UHPLC-MS metabolomic fingerprinting, antioxidant, and enzyme inhibition activities of himantormia lugubris from Antarctica

Himantormia lugubris is a Chilean native small lichen shrub growing in the Antarctica region. In this study, the metabolite fingerprinting and the antioxidant and enzyme inhibitory potential from this species and its four major isolated compounds were investigated for the first time. Using ultra-hig...

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Bibliographic Details
Published in:Metabolites
Main Authors: Areche Medina, Carlos Alberto, Romero Parra, Javier Hernán, Sepúlveda, Beatriz, García Beltrán, Olimpo, Simirgiotis, Mario J.
Format: Article in Journal/Newspaper
Language:English
Published: MDPI 2022
Subjects:
Himantormia
Phenolics
Enzyme inhibition
Native lichens
Antioxidant
Depsides
Dibenzofurans
Antarctica
Alzheimer
Inach
Antarc*
antartic*
Instituto Antartico Chileno
Online Access:https://doi.org/10.3390/metabo12060560
https://repositorio.uchile.cl/handle/2250/186926
Description
Summary:Himantormia lugubris is a Chilean native small lichen shrub growing in the Antarctica region. In this study, the metabolite fingerprinting and the antioxidant and enzyme inhibitory potential from this species and its four major isolated compounds were investigated for the first time. Using ultra-high performance liquid chromatography coupled to quadrupole-Orbitrap mass spectrometry analysis (UHPLC-Q-Orbitrap-MS), several metabolites were identified including specific compounds as chemotaxonomical markers, while major metabolites were quantified in this species. A good inhibition activity against cholinesterase (acetylcholinesterase (AChE) IC50: 12.38 +/- 0.09 mu g/mL, butyrylcholinesterase (BChE) IC50: 31.54 +/- 0.20 mu g/mL) and tyrosinase (22.32 +/- 0.21 mu g/mL) enzymes of the alcoholic extract and the main compounds (IC50: 28.82 +/- 0.10 mu g/mL, 36.43 +/- 0.08 mu g/mL, and 7.25 +/- 0.18 mu g/mL, respectively, for the most active phenolic atranol) was found. The extract showed a total phenolic content of 47.4 + 0.0 mg of gallic acid equivalents/g. In addition, antioxidant activity was assessed using bleaching of DPPH and ORAC (IC50: 75.3 +/- 0.02 mu g/mL and 32.7 +/- 0.7 mu mol Trolox/g lichen, respectively) and FRAP (27.8 +/- 0.0 mu mol Trolox equivalent/g) experiments. The findings suggest that H. lugubris is a rich source of bioactive compounds with potentiality in the prevention of neurodegenerative or noncommunicable chronic diseases. Instituto Antartico Chileno INACh RT 16-17 Versión publicada - versión final del editor

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