Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase
Background: Novozym 435, a commercial lipase from Candida antarctica, recombinant, expressed in Aspergillus niger, immobilized on macroporous acrylic resin, has been already described in the obtention of biodiesel. It is here evaluated in the production of a new biofuel that integrates the glycerol...
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ftunivcordoba:oai:helvia.uco.es:10396/12981 2023-05-15T13:57:58+02:00 Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase Luna, Carlos Verdugo-Escamilla, Cristóbal Sancho, Enrique D. Luna, Diego Calero, Juan Posadillo, Alejandro Bautista, Felipa M. Romero, Antonio A. 2014 application/pdf http://hdl.handle.net/10396/12981 eng eng SpringerOpen http://hdl.handle.net/10396/12981 https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess CC-BY-NC-ND Bioresources and Bioprocessing, 1:11 (2014) Biodiesel Enzyme biocatalysis Candida antarctica lipase B (CALB) N435 Response surface methodology info:eu-repo/semantics/article 2014 ftunivcordoba 2022-05-24T23:22:09Z Background: Novozym 435, a commercial lipase from Candida antarctica, recombinant, expressed in Aspergillus niger, immobilized on macroporous acrylic resin, has been already described in the obtention of biodiesel. It is here evaluated in the production of a new biofuel that integrates the glycerol as monoglyceride (MG) together with two fatty acid ethyl esters (FAEE) molecules by the application of 1,3-selective lipases in the ethanolysis reaction of sunflower oil. Results: Response surface methodology (RSM) is employed to estimate the effects of main reaction. Optimum conditions for the viscosity, selectivity, and conversion were determined using a multifactorial design of experiments with three factors run by the software Stat Graphics version XV.I. The selected experimental parameters were reaction temperature, oil/ethanol ratio and alkaline environment. On the basis of RSM analysis, the optimum conditions for synthesis were 1/6 oil/EtOH molar ratio, 30°C, and 12.5 μl of NaOH 10 N aqueous solutions, higher stirring than 300 rpm, for 2 h and 0.5 g of biocatalyst. Conclusions: These obtained results have proven a very good efficiency of the biocatalyst in the studied selective process. Furthermore, it was allowed sixteen times the successive reuse of the biocatalyst with good performance Article in Journal/Newspaper Antarc* Antarctica Helvia - Repositorio Institucional de la Universidad de Córdoba |
institution |
Open Polar |
collection |
Helvia - Repositorio Institucional de la Universidad de Córdoba |
op_collection_id |
ftunivcordoba |
language |
English |
topic |
Biodiesel Enzyme biocatalysis Candida antarctica lipase B (CALB) N435 Response surface methodology |
spellingShingle |
Biodiesel Enzyme biocatalysis Candida antarctica lipase B (CALB) N435 Response surface methodology Luna, Carlos Verdugo-Escamilla, Cristóbal Sancho, Enrique D. Luna, Diego Calero, Juan Posadillo, Alejandro Bautista, Felipa M. Romero, Antonio A. Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase |
topic_facet |
Biodiesel Enzyme biocatalysis Candida antarctica lipase B (CALB) N435 Response surface methodology |
description |
Background: Novozym 435, a commercial lipase from Candida antarctica, recombinant, expressed in Aspergillus niger, immobilized on macroporous acrylic resin, has been already described in the obtention of biodiesel. It is here evaluated in the production of a new biofuel that integrates the glycerol as monoglyceride (MG) together with two fatty acid ethyl esters (FAEE) molecules by the application of 1,3-selective lipases in the ethanolysis reaction of sunflower oil. Results: Response surface methodology (RSM) is employed to estimate the effects of main reaction. Optimum conditions for the viscosity, selectivity, and conversion were determined using a multifactorial design of experiments with three factors run by the software Stat Graphics version XV.I. The selected experimental parameters were reaction temperature, oil/ethanol ratio and alkaline environment. On the basis of RSM analysis, the optimum conditions for synthesis were 1/6 oil/EtOH molar ratio, 30°C, and 12.5 μl of NaOH 10 N aqueous solutions, higher stirring than 300 rpm, for 2 h and 0.5 g of biocatalyst. Conclusions: These obtained results have proven a very good efficiency of the biocatalyst in the studied selective process. Furthermore, it was allowed sixteen times the successive reuse of the biocatalyst with good performance |
format |
Article in Journal/Newspaper |
author |
Luna, Carlos Verdugo-Escamilla, Cristóbal Sancho, Enrique D. Luna, Diego Calero, Juan Posadillo, Alejandro Bautista, Felipa M. Romero, Antonio A. |
author_facet |
Luna, Carlos Verdugo-Escamilla, Cristóbal Sancho, Enrique D. Luna, Diego Calero, Juan Posadillo, Alejandro Bautista, Felipa M. Romero, Antonio A. |
author_sort |
Luna, Carlos |
title |
Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase |
title_short |
Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase |
title_full |
Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase |
title_fullStr |
Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase |
title_full_unstemmed |
Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase |
title_sort |
production of a biodiesel-like biofuel without glycerol generation, by using novozym 435, an immobilized candida antarctica lipase |
publisher |
SpringerOpen |
publishDate |
2014 |
url |
http://hdl.handle.net/10396/12981 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Bioresources and Bioprocessing, 1:11 (2014) |
op_relation |
http://hdl.handle.net/10396/12981 |
op_rights |
https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766265921001750528 |