Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) release Reactive Oxygen Species (ROS) as secondary Mn(II) oxidation mechanisms to avoid toxicity
Manganese (Mn)-oxidizing bacteria (MnOxb) are an essential group of microorganisms that oxidize soluble Mn(II) to form precipitate Mn(III) minerals, playing a crucial role in soil formation. The Fildes Peninsula is one of the fastest-warming areas globally and, therefore, the maritime Antarctic soil...
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ftunivchile:oai:repositorio.uchile.cl:2250/184036 2023-05-15T13:38:36+02:00 Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) release Reactive Oxygen Species (ROS) as secondary Mn(II) oxidation mechanisms to avoid toxicity Jofré, Ignacio Matus, Francisco Mendoza, Daniela Nájera De Ferrari, Francisco Merino, Carolina 2021 application/pdf https://doi.org/10.3390/biology10101004 https://repositorio.uchile.cl/handle/2250/184036 en eng MDPI Biology 2021, 10, 1004 doi:10.3390/biology10101004 https://repositorio.uchile.cl/handle/2250/184036 Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ CC-BY-NC-ND Biology ROS Mn oxidation Antarctic soil Artículo de revista 2021 ftunivchile https://doi.org/10.3390/biology10101004 2022-03-27T00:49:32Z Manganese (Mn)-oxidizing bacteria (MnOxb) are an essential group of microorganisms that oxidize soluble Mn(II) to form precipitate Mn(III) minerals, playing a crucial role in soil formation. The Fildes Peninsula is one of the fastest-warming areas globally and, therefore, the maritime Antarctic soils from this pivotal location allow for the examination of the effect of temperature on bacterial communities. The temperature causes an increase in the microbial respiratory rate, producing reactive oxygen species (ROS), which are harmful to bacteria. We evaluate an evasive secondary non-enzymatic mechanism for ROS production under increasing temperature in MnOxb isolated from Antarctic soils. Bacteria produce ROS capable of oxidizing Mn(II) as temperature increases, contributing to the enzymatic pathway protecting microbial cells from Mn(II) toxicity. In addition, we determine that certain strains, such as Arthobacter oxydans, can use these ROS as mechanisms to protect themselves from Mn toxicity at high concentrations. In conclusion, we describe a secondary mechanism of Mn(II) oxidation in bacterial strains of Antarctic soils. Manganese (Mn) oxidation is performed through oxidative Mn-oxidizing bacteria (MnOxb) as the main bio-weathering mechanism for Mn(III/IV) deposits during soil formation. However, with an increase in temperature, the respiration rate also increases, producing Reactive Oxygen Species (ROS) as by-products, which are harmful to microbial cells. We hypothesize that bacterial ROS oxidize Mn(II) to Mn(III/IV) as a secondary non-enzymatic temperature-dependent mechanism for cell protection. Fourteen MnOxb were isolated from Antarctic soils under the global warming effect, and peroxidase (PO) activity, ROS, and Mn(III/IV) production were evaluated for 120 h of incubation at 4 degrees C, 15 degrees C, and 30 degrees C. ROS contributions to Mn oxidation were evaluated in Arthrobacter oxydans under antioxidant (Trolox) and ROS-stimulated (menadione) conditions. The Mn(III/IV) concentration increased with temperature and positively correlated with ROS production. ROS scavenging with Trolox depleted the Mn oxidation, and ROS-stimulant increased the Mn precipitation in A. oxydans. Increasing the Mn(II) concentration caused a reduction in the membrane potential and bacterial viability, which resulted in Mn precipitation on the bacteria surface. In conclusion, bacterial ROS production serves as a complementary non-enzymatic temperature-dependent mechanism for Mn(II) oxidation as a response in warming environments. INACH RT_23_17 ANID Fondecyt N3200758 Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT N 1180521 Network for Extreme Environment Research (NEXER-Chile) NXR17-001 Versión publicada - versión final del editor Article in Journal/Newspaper Antarc* Antarctic Universidad de Chile: Repositorio académico Antarctic Fildes ENVELOPE(-58.817,-58.817,-62.217,-62.217) Fildes peninsula ENVELOPE(-58.948,-58.948,-62.182,-62.182) Inach ENVELOPE(-60.783,-60.783,-62.467,-62.467) Biology 10 10 1004 |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
ROS Mn oxidation Antarctic soil |
spellingShingle |
ROS Mn oxidation Antarctic soil Jofré, Ignacio Matus, Francisco Mendoza, Daniela Nájera De Ferrari, Francisco Merino, Carolina Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) release Reactive Oxygen Species (ROS) as secondary Mn(II) oxidation mechanisms to avoid toxicity |
topic_facet |
ROS Mn oxidation Antarctic soil |
description |
Manganese (Mn)-oxidizing bacteria (MnOxb) are an essential group of microorganisms that oxidize soluble Mn(II) to form precipitate Mn(III) minerals, playing a crucial role in soil formation. The Fildes Peninsula is one of the fastest-warming areas globally and, therefore, the maritime Antarctic soils from this pivotal location allow for the examination of the effect of temperature on bacterial communities. The temperature causes an increase in the microbial respiratory rate, producing reactive oxygen species (ROS), which are harmful to bacteria. We evaluate an evasive secondary non-enzymatic mechanism for ROS production under increasing temperature in MnOxb isolated from Antarctic soils. Bacteria produce ROS capable of oxidizing Mn(II) as temperature increases, contributing to the enzymatic pathway protecting microbial cells from Mn(II) toxicity. In addition, we determine that certain strains, such as Arthobacter oxydans, can use these ROS as mechanisms to protect themselves from Mn toxicity at high concentrations. In conclusion, we describe a secondary mechanism of Mn(II) oxidation in bacterial strains of Antarctic soils. Manganese (Mn) oxidation is performed through oxidative Mn-oxidizing bacteria (MnOxb) as the main bio-weathering mechanism for Mn(III/IV) deposits during soil formation. However, with an increase in temperature, the respiration rate also increases, producing Reactive Oxygen Species (ROS) as by-products, which are harmful to microbial cells. We hypothesize that bacterial ROS oxidize Mn(II) to Mn(III/IV) as a secondary non-enzymatic temperature-dependent mechanism for cell protection. Fourteen MnOxb were isolated from Antarctic soils under the global warming effect, and peroxidase (PO) activity, ROS, and Mn(III/IV) production were evaluated for 120 h of incubation at 4 degrees C, 15 degrees C, and 30 degrees C. ROS contributions to Mn oxidation were evaluated in Arthrobacter oxydans under antioxidant (Trolox) and ROS-stimulated (menadione) conditions. The Mn(III/IV) concentration increased with temperature and positively correlated with ROS production. ROS scavenging with Trolox depleted the Mn oxidation, and ROS-stimulant increased the Mn precipitation in A. oxydans. Increasing the Mn(II) concentration caused a reduction in the membrane potential and bacterial viability, which resulted in Mn precipitation on the bacteria surface. In conclusion, bacterial ROS production serves as a complementary non-enzymatic temperature-dependent mechanism for Mn(II) oxidation as a response in warming environments. INACH RT_23_17 ANID Fondecyt N3200758 Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT N 1180521 Network for Extreme Environment Research (NEXER-Chile) NXR17-001 Versión publicada - versión final del editor |
format |
Article in Journal/Newspaper |
author |
Jofré, Ignacio Matus, Francisco Mendoza, Daniela Nájera De Ferrari, Francisco Merino, Carolina |
author_facet |
Jofré, Ignacio Matus, Francisco Mendoza, Daniela Nájera De Ferrari, Francisco Merino, Carolina |
author_sort |
Jofré, Ignacio |
title |
Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) release Reactive Oxygen Species (ROS) as secondary Mn(II) oxidation mechanisms to avoid toxicity |
title_short |
Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) release Reactive Oxygen Species (ROS) as secondary Mn(II) oxidation mechanisms to avoid toxicity |
title_full |
Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) release Reactive Oxygen Species (ROS) as secondary Mn(II) oxidation mechanisms to avoid toxicity |
title_fullStr |
Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) release Reactive Oxygen Species (ROS) as secondary Mn(II) oxidation mechanisms to avoid toxicity |
title_full_unstemmed |
Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) release Reactive Oxygen Species (ROS) as secondary Mn(II) oxidation mechanisms to avoid toxicity |
title_sort |
manganese-oxidizing antarctic bacteria (mn-oxb) release reactive oxygen species (ros) as secondary mn(ii) oxidation mechanisms to avoid toxicity |
publisher |
MDPI |
publishDate |
2021 |
url |
https://doi.org/10.3390/biology10101004 https://repositorio.uchile.cl/handle/2250/184036 |
long_lat |
ENVELOPE(-58.817,-58.817,-62.217,-62.217) ENVELOPE(-58.948,-58.948,-62.182,-62.182) ENVELOPE(-60.783,-60.783,-62.467,-62.467) |
geographic |
Antarctic Fildes Fildes peninsula Inach |
geographic_facet |
Antarctic Fildes Fildes peninsula Inach |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Biology |
op_relation |
Biology 2021, 10, 1004 doi:10.3390/biology10101004 https://repositorio.uchile.cl/handle/2250/184036 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.3390/biology10101004 |
container_title |
Biology |
container_volume |
10 |
container_issue |
10 |
container_start_page |
1004 |
_version_ |
1766108688125263872 |