Coimmobilization of lipases exhibiting three very different stability ranges. Reuse of the active enzymes and selective discarding of the inactivated ones

• Published in: International Journal of Biological Macromolecules
• Main Authors: Carballares, Diego, Rocha Martin, Javier, Fernandez Lafuente, Roberto
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2022
• Subjects: Bioquímica; Rugosa; Triton; Antarc*; Antarctica
• Online Access: https://eprints.ucm.es/id/eprint/73961/; https://eprints.ucm.es/id/eprint/73961/1/Carballares%20D.%20et%20al.%202022.%20Coimmobilization%20of%20lipases%20exhibiting%20three.pdf; https://doi.org/10.1016/j.ijbiomac.2022.02.084

Lipase B from Candida antarctica (CALB) and lipases from Candida rugosa (CRL) and Rhizomucor miehei (RML) have been coimmobilized on octyl and octyl-Asp agarose beads. CALB was much more stable than CRL, that was significantly more stable than RML. This forces the user to discard immobilized CALB an...