Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla
Anguillicoloides crassus is an invasive nematode parasite of the critically endangered European eel, Anguilla anguilla, and possibly one of the primary drivers of eel population collapse. The presence of the parasite has been shown to impact many features of eel physiology and life history. Early de...
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Online Access: | http://hdl.handle.net/10468/12468 https://doi.org/10.1017/S0031182021002146 |
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ftunivcollcork:oai:cora.ucc.ie:10468/12468 2023-08-27T04:03:59+02:00 Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla De Noia, M Poole, R. Kaufmann, Joshka Waters, C. Adams, C. McGinnity, Philip Llewellyn, M. 2022-01-19 application/pdf http://hdl.handle.net/10468/12468 https://doi.org/10.1017/S0031182021002146 en eng Cambridge University Press info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/15/IA/3028/IE/Wild farmed interactions in a changing world: formulation of a predictive methodology to inform environmental best practice to secure long-term sustainability of global wild and farm fish populations/ De Noia, M., Poole, R., Kaufmann, J., Waters, C., Adams, C., McGinnity, P. and Llewellyn, M. (2022) 'Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla', Parasitology. doi:10.1017/S0031182021002146 doi:10.1017/S0031182021002146 1469-8161 Parasitology http://hdl.handle.net/10468/12468 © 2022, the Authors. Published by Cambridge University Press. This material is free to view and download for personal use only. Not for re-distribution, re-sale or use in derivative works. Non-lethal test Fish parasite Genetic eDNA Article (peer-reviewed) 2022 ftunivcollcork https://doi.org/10.1017/S0031182021002146 2023-08-06T14:31:52Z Anguillicoloides crassus is an invasive nematode parasite of the critically endangered European eel, Anguilla anguilla, and possibly one of the primary drivers of eel population collapse. The presence of the parasite has been shown to impact many features of eel physiology and life history. Early detection of the parasite is vital to limit the spread of A. crassus, and to assess its potential impact on spawning biomass. However, until recently, accurate diagnosis of infection could only be achieved via necropsy. To support A. anguilla fisheries management in the context of A. crassus we developed a rapid, non-lethal, minimally invasive and in-situ DNA-based method to infer the presence of the parasite in the swim bladder. Screening of 131 wild eels was undertaken between 2017 and 2019 in Ireland and UK to validate the procedure. DNA extractions and PCR were conducted using both a Qiagen Stool kit at Glasgow University and in situ using Whatman qualitative filter paper No. 1 and a miniPCR DNA Discovery System™. Primers were specifically designed to target the cytochrome oxidase mtDNA gene region and in situ extraction and amplification takes approximately 3h for up to 16 individuals. Our in situ diagnostic procedure demonstrated Positive Predictive Values at 96% and Negative Predictive Values at 87% by comparison to necropsy data. Our method could be a valuable tool in the hands of fisheries managers to enable infection control and help protect this iconic but critically endangered species. Article in Journal/Newspaper Anguilla anguilla European eel University College Cork, Ireland: Cork Open Research Archive (CORA) Parasitology 1 7 |
institution |
Open Polar |
collection |
University College Cork, Ireland: Cork Open Research Archive (CORA) |
op_collection_id |
ftunivcollcork |
language |
English |
topic |
Non-lethal test Fish parasite Genetic eDNA |
spellingShingle |
Non-lethal test Fish parasite Genetic eDNA De Noia, M Poole, R. Kaufmann, Joshka Waters, C. Adams, C. McGinnity, Philip Llewellyn, M. Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla |
topic_facet |
Non-lethal test Fish parasite Genetic eDNA |
description |
Anguillicoloides crassus is an invasive nematode parasite of the critically endangered European eel, Anguilla anguilla, and possibly one of the primary drivers of eel population collapse. The presence of the parasite has been shown to impact many features of eel physiology and life history. Early detection of the parasite is vital to limit the spread of A. crassus, and to assess its potential impact on spawning biomass. However, until recently, accurate diagnosis of infection could only be achieved via necropsy. To support A. anguilla fisheries management in the context of A. crassus we developed a rapid, non-lethal, minimally invasive and in-situ DNA-based method to infer the presence of the parasite in the swim bladder. Screening of 131 wild eels was undertaken between 2017 and 2019 in Ireland and UK to validate the procedure. DNA extractions and PCR were conducted using both a Qiagen Stool kit at Glasgow University and in situ using Whatman qualitative filter paper No. 1 and a miniPCR DNA Discovery System™. Primers were specifically designed to target the cytochrome oxidase mtDNA gene region and in situ extraction and amplification takes approximately 3h for up to 16 individuals. Our in situ diagnostic procedure demonstrated Positive Predictive Values at 96% and Negative Predictive Values at 87% by comparison to necropsy data. Our method could be a valuable tool in the hands of fisheries managers to enable infection control and help protect this iconic but critically endangered species. |
format |
Article in Journal/Newspaper |
author |
De Noia, M Poole, R. Kaufmann, Joshka Waters, C. Adams, C. McGinnity, Philip Llewellyn, M. |
author_facet |
De Noia, M Poole, R. Kaufmann, Joshka Waters, C. Adams, C. McGinnity, Philip Llewellyn, M. |
author_sort |
De Noia, M |
title |
Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla |
title_short |
Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla |
title_full |
Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla |
title_fullStr |
Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla |
title_full_unstemmed |
Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla |
title_sort |
towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, anguillicoloides crassus, in european eel, anguilla anguilla |
publisher |
Cambridge University Press |
publishDate |
2022 |
url |
http://hdl.handle.net/10468/12468 https://doi.org/10.1017/S0031182021002146 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/15/IA/3028/IE/Wild farmed interactions in a changing world: formulation of a predictive methodology to inform environmental best practice to secure long-term sustainability of global wild and farm fish populations/ De Noia, M., Poole, R., Kaufmann, J., Waters, C., Adams, C., McGinnity, P. and Llewellyn, M. (2022) 'Towards an in-situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla', Parasitology. doi:10.1017/S0031182021002146 doi:10.1017/S0031182021002146 1469-8161 Parasitology http://hdl.handle.net/10468/12468 |
op_rights |
© 2022, the Authors. Published by Cambridge University Press. This material is free to view and download for personal use only. Not for re-distribution, re-sale or use in derivative works. |
op_doi |
https://doi.org/10.1017/S0031182021002146 |
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Parasitology |
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