PCR-TTGE Analysis of 16S rRNA from Rainbow Trout (Oncorhynchus mykiss) Gut Microbiota Reveals Host- Specific Communities of Active Bacteria
Artículo de publicación ISI This study assessed the relative contributions of host genetics and diet in shaping the gut microbiota of rainbow trout. Full sibling fish from four unrelated families, each consisting of individuals derived from the mating of one male and one female belonging to a breedi...
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Online Access: | https://doi.org/10.1371/journal.pone.0031335 http://www.captura.uchile.cl/handle/2250/16732 |
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ftunivchilecap:oai:www.captura.uchile.cl:2250/16732 2023-05-15T15:32:23+02:00 PCR-TTGE Analysis of 16S rRNA from Rainbow Trout (Oncorhynchus mykiss) Gut Microbiota Reveals Host- Specific Communities of Active Bacteria Navarrete Wallace, Paola Alejandra Magne, Fabien Araneda, Cristián Fuentes, Pamela Barros, Luis Opazo, Rafael Espejo Torres, Romilio Hernán Romero Ormazábal, Jaime Moisés 2012-02-29 https://doi.org/10.1371/journal.pone.0031335 http://www.captura.uchile.cl/handle/2250/16732 en eng PUBLIC LIBRARY SCIENCE SALMO-SALAR L. ATLANTIC SALMON SOYBEAN-MEAL INTESTINAL MICROBIOTA PHYLOGENETIC ANALYSIS MOLECULAR ANALYSIS FISH-MEAL ZEBRAFISH WALBAUM DIVERSITY Artículo de Revista 2012 ftunivchilecap https://doi.org/10.1371/journal.pone.0031335 2013-12-20T10:29:15Z Artículo de publicación ISI This study assessed the relative contributions of host genetics and diet in shaping the gut microbiota of rainbow trout. Full sibling fish from four unrelated families, each consisting of individuals derived from the mating of one male and one female belonging to a breeding program, were fed diets containing either vegetable proteins or vegetable oils for two months in comparison to a control diet consisting of only fish protein and fish oil. Two parallel approaches were applied on the same samples: transcriptionally active bacterial populations were examined based on RNA analysis and were compared with bacterial populations obtained from DNA analysis. Comparison of temporal temperature gradient gel electrophoresis (TTGE) profiles from DNA and RNA showed important differences, indicating that active bacterial populations were better described by RNA analysis. Results showed that some bacterial groups were significantly (P<0.05) associated with specific families, indicating that microbiota composition may be influenced by the host. In addition, the effect of diet on microbiota composition was dependent on the trout family. Article in Journal/Newspaper Atlantic salmon Salmo salar Universidad de Chile: Captura Isi ENVELOPE(-38.550,-38.550,65.617,65.617) PLoS ONE 7 2 e31335 |
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Open Polar |
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Universidad de Chile: Captura |
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ftunivchilecap |
language |
English |
topic |
SALMO-SALAR L. ATLANTIC SALMON SOYBEAN-MEAL INTESTINAL MICROBIOTA PHYLOGENETIC ANALYSIS MOLECULAR ANALYSIS FISH-MEAL ZEBRAFISH WALBAUM DIVERSITY |
spellingShingle |
SALMO-SALAR L. ATLANTIC SALMON SOYBEAN-MEAL INTESTINAL MICROBIOTA PHYLOGENETIC ANALYSIS MOLECULAR ANALYSIS FISH-MEAL ZEBRAFISH WALBAUM DIVERSITY Navarrete Wallace, Paola Alejandra Magne, Fabien Araneda, Cristián Fuentes, Pamela Barros, Luis Opazo, Rafael Espejo Torres, Romilio Hernán Romero Ormazábal, Jaime Moisés PCR-TTGE Analysis of 16S rRNA from Rainbow Trout (Oncorhynchus mykiss) Gut Microbiota Reveals Host- Specific Communities of Active Bacteria |
topic_facet |
SALMO-SALAR L. ATLANTIC SALMON SOYBEAN-MEAL INTESTINAL MICROBIOTA PHYLOGENETIC ANALYSIS MOLECULAR ANALYSIS FISH-MEAL ZEBRAFISH WALBAUM DIVERSITY |
description |
Artículo de publicación ISI This study assessed the relative contributions of host genetics and diet in shaping the gut microbiota of rainbow trout. Full sibling fish from four unrelated families, each consisting of individuals derived from the mating of one male and one female belonging to a breeding program, were fed diets containing either vegetable proteins or vegetable oils for two months in comparison to a control diet consisting of only fish protein and fish oil. Two parallel approaches were applied on the same samples: transcriptionally active bacterial populations were examined based on RNA analysis and were compared with bacterial populations obtained from DNA analysis. Comparison of temporal temperature gradient gel electrophoresis (TTGE) profiles from DNA and RNA showed important differences, indicating that active bacterial populations were better described by RNA analysis. Results showed that some bacterial groups were significantly (P<0.05) associated with specific families, indicating that microbiota composition may be influenced by the host. In addition, the effect of diet on microbiota composition was dependent on the trout family. |
format |
Article in Journal/Newspaper |
author |
Navarrete Wallace, Paola Alejandra Magne, Fabien Araneda, Cristián Fuentes, Pamela Barros, Luis Opazo, Rafael Espejo Torres, Romilio Hernán Romero Ormazábal, Jaime Moisés |
author_facet |
Navarrete Wallace, Paola Alejandra Magne, Fabien Araneda, Cristián Fuentes, Pamela Barros, Luis Opazo, Rafael Espejo Torres, Romilio Hernán Romero Ormazábal, Jaime Moisés |
author_sort |
Navarrete Wallace, Paola Alejandra |
title |
PCR-TTGE Analysis of 16S rRNA from Rainbow Trout (Oncorhynchus mykiss) Gut Microbiota Reveals Host- Specific Communities of Active Bacteria |
title_short |
PCR-TTGE Analysis of 16S rRNA from Rainbow Trout (Oncorhynchus mykiss) Gut Microbiota Reveals Host- Specific Communities of Active Bacteria |
title_full |
PCR-TTGE Analysis of 16S rRNA from Rainbow Trout (Oncorhynchus mykiss) Gut Microbiota Reveals Host- Specific Communities of Active Bacteria |
title_fullStr |
PCR-TTGE Analysis of 16S rRNA from Rainbow Trout (Oncorhynchus mykiss) Gut Microbiota Reveals Host- Specific Communities of Active Bacteria |
title_full_unstemmed |
PCR-TTGE Analysis of 16S rRNA from Rainbow Trout (Oncorhynchus mykiss) Gut Microbiota Reveals Host- Specific Communities of Active Bacteria |
title_sort |
pcr-ttge analysis of 16s rrna from rainbow trout (oncorhynchus mykiss) gut microbiota reveals host- specific communities of active bacteria |
publisher |
PUBLIC LIBRARY SCIENCE |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pone.0031335 http://www.captura.uchile.cl/handle/2250/16732 |
long_lat |
ENVELOPE(-38.550,-38.550,65.617,65.617) |
geographic |
Isi |
geographic_facet |
Isi |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_doi |
https://doi.org/10.1371/journal.pone.0031335 |
container_title |
PLoS ONE |
container_volume |
7 |
container_issue |
2 |
container_start_page |
e31335 |
_version_ |
1766362892748193792 |