Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica)
Microorganisms living in extreme environments represent a huge reservoir of novel antimicrobial compounds and possibly of novel chemical families. Antarctica is one of the most extraordinary places on Earth and exhibits many distinctive features. Antarctic microorganisms are well known producers of...
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Online Access: | http://clok.uclan.ac.uk/20654/ http://clok.uclan.ac.uk/20654/1/20654%20marinedrugs-14-00083.pdf https://doi.org/10.3390/md14050083 |
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ftunivclancas:oai:clok.uclan.ac.uk:20654 2023-05-15T13:34:52+02:00 Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica) Tedesco, Pietro Maida, Isabel Palma Esposito, Fortunato Tortorella, Emiliana Subko, Karolina Ezeofor, Chidinma Zhang, Ying Tabudravu, Jioji Jaspars, Marcel Fani, Renato de Pascale, Donatella 2016-04-26 application/pdf http://clok.uclan.ac.uk/20654/ http://clok.uclan.ac.uk/20654/1/20654%20marinedrugs-14-00083.pdf https://doi.org/10.3390/md14050083 en eng MDPI http://clok.uclan.ac.uk/20654/1/20654%20marinedrugs-14-00083.pdf Tedesco, Pietro, Maida, Isabel, Palma Esposito, Fortunato, Tortorella, Emiliana, Subko, Karolina, Ezeofor, Chidinma, Zhang, Ying, Tabudravu, Jioji orcid:0000-0002-6930-6572 , Jaspars, Marcel et al (2016) Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica). Marine Drugs, 14 (5). p. 83. ISSN 1660-3397 doi:10.3390/md14050083 cc_by_4 CC-BY Bio-organic chemistry Article PeerReviewed 2016 ftunivclancas https://doi.org/10.3390/md14050083 2021-09-09T22:29:54Z Microorganisms living in extreme environments represent a huge reservoir of novel antimicrobial compounds and possibly of novel chemical families. Antarctica is one of the most extraordinary places on Earth and exhibits many distinctive features. Antarctic microorganisms are well known producers of valuable secondary metabolites. Specifically, several Antarctic strains have been reported to inhibit opportunistic human pathogens strains belonging to Burkholderia cepacia complex (Bcc). Herein, we applied a biodiscovery pipeline for the identification of anti-Bcc compounds. Antarctic sub-sea sediments were collected from the Ross Sea, and used to isolate 25 microorganisms, which were phylogenetically affiliated to three bacterial genera (Psychrobacter, Arthrobacter, and Pseudomonas) via sequencing and analysis of 16S rRNA genes. They were then subjected to a primary cell-based screening to determine their bioactivity against Bcc strains. Positive isolates were used to produce crude extracts from microbial spent culture media, to perform the secondary screening. Strain Pseudomonas BNT1 was then selected for bioassay-guided purification employing SPE and HPLC. Finally, LC-MS and NMR structurally resolved the purified bioactive compounds. With this strategy, we achieved the isolation of three rhamnolipids, two of which were new, endowed with high (MIC < 1 μg/mL) and unreported antimicrobial activity against Bcc strains. Article in Journal/Newspaper Antarc* Antarctic Antarctica Ross Sea University of Central Lancashire: CLOK - Central Lancashire Online Knowledge Antarctic Ross Sea Marine Drugs 14 5 83 |
institution |
Open Polar |
collection |
University of Central Lancashire: CLOK - Central Lancashire Online Knowledge |
op_collection_id |
ftunivclancas |
language |
English |
topic |
Bio-organic chemistry |
spellingShingle |
Bio-organic chemistry Tedesco, Pietro Maida, Isabel Palma Esposito, Fortunato Tortorella, Emiliana Subko, Karolina Ezeofor, Chidinma Zhang, Ying Tabudravu, Jioji Jaspars, Marcel Fani, Renato de Pascale, Donatella Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica) |
topic_facet |
Bio-organic chemistry |
description |
Microorganisms living in extreme environments represent a huge reservoir of novel antimicrobial compounds and possibly of novel chemical families. Antarctica is one of the most extraordinary places on Earth and exhibits many distinctive features. Antarctic microorganisms are well known producers of valuable secondary metabolites. Specifically, several Antarctic strains have been reported to inhibit opportunistic human pathogens strains belonging to Burkholderia cepacia complex (Bcc). Herein, we applied a biodiscovery pipeline for the identification of anti-Bcc compounds. Antarctic sub-sea sediments were collected from the Ross Sea, and used to isolate 25 microorganisms, which were phylogenetically affiliated to three bacterial genera (Psychrobacter, Arthrobacter, and Pseudomonas) via sequencing and analysis of 16S rRNA genes. They were then subjected to a primary cell-based screening to determine their bioactivity against Bcc strains. Positive isolates were used to produce crude extracts from microbial spent culture media, to perform the secondary screening. Strain Pseudomonas BNT1 was then selected for bioassay-guided purification employing SPE and HPLC. Finally, LC-MS and NMR structurally resolved the purified bioactive compounds. With this strategy, we achieved the isolation of three rhamnolipids, two of which were new, endowed with high (MIC < 1 μg/mL) and unreported antimicrobial activity against Bcc strains. |
format |
Article in Journal/Newspaper |
author |
Tedesco, Pietro Maida, Isabel Palma Esposito, Fortunato Tortorella, Emiliana Subko, Karolina Ezeofor, Chidinma Zhang, Ying Tabudravu, Jioji Jaspars, Marcel Fani, Renato de Pascale, Donatella |
author_facet |
Tedesco, Pietro Maida, Isabel Palma Esposito, Fortunato Tortorella, Emiliana Subko, Karolina Ezeofor, Chidinma Zhang, Ying Tabudravu, Jioji Jaspars, Marcel Fani, Renato de Pascale, Donatella |
author_sort |
Tedesco, Pietro |
title |
Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica) |
title_short |
Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica) |
title_full |
Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica) |
title_fullStr |
Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica) |
title_full_unstemmed |
Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica) |
title_sort |
antimicrobial activity of monoramnholipids produced by bacterial strains isolated from the ross sea (antarctica) |
publisher |
MDPI |
publishDate |
2016 |
url |
http://clok.uclan.ac.uk/20654/ http://clok.uclan.ac.uk/20654/1/20654%20marinedrugs-14-00083.pdf https://doi.org/10.3390/md14050083 |
geographic |
Antarctic Ross Sea |
geographic_facet |
Antarctic Ross Sea |
genre |
Antarc* Antarctic Antarctica Ross Sea |
genre_facet |
Antarc* Antarctic Antarctica Ross Sea |
op_relation |
http://clok.uclan.ac.uk/20654/1/20654%20marinedrugs-14-00083.pdf Tedesco, Pietro, Maida, Isabel, Palma Esposito, Fortunato, Tortorella, Emiliana, Subko, Karolina, Ezeofor, Chidinma, Zhang, Ying, Tabudravu, Jioji orcid:0000-0002-6930-6572 , Jaspars, Marcel et al (2016) Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica). Marine Drugs, 14 (5). p. 83. ISSN 1660-3397 doi:10.3390/md14050083 |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/md14050083 |
container_title |
Marine Drugs |
container_volume |
14 |
container_issue |
5 |
container_start_page |
83 |
_version_ |
1766058705098375168 |