Molecular mechanisms of reproduction in the Antarctic extremophile, Belgica antarctica
By Geoffrey Finch, Biology Advisor: Joshua Benoit Presentation ID: PM_ATRIUM17 Abstract: The Antarctic midge, Belgica antarctica, is a wingless, non-biting midge endemic to Antarctica. It is found in the Antarctic Peninsula and South Shetland Islands. The life cycle of this midge is unusually long d...
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ftunivcincinnati:oai:ojs.journals.uc.edu:article/1220 2023-05-15T13:51:36+02:00 Molecular mechanisms of reproduction in the Antarctic extremophile, Belgica antarctica Finch, Geoffrey Benoit, Joshua 2019-04-16 https://journals.uc.edu/index.php/Undergradshowcase/article/view/1220 unknown Cincinnati Library Publishing Services https://journals.uc.edu/index.php/Undergradshowcase/article/view/1220 Copyright (c) 2019 Undergraduate Scholarly Showcase Program (Spring 2019) Undergraduate Scholarly Showcase; 2019: Undergraduate Scholarly Showcase Program (Spring 2019) info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2019 ftunivcincinnati 2022-08-31T11:31:47Z By Geoffrey Finch, Biology Advisor: Joshua Benoit Presentation ID: PM_ATRIUM17 Abstract: The Antarctic midge, Belgica antarctica, is a wingless, non-biting midge endemic to Antarctica. It is found in the Antarctic Peninsula and South Shetland Islands. The life cycle of this midge is unusually long due to the short periods in which conditions for growth and development are favorable. The larval stage lasts for two years, and the adult stage is brief at about two weeks; adults mate in swarms, and females die shortly after oviposition. Eggs are laid suspended in a gel of unknown composition that is generated by the accessory gland. Many other aspects of reproduction in this and other midge species are not well understood. This project will characterize molecular mechanisms underlying reproduction in B. antarctica by examining differential gene expression in males, females, and larvae, as well as male and female accessory glands. In males, females, and larvae, 392, 1825, and 862 uniquely up-regulated genes were identified, respectively. For the accessory glands, 20 and 25 genes were enriched from the females and males, respectively. Gene ontology analyses were conducted to determine, where possible, the putative roles of genes differentially up-regulated between the sexes, or between whole-body and accessory gland tissues. Proteomic analyses were used to establish the composition of the egg-containing gel, and RNAseq data was used to determine the source of the proteins comprising the egg gel. Finally, a comparative analysis using genomes of several mosquito and midge species was conducted to gain insight into how conserved genes associated with reproduction are across lower flies (Nematocera). Article in Journal/Newspaper Antarc* Antarctic Antarctic midge Antarctic Peninsula Antarctica Belgica antarctica South Shetland Islands Journals@UC (University of Cincinnati) Antarctic Antarctic Peninsula Finch ENVELOPE(167.383,167.383,-72.567,-72.567) South Shetland Islands The Antarctic |
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Journals@UC (University of Cincinnati) |
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ftunivcincinnati |
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By Geoffrey Finch, Biology Advisor: Joshua Benoit Presentation ID: PM_ATRIUM17 Abstract: The Antarctic midge, Belgica antarctica, is a wingless, non-biting midge endemic to Antarctica. It is found in the Antarctic Peninsula and South Shetland Islands. The life cycle of this midge is unusually long due to the short periods in which conditions for growth and development are favorable. The larval stage lasts for two years, and the adult stage is brief at about two weeks; adults mate in swarms, and females die shortly after oviposition. Eggs are laid suspended in a gel of unknown composition that is generated by the accessory gland. Many other aspects of reproduction in this and other midge species are not well understood. This project will characterize molecular mechanisms underlying reproduction in B. antarctica by examining differential gene expression in males, females, and larvae, as well as male and female accessory glands. In males, females, and larvae, 392, 1825, and 862 uniquely up-regulated genes were identified, respectively. For the accessory glands, 20 and 25 genes were enriched from the females and males, respectively. Gene ontology analyses were conducted to determine, where possible, the putative roles of genes differentially up-regulated between the sexes, or between whole-body and accessory gland tissues. Proteomic analyses were used to establish the composition of the egg-containing gel, and RNAseq data was used to determine the source of the proteins comprising the egg gel. Finally, a comparative analysis using genomes of several mosquito and midge species was conducted to gain insight into how conserved genes associated with reproduction are across lower flies (Nematocera). |
format |
Article in Journal/Newspaper |
author |
Finch, Geoffrey Benoit, Joshua |
spellingShingle |
Finch, Geoffrey Benoit, Joshua Molecular mechanisms of reproduction in the Antarctic extremophile, Belgica antarctica |
author_facet |
Finch, Geoffrey Benoit, Joshua |
author_sort |
Finch, Geoffrey |
title |
Molecular mechanisms of reproduction in the Antarctic extremophile, Belgica antarctica |
title_short |
Molecular mechanisms of reproduction in the Antarctic extremophile, Belgica antarctica |
title_full |
Molecular mechanisms of reproduction in the Antarctic extremophile, Belgica antarctica |
title_fullStr |
Molecular mechanisms of reproduction in the Antarctic extremophile, Belgica antarctica |
title_full_unstemmed |
Molecular mechanisms of reproduction in the Antarctic extremophile, Belgica antarctica |
title_sort |
molecular mechanisms of reproduction in the antarctic extremophile, belgica antarctica |
publisher |
Cincinnati Library Publishing Services |
publishDate |
2019 |
url |
https://journals.uc.edu/index.php/Undergradshowcase/article/view/1220 |
long_lat |
ENVELOPE(167.383,167.383,-72.567,-72.567) |
geographic |
Antarctic Antarctic Peninsula Finch South Shetland Islands The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula Finch South Shetland Islands The Antarctic |
genre |
Antarc* Antarctic Antarctic midge Antarctic Peninsula Antarctica Belgica antarctica South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctic midge Antarctic Peninsula Antarctica Belgica antarctica South Shetland Islands |
op_source |
Undergraduate Scholarly Showcase; 2019: Undergraduate Scholarly Showcase Program (Spring 2019) |
op_relation |
https://journals.uc.edu/index.php/Undergradshowcase/article/view/1220 |
op_rights |
Copyright (c) 2019 Undergraduate Scholarly Showcase Program (Spring 2019) |
_version_ |
1766255567381200896 |