Stabilization of immobilized lipases by treatment with metallic phosphate salts
Lipases from Thermomyces lanuginosus (TLL), Rhizomucor miehei (RML), Candida rugosa (CRL), forms A and B of lipase from Candida antarctica (CALA and CALB) and Eversa Transform 2.0 have been immobilized on octyl-agarose beads at two different loads (1 mg/g and saturated support) and treated with phos...
Published in: | International Journal of Biological Macromolecules |
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2022
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ftunivcmadrid:oai:www.ucm.es:75130 2023-05-15T13:45:45+02:00 Stabilization of immobilized lipases by treatment with metallic phosphate salts Guimarães, José R. Carballares, Diego Rocha Martin, Javier Tardioli, Paulo W. Fernandez-Lafuente, Roberto 2022-05-27 application/pdf https://eprints.ucm.es/id/eprint/75130/ https://eprints.ucm.es/id/eprint/75130/1/Guimar%C3%A4es,%20JR.%20et%20al.%202022.%20Stabilization%20of%20immobilized%20lipases%20by%20treatment.pdf https://doi.org/10.1016/j.ijbiomac.2022.05.167 en eng Elsevier https://eprints.ucm.es/id/eprint/75130/1/Guimar%C3%A4es,%20JR.%20et%20al.%202022.%20Stabilization%20of%20immobilized%20lipases%20by%20treatment.pdf info:eu-repo/semantics/restrictedAccess Bioquímica info:eu-repo/semantics/article PeerReviewed 2022 ftunivcmadrid https://doi.org/10.1016/j.ijbiomac.2022.05.167 2022-10-18T23:07:47Z Lipases from Thermomyces lanuginosus (TLL), Rhizomucor miehei (RML), Candida rugosa (CRL), forms A and B of lipase from Candida antarctica (CALA and CALB) and Eversa Transform 2.0 have been immobilized on octyl-agarose beads at two different loads (1 mg/g and saturated support) and treated with phosphate and/or some metallic salts (Zn2+, Co2+, Cu2+). They have been also immobilized on the support modified by the metallic phosphate, usually driving to biocatalyst with lower stability or marginal improvements. The effects of the phosphate/metal modification on enzyme features depended on the loading of the support. Some enzymes (TLL, CRL or CALA), mainly using the highly loaded biocatalysts, showed very significant improvement on enzyme stability after the treatment with some of the metal phosphates (next to a 20-fold factor), improvements that were not justified by the presence of metallic or phosphate ions in solution, as they had negative effects on enzyme stabilities. In some other cases, a significant increase in enzyme activity was detected (e.g., CALB). This could be explained by the modification of the nucleation places of the enzymes by the metallic phosphate, and this could help to explain the good results obtained in the nanoflower immobilization of many enzymes. Article in Journal/Newspaper Antarc* Antarctica Universidad Complutense de Madrid (UCM): E-Prints Complutense Rugosa ENVELOPE(-61.250,-61.250,-62.633,-62.633) International Journal of Biological Macromolecules 213 43 54 |
institution |
Open Polar |
collection |
Universidad Complutense de Madrid (UCM): E-Prints Complutense |
op_collection_id |
ftunivcmadrid |
language |
English |
topic |
Bioquímica |
spellingShingle |
Bioquímica Guimarães, José R. Carballares, Diego Rocha Martin, Javier Tardioli, Paulo W. Fernandez-Lafuente, Roberto Stabilization of immobilized lipases by treatment with metallic phosphate salts |
topic_facet |
Bioquímica |
description |
Lipases from Thermomyces lanuginosus (TLL), Rhizomucor miehei (RML), Candida rugosa (CRL), forms A and B of lipase from Candida antarctica (CALA and CALB) and Eversa Transform 2.0 have been immobilized on octyl-agarose beads at two different loads (1 mg/g and saturated support) and treated with phosphate and/or some metallic salts (Zn2+, Co2+, Cu2+). They have been also immobilized on the support modified by the metallic phosphate, usually driving to biocatalyst with lower stability or marginal improvements. The effects of the phosphate/metal modification on enzyme features depended on the loading of the support. Some enzymes (TLL, CRL or CALA), mainly using the highly loaded biocatalysts, showed very significant improvement on enzyme stability after the treatment with some of the metal phosphates (next to a 20-fold factor), improvements that were not justified by the presence of metallic or phosphate ions in solution, as they had negative effects on enzyme stabilities. In some other cases, a significant increase in enzyme activity was detected (e.g., CALB). This could be explained by the modification of the nucleation places of the enzymes by the metallic phosphate, and this could help to explain the good results obtained in the nanoflower immobilization of many enzymes. |
format |
Article in Journal/Newspaper |
author |
Guimarães, José R. Carballares, Diego Rocha Martin, Javier Tardioli, Paulo W. Fernandez-Lafuente, Roberto |
author_facet |
Guimarães, José R. Carballares, Diego Rocha Martin, Javier Tardioli, Paulo W. Fernandez-Lafuente, Roberto |
author_sort |
Guimarães, José R. |
title |
Stabilization of immobilized lipases by treatment with metallic phosphate salts |
title_short |
Stabilization of immobilized lipases by treatment with metallic phosphate salts |
title_full |
Stabilization of immobilized lipases by treatment with metallic phosphate salts |
title_fullStr |
Stabilization of immobilized lipases by treatment with metallic phosphate salts |
title_full_unstemmed |
Stabilization of immobilized lipases by treatment with metallic phosphate salts |
title_sort |
stabilization of immobilized lipases by treatment with metallic phosphate salts |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://eprints.ucm.es/id/eprint/75130/ https://eprints.ucm.es/id/eprint/75130/1/Guimar%C3%A4es,%20JR.%20et%20al.%202022.%20Stabilization%20of%20immobilized%20lipases%20by%20treatment.pdf https://doi.org/10.1016/j.ijbiomac.2022.05.167 |
long_lat |
ENVELOPE(-61.250,-61.250,-62.633,-62.633) |
geographic |
Rugosa |
geographic_facet |
Rugosa |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://eprints.ucm.es/id/eprint/75130/1/Guimar%C3%A4es,%20JR.%20et%20al.%202022.%20Stabilization%20of%20immobilized%20lipases%20by%20treatment.pdf |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.ijbiomac.2022.05.167 |
container_title |
International Journal of Biological Macromolecules |
container_volume |
213 |
container_start_page |
43 |
op_container_end_page |
54 |
_version_ |
1766230537614131200 |