The Combined Influence of Diving Physiology and Stressors on Immune Cell Function in a Deep Diving Monodontid and Three Shallow Diving Phocid Species
Marine mammals possess adaptations for repetitive and extended diving to great depths without suffering the ill effects seen in humans [e.g. decompression sickness (DCS)] which involve altered immune activity. In recent decades, DCS-like symptoms in marine mammals have increased concerns about marin...
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ftunivconn:oai:opencommons.uconn.edu:dissertations-6621 2023-05-15T15:41:49+02:00 The Combined Influence of Diving Physiology and Stressors on Immune Cell Function in a Deep Diving Monodontid and Three Shallow Diving Phocid Species Thompson, Laura A 2014-05-08T07:00:00Z application/pdf https://opencommons.uconn.edu/dissertations/405 https://opencommons.uconn.edu/cgi/viewcontent.cgi?article=6621&context=dissertations unknown OpenCommons@UConn https://opencommons.uconn.edu/dissertations/405 https://opencommons.uconn.edu/cgi/viewcontent.cgi?article=6621&context=dissertations Doctoral Dissertations Dive Pressure Marine Mammal Whale Beluga Seal Decompression Sickness Immune Function text 2014 ftunivconn 2022-07-11T18:49:24Z Marine mammals possess adaptations for repetitive and extended diving to great depths without suffering the ill effects seen in humans [e.g. decompression sickness (DCS)] which involve altered immune activity. In recent decades, DCS-like symptoms in marine mammals have increased concerns about marine mammal health and whether anthropogenic activities can interfere with adaptive dive responses, increasing susceptibility to dive related pathologies. The purpose of this work was to address these concerns by: 1) evaluating the in vitro response of marine mammal immune cells to increased pressure, 2) comparing the response of cells between baseline and stressor conditions, and 3) developing a non –invasive means of monitoring cortisol in belugas (Delphinapterus leucas). Blood samples were obtained from belugas during baseline and stressor (e.g. out of water examination, wild chase and capture) conditions, as well as from stranded harbor seals (Phoca vitulina), harp seals (Phoca groenlandica) and grey seals (Halichoerus grypus) at the time of admit to rehabilitation and again pre-release. Catecholamines and cortisol were measured to demonstrate a physiological stress response. Phagocytosis, lymphocyte proliferation and cell activation were compared between pressure exposed and non-exposed cells for each condition, between different pressure profiles and between conditions using mixed generalized linear models (α=0.05). The response of cells to pressure varied 1) between species, with baseline beluga samples and admit phocid samples showing opposite patterns of change than humans, 2) with stressor condition as responses differing from baseline but resembling human responses were detected for all stressor conditions in belugas, and 3) with exposure characteristics, with deeper exposures resulting in larger changes in phagocytosis but smaller changes in IL2R expression than shallower exposures. Blow (exhaled breath condensate) was also collected from belugas and validated as a matrix for monitoring cortisol using a ... Text Beluga Beluga* Delphinapterus leucas Phoca groenlandica Phoca vitulina University of Connecticut (UConn): DigitalCommons@UConn |
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University of Connecticut (UConn): DigitalCommons@UConn |
op_collection_id |
ftunivconn |
language |
unknown |
topic |
Dive Pressure Marine Mammal Whale Beluga Seal Decompression Sickness Immune Function |
spellingShingle |
Dive Pressure Marine Mammal Whale Beluga Seal Decompression Sickness Immune Function Thompson, Laura A The Combined Influence of Diving Physiology and Stressors on Immune Cell Function in a Deep Diving Monodontid and Three Shallow Diving Phocid Species |
topic_facet |
Dive Pressure Marine Mammal Whale Beluga Seal Decompression Sickness Immune Function |
description |
Marine mammals possess adaptations for repetitive and extended diving to great depths without suffering the ill effects seen in humans [e.g. decompression sickness (DCS)] which involve altered immune activity. In recent decades, DCS-like symptoms in marine mammals have increased concerns about marine mammal health and whether anthropogenic activities can interfere with adaptive dive responses, increasing susceptibility to dive related pathologies. The purpose of this work was to address these concerns by: 1) evaluating the in vitro response of marine mammal immune cells to increased pressure, 2) comparing the response of cells between baseline and stressor conditions, and 3) developing a non –invasive means of monitoring cortisol in belugas (Delphinapterus leucas). Blood samples were obtained from belugas during baseline and stressor (e.g. out of water examination, wild chase and capture) conditions, as well as from stranded harbor seals (Phoca vitulina), harp seals (Phoca groenlandica) and grey seals (Halichoerus grypus) at the time of admit to rehabilitation and again pre-release. Catecholamines and cortisol were measured to demonstrate a physiological stress response. Phagocytosis, lymphocyte proliferation and cell activation were compared between pressure exposed and non-exposed cells for each condition, between different pressure profiles and between conditions using mixed generalized linear models (α=0.05). The response of cells to pressure varied 1) between species, with baseline beluga samples and admit phocid samples showing opposite patterns of change than humans, 2) with stressor condition as responses differing from baseline but resembling human responses were detected for all stressor conditions in belugas, and 3) with exposure characteristics, with deeper exposures resulting in larger changes in phagocytosis but smaller changes in IL2R expression than shallower exposures. Blow (exhaled breath condensate) was also collected from belugas and validated as a matrix for monitoring cortisol using a ... |
format |
Text |
author |
Thompson, Laura A |
author_facet |
Thompson, Laura A |
author_sort |
Thompson, Laura A |
title |
The Combined Influence of Diving Physiology and Stressors on Immune Cell Function in a Deep Diving Monodontid and Three Shallow Diving Phocid Species |
title_short |
The Combined Influence of Diving Physiology and Stressors on Immune Cell Function in a Deep Diving Monodontid and Three Shallow Diving Phocid Species |
title_full |
The Combined Influence of Diving Physiology and Stressors on Immune Cell Function in a Deep Diving Monodontid and Three Shallow Diving Phocid Species |
title_fullStr |
The Combined Influence of Diving Physiology and Stressors on Immune Cell Function in a Deep Diving Monodontid and Three Shallow Diving Phocid Species |
title_full_unstemmed |
The Combined Influence of Diving Physiology and Stressors on Immune Cell Function in a Deep Diving Monodontid and Three Shallow Diving Phocid Species |
title_sort |
combined influence of diving physiology and stressors on immune cell function in a deep diving monodontid and three shallow diving phocid species |
publisher |
OpenCommons@UConn |
publishDate |
2014 |
url |
https://opencommons.uconn.edu/dissertations/405 https://opencommons.uconn.edu/cgi/viewcontent.cgi?article=6621&context=dissertations |
genre |
Beluga Beluga* Delphinapterus leucas Phoca groenlandica Phoca vitulina |
genre_facet |
Beluga Beluga* Delphinapterus leucas Phoca groenlandica Phoca vitulina |
op_source |
Doctoral Dissertations |
op_relation |
https://opencommons.uconn.edu/dissertations/405 https://opencommons.uconn.edu/cgi/viewcontent.cgi?article=6621&context=dissertations |
_version_ |
1766374706457346048 |