Surface ammonia-oxidizer abundance during the late summer in the West Antarctic Coastal System
Marine ammonia oxidizers that oxidize ammonium to nitrite are abundant in polar waters, especially during the winter in the deeper mixed-layer of West Antarctic Peninsula (WAP) waters. However, the activity and abundance of ammonia-oxidizers during the summer in surface coastal Antarctic waters rema...
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ftunivchile:oai:repositorio.uchile.cl:2250/186351 2023-05-15T14:01:29+02:00 Surface ammonia-oxidizer abundance during the late summer in the West Antarctic Coastal System Alcamán Arias, María E. Cifuentes Anticevic, Jerónimo Diez, Beatriz Testa, Giovanni Troncoso, Macarena Bello, Estrella Farías, Laura 2022 application/pdf https://doi.org/10.3389/fmicb.2022.821902 https://repositorio.uchile.cl/handle/2250/186351 en eng Frontiers Media Frontiers in Microbiology March 2022 Volume 13 Article Number 821902 doi:10.3389/fmicb.2022.821902 https://repositorio.uchile.cl/handle/2250/186351 Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ CC-BY-NC-ND Frontiers in Microbiology Nitrification Ammonia-oxidizers Western Antarctic Peninsula Archaea Bacteria Photic layer Artículo de revista 2022 ftunivchile https://doi.org/10.3389/fmicb.2022.821902 2022-07-02T23:49:28Z Marine ammonia oxidizers that oxidize ammonium to nitrite are abundant in polar waters, especially during the winter in the deeper mixed-layer of West Antarctic Peninsula (WAP) waters. However, the activity and abundance of ammonia-oxidizers during the summer in surface coastal Antarctic waters remain unclear. In this study, the ammonia-oxidation rates, abundance and identity of ammonia-oxidizing bacteria (AOB) and archaea (AOA) were evaluated in the marine surface layer (to 30m depth) in Chile Bay (Greenwich Island, WAP) over three consecutive late-summer periods (2017, 2018, and 2019). Ammoniaoxidation rates of 68.31nmolNL−1 day−1 (2018) and 37.28nmolNL−1 day−1 (2019) were detected from illuminated 2m seawater incubations. However, high ammonia-oxidation rates between 267.75 and 109.38nmolNL−1 day−1 were obtained under the dark condition at 30m in 2018 and 2019, respectively. During the late-summer sampling periods both stratifying and mixing events occurring in the water column over short timescales (February–March). Metagenomic analysis of seven nitrogen cycle modules revealed the presence of ammonia-oxidizers, such as the Archaea Nitrosopumilus and the Bacteria Nitrosomonas and Nitrosospira, with AOA often being more abundant than AOB. However, quantification of specific amoA gene transcripts showed number of AOB being two orders of magnitude higher than AOA, with Nitrosomonas representing the most transcriptionally active AOB in the surface waters. Additionally, Candidatus Nitrosopelagicus and Nitrosopumilus, phylogenetically related to surface members of the NP-ε and NP-γ clades respectively, were the predominant AOA. Our findings expand the known distribution of ammonium-oxidizers to the marine surface layer, exposing their potential ecological role in supporting the marine Antarctic system during the productive summer periods. ANID/PFCHA/Doctorado Nacional 2017 21170561 Versión publicada - versión final del editor Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Greenwich Island Universidad de Chile: Repositorio académico Antarctic Antarctic Peninsula Greenwich Greenwich Island ENVELOPE(-59.783,-59.783,-62.517,-62.517) Frontiers in Microbiology 13 |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
Nitrification Ammonia-oxidizers Western Antarctic Peninsula Archaea Bacteria Photic layer |
spellingShingle |
Nitrification Ammonia-oxidizers Western Antarctic Peninsula Archaea Bacteria Photic layer Alcamán Arias, María E. Cifuentes Anticevic, Jerónimo Diez, Beatriz Testa, Giovanni Troncoso, Macarena Bello, Estrella Farías, Laura Surface ammonia-oxidizer abundance during the late summer in the West Antarctic Coastal System |
topic_facet |
Nitrification Ammonia-oxidizers Western Antarctic Peninsula Archaea Bacteria Photic layer |
description |
Marine ammonia oxidizers that oxidize ammonium to nitrite are abundant in polar waters, especially during the winter in the deeper mixed-layer of West Antarctic Peninsula (WAP) waters. However, the activity and abundance of ammonia-oxidizers during the summer in surface coastal Antarctic waters remain unclear. In this study, the ammonia-oxidation rates, abundance and identity of ammonia-oxidizing bacteria (AOB) and archaea (AOA) were evaluated in the marine surface layer (to 30m depth) in Chile Bay (Greenwich Island, WAP) over three consecutive late-summer periods (2017, 2018, and 2019). Ammoniaoxidation rates of 68.31nmolNL−1 day−1 (2018) and 37.28nmolNL−1 day−1 (2019) were detected from illuminated 2m seawater incubations. However, high ammonia-oxidation rates between 267.75 and 109.38nmolNL−1 day−1 were obtained under the dark condition at 30m in 2018 and 2019, respectively. During the late-summer sampling periods both stratifying and mixing events occurring in the water column over short timescales (February–March). Metagenomic analysis of seven nitrogen cycle modules revealed the presence of ammonia-oxidizers, such as the Archaea Nitrosopumilus and the Bacteria Nitrosomonas and Nitrosospira, with AOA often being more abundant than AOB. However, quantification of specific amoA gene transcripts showed number of AOB being two orders of magnitude higher than AOA, with Nitrosomonas representing the most transcriptionally active AOB in the surface waters. Additionally, Candidatus Nitrosopelagicus and Nitrosopumilus, phylogenetically related to surface members of the NP-ε and NP-γ clades respectively, were the predominant AOA. Our findings expand the known distribution of ammonium-oxidizers to the marine surface layer, exposing their potential ecological role in supporting the marine Antarctic system during the productive summer periods. ANID/PFCHA/Doctorado Nacional 2017 21170561 Versión publicada - versión final del editor |
format |
Article in Journal/Newspaper |
author |
Alcamán Arias, María E. Cifuentes Anticevic, Jerónimo Diez, Beatriz Testa, Giovanni Troncoso, Macarena Bello, Estrella Farías, Laura |
author_facet |
Alcamán Arias, María E. Cifuentes Anticevic, Jerónimo Diez, Beatriz Testa, Giovanni Troncoso, Macarena Bello, Estrella Farías, Laura |
author_sort |
Alcamán Arias, María E. |
title |
Surface ammonia-oxidizer abundance during the late summer in the West Antarctic Coastal System |
title_short |
Surface ammonia-oxidizer abundance during the late summer in the West Antarctic Coastal System |
title_full |
Surface ammonia-oxidizer abundance during the late summer in the West Antarctic Coastal System |
title_fullStr |
Surface ammonia-oxidizer abundance during the late summer in the West Antarctic Coastal System |
title_full_unstemmed |
Surface ammonia-oxidizer abundance during the late summer in the West Antarctic Coastal System |
title_sort |
surface ammonia-oxidizer abundance during the late summer in the west antarctic coastal system |
publisher |
Frontiers Media |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.821902 https://repositorio.uchile.cl/handle/2250/186351 |
long_lat |
ENVELOPE(-59.783,-59.783,-62.517,-62.517) |
geographic |
Antarctic Antarctic Peninsula Greenwich Greenwich Island |
geographic_facet |
Antarctic Antarctic Peninsula Greenwich Greenwich Island |
genre |
Antarc* Antarctic Antarctic Peninsula Greenwich Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Greenwich Island |
op_source |
Frontiers in Microbiology |
op_relation |
Frontiers in Microbiology March 2022 Volume 13 Article Number 821902 doi:10.3389/fmicb.2022.821902 https://repositorio.uchile.cl/handle/2250/186351 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.3389/fmicb.2022.821902 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1766271325513449472 |