Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials
The synthesis of two biocatalysts based on a commercial Candida antarctica lipase B, CALB enzyme (E), physically immobilized on two silica supports, was carried out. The first support was a periodic mesoporous organosilica (PMO) and the second one was a commercial silica modified with octyl groups (...
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ftunivcordoba:oai:helvia.uco.es:10396/22060 2023-05-15T13:57:58+02:00 Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials Luna, Carlos Gascón-Pérez, Victoria López Tenllado, Francisco Javier Bautista, Felipa M. Verdugo-Escamilla, Cristóbal Aguado-Deblas, Laura Calero, Juan Romero, Antonio A. Luna, Diego Estévez, R. 2021 application/pdf http://hdl.handle.net/10396/22060 eng eng MDPI https://doi.org/10.3390/catal11111350 Gobierno de España. PID2019-104953RB-100 Junta de Andalucía. 1264113-R (CATOLIVAL) Junta de Andalucía. P18-RT-4822 http://hdl.handle.net/10396/22060 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess CC-BY Catalysts 11(11), 1350 (2021) Biofuel Ecodiesel Biodiesel Commercial CALB lipase Ordered mesoporous materials (PMO) Amorphous siliceous material MS3030 info:eu-repo/semantics/article 2021 ftunivcordoba https://doi.org/10.3390/catal11111350 2022-05-10T23:22:42Z The synthesis of two biocatalysts based on a commercial Candida antarctica lipase B, CALB enzyme (E), physically immobilized on two silica supports, was carried out. The first support was a periodic mesoporous organosilica (PMO) and the second one was a commercial silica modified with octyl groups (octyl-MS3030). The maximum enzyme load was 122 mg enzyme/g support on PMO and 288 mg enzyme/g support on octyl-MS3030. In addition, the biocatalytic efficiency was corroborated by two reaction tests based on the hydrolysis of p-nitrophenylacetate (p-NPA) and tributyrin (TB). The transesterification of sunflower oil with ethanol was carried out over the biocatalysts synthesized at the following reaction conditions: 6 mL sunflower oil, 1.75 mL EtOH, 30 °C, 25 μL NaOH 10 N and 300 rpm, attaining conversion values over 80% after 3 h of reaction time. According to the results obtained, we can confirm that these biocatalytic systems are viable candidates to develop, optimize and improve a new methodology to achieve the integration of glycerol in different monoacylglycerol molecules together with fatty acid ethyl esters (FAEE) molecules to obtain Ecodiesel. Article in Journal/Newspaper Antarc* Antarctica Helvia - Repositorio Institucional de la Universidad de Córdoba Catalysts 11 11 1350 |
institution |
Open Polar |
collection |
Helvia - Repositorio Institucional de la Universidad de Córdoba |
op_collection_id |
ftunivcordoba |
language |
English |
topic |
Biofuel Ecodiesel Biodiesel Commercial CALB lipase Ordered mesoporous materials (PMO) Amorphous siliceous material MS3030 |
spellingShingle |
Biofuel Ecodiesel Biodiesel Commercial CALB lipase Ordered mesoporous materials (PMO) Amorphous siliceous material MS3030 Luna, Carlos Gascón-Pérez, Victoria López Tenllado, Francisco Javier Bautista, Felipa M. Verdugo-Escamilla, Cristóbal Aguado-Deblas, Laura Calero, Juan Romero, Antonio A. Luna, Diego Estévez, R. Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
topic_facet |
Biofuel Ecodiesel Biodiesel Commercial CALB lipase Ordered mesoporous materials (PMO) Amorphous siliceous material MS3030 |
description |
The synthesis of two biocatalysts based on a commercial Candida antarctica lipase B, CALB enzyme (E), physically immobilized on two silica supports, was carried out. The first support was a periodic mesoporous organosilica (PMO) and the second one was a commercial silica modified with octyl groups (octyl-MS3030). The maximum enzyme load was 122 mg enzyme/g support on PMO and 288 mg enzyme/g support on octyl-MS3030. In addition, the biocatalytic efficiency was corroborated by two reaction tests based on the hydrolysis of p-nitrophenylacetate (p-NPA) and tributyrin (TB). The transesterification of sunflower oil with ethanol was carried out over the biocatalysts synthesized at the following reaction conditions: 6 mL sunflower oil, 1.75 mL EtOH, 30 °C, 25 μL NaOH 10 N and 300 rpm, attaining conversion values over 80% after 3 h of reaction time. According to the results obtained, we can confirm that these biocatalytic systems are viable candidates to develop, optimize and improve a new methodology to achieve the integration of glycerol in different monoacylglycerol molecules together with fatty acid ethyl esters (FAEE) molecules to obtain Ecodiesel. |
format |
Article in Journal/Newspaper |
author |
Luna, Carlos Gascón-Pérez, Victoria López Tenllado, Francisco Javier Bautista, Felipa M. Verdugo-Escamilla, Cristóbal Aguado-Deblas, Laura Calero, Juan Romero, Antonio A. Luna, Diego Estévez, R. |
author_facet |
Luna, Carlos Gascón-Pérez, Victoria López Tenllado, Francisco Javier Bautista, Felipa M. Verdugo-Escamilla, Cristóbal Aguado-Deblas, Laura Calero, Juan Romero, Antonio A. Luna, Diego Estévez, R. |
author_sort |
Luna, Carlos |
title |
Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
title_short |
Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
title_full |
Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
title_fullStr |
Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
title_full_unstemmed |
Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
title_sort |
enzymatic production of ecodiesel by using a commercial lipase calb, immobilized by physical adsorption on mesoporous organosilica materials |
publisher |
MDPI |
publishDate |
2021 |
url |
http://hdl.handle.net/10396/22060 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Catalysts 11(11), 1350 (2021) |
op_relation |
https://doi.org/10.3390/catal11111350 Gobierno de España. PID2019-104953RB-100 Junta de Andalucía. 1264113-R (CATOLIVAL) Junta de Andalucía. P18-RT-4822 http://hdl.handle.net/10396/22060 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/catal11111350 |
container_title |
Catalysts |
container_volume |
11 |
container_issue |
11 |
container_start_page |
1350 |
_version_ |
1766265920832929792 |