunities not yet explored. The present work focuses on the identification of the nitrogen-containing compounds present in such wastewater. Total nitrogen (WSN), trichloroacetic acid-soluble nitrogen (TCASN) and phosphotungstic acid-soluble nitrogen (PTASN) were evaluated by the micro-Kjeldahl method; Biuret method was used for total protein determinations and SDS-Page was performed for protein molecular weight screening. The results revealed an increase of WSN, TCASN and PTASN with time, with corresponding values of 3.17 g/L (WSN), 1.62 g/L (TCASN) and 1.16 g/L (PTASN) by the end of the process; the evolution of WSN versus the released water was approximately constant during the salting process, as well as the ratios of TCASN/WSN and PTASN/WSN with values of 51.25 and 36.55 % (w/w) at equilibrium, respectively. info:eu-repo/semantics/publishedVersion |
format |
Conference Object |
author |
Ferraro, Vincenza Cruz, Isabel B. Jorge, Ruben Ferreira Malcata, F. Xavier Castro, Paula M. L. Pintado, Manuela E. |
spellingShingle |
Ferraro, Vincenza Cruz, Isabel B. Jorge, Ruben Ferreira Malcata, F. Xavier Castro, Paula M. L. Pintado, Manuela E. Characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of Codfish (Gadus morhua) |
author_facet |
Ferraro, Vincenza Cruz, Isabel B. Jorge, Ruben Ferreira Malcata, F. Xavier Castro, Paula M. L. Pintado, Manuela E. |
author_sort |
Ferraro, Vincenza |
title |
Characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of Codfish (Gadus morhua) |
title_short |
Characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of Codfish (Gadus morhua) |
title_full |
Characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of Codfish (Gadus morhua) |
title_fullStr |
Characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of Codfish (Gadus morhua) |
title_full_unstemmed |
Characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of Codfish (Gadus morhua) |
title_sort |
characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of codfish (gadus morhua) |
publisher |
The Italian Association of Chemical Engineering |
publishDate |
2011 |
url |
http://hdl.handle.net/10400.14/4845 https://doi.org/10.3303/CET1021139 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_relation |
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F20653%2F2004/PT FERRARO, Vincenza …[et al.] - Characterisation of soluble nitrogen and muscle proteins in wastewater throughout the salting process of Codfish (Gadus morhua). Chemical Engineering Transactions. ISSN 1974-9791. Vol. 21 (2010), p. 829-834 9788895608051 2283-9216 http://hdl.handle.net/10400.14/4845 doi:10.3303/CET1021139 WOS:000286978600139 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.3303/CET1021139 |
_version_ |
1766005333040300032 |