Antarctic thraustochytrids as sources of carotenoids and high-value fatty acids
Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and carotenoids are needed as human dietary supplements and are essential components in commercial feeds for the production of aquacultured seafood. Microorganisms such as thraustochytrids are potential natural sources of these compounds. This...
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Online Access: | https://doi.org/10.3390/md19070386 https://repositorio.uchile.cl/handle/2250/183020 |
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ftunivchile:oai:repositorio.uchile.cl:2250/183020 2023-05-15T13:38:36+02:00 Antarctic thraustochytrids as sources of carotenoids and high-value fatty acids Leyton, Allison Flores, Liset Shene de Vidts, Carolina María Chisti, Yusuf Larama, Giovanni Asenjo De Leuze, Juan Alfonso Armenta, Roberto E. 2021 application/pdf https://doi.org/10.3390/md19070386 https://repositorio.uchile.cl/handle/2250/183020 en eng MDPI Mar. Drugs 2021, 19, 386. doi:10.3390/md19070386 https://repositorio.uchile.cl/handle/2250/183020 Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ CC-BY-NC-ND Marine Drugs Thraustochytrium Antarctic thraustochytrids Microbial carotenoids Canthaxantin Docosahexaenoic acid Eicosapentaenoic acid Artículo de revista 2021 ftunivchile https://doi.org/10.3390/md19070386 2021-12-05T00:49:59Z Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and carotenoids are needed as human dietary supplements and are essential components in commercial feeds for the production of aquacultured seafood. Microorganisms such as thraustochytrids are potential natural sources of these compounds. This research reports on the lipid and carotenoid production capacity of thraustochytrids that were isolated from coastal waters of Antarctica. Of the 22 isolates, 21 produced lipids containing EPA+DHA, and the amount of these fatty acids exceeded 20% of the total fatty acids in 12 isolates. Ten isolates were shown to produce carotenoids (27.4–63.9 g/g dry biomass). The isolate RT2316-16, identified as Thraustochytrium sp., was the best producer of biomass (7.2 g/L in five days) rich in carotenoids (63.9 g/g) and, therefore, became the focus of this investigation. The main carotenoids in RT2316-16 were -carotene and canthaxanthin. The content of EPA+DHA in the total lipids (34 3% w/w in dry biomass) depended on the stage of growth of RT2316-16. Lipid and carotenoid content of the biomass and its concentration could be enhanced by modifying the composition of the culture medium. The estimated genome size of RT2316-16 was 44 Mb. Of the 5656 genes predicted from the genome, 4559 were annotated. These included genes of most of the enzymes in the elongation and desaturation pathway of synthesis of !-3 polyunsaturated fatty acids. Carotenoid precursors in RT2316-16 were synthesized through the mevalonate pathway. A -carotene synthase gene, with a different domain organization compared to the gene in other thraustochytrids, explained the carotenoid profile of RT2316-16. ANID Fondecyt 1200642 Centre for Biotechnology and Bioengineering (CeBiB) FB-0001 Versión publicada - versión final del editor Article in Journal/Newspaper Antarc* Antarctic Antarctica Universidad de Chile: Repositorio académico Antarctic Marine Drugs 19 7 386 |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
Thraustochytrium Antarctic thraustochytrids Microbial carotenoids Canthaxantin Docosahexaenoic acid Eicosapentaenoic acid |
spellingShingle |
Thraustochytrium Antarctic thraustochytrids Microbial carotenoids Canthaxantin Docosahexaenoic acid Eicosapentaenoic acid Leyton, Allison Flores, Liset Shene de Vidts, Carolina María Chisti, Yusuf Larama, Giovanni Asenjo De Leuze, Juan Alfonso Armenta, Roberto E. Antarctic thraustochytrids as sources of carotenoids and high-value fatty acids |
topic_facet |
Thraustochytrium Antarctic thraustochytrids Microbial carotenoids Canthaxantin Docosahexaenoic acid Eicosapentaenoic acid |
description |
Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and carotenoids are needed as human dietary supplements and are essential components in commercial feeds for the production of aquacultured seafood. Microorganisms such as thraustochytrids are potential natural sources of these compounds. This research reports on the lipid and carotenoid production capacity of thraustochytrids that were isolated from coastal waters of Antarctica. Of the 22 isolates, 21 produced lipids containing EPA+DHA, and the amount of these fatty acids exceeded 20% of the total fatty acids in 12 isolates. Ten isolates were shown to produce carotenoids (27.4–63.9 g/g dry biomass). The isolate RT2316-16, identified as Thraustochytrium sp., was the best producer of biomass (7.2 g/L in five days) rich in carotenoids (63.9 g/g) and, therefore, became the focus of this investigation. The main carotenoids in RT2316-16 were -carotene and canthaxanthin. The content of EPA+DHA in the total lipids (34 3% w/w in dry biomass) depended on the stage of growth of RT2316-16. Lipid and carotenoid content of the biomass and its concentration could be enhanced by modifying the composition of the culture medium. The estimated genome size of RT2316-16 was 44 Mb. Of the 5656 genes predicted from the genome, 4559 were annotated. These included genes of most of the enzymes in the elongation and desaturation pathway of synthesis of !-3 polyunsaturated fatty acids. Carotenoid precursors in RT2316-16 were synthesized through the mevalonate pathway. A -carotene synthase gene, with a different domain organization compared to the gene in other thraustochytrids, explained the carotenoid profile of RT2316-16. ANID Fondecyt 1200642 Centre for Biotechnology and Bioengineering (CeBiB) FB-0001 Versión publicada - versión final del editor |
format |
Article in Journal/Newspaper |
author |
Leyton, Allison Flores, Liset Shene de Vidts, Carolina María Chisti, Yusuf Larama, Giovanni Asenjo De Leuze, Juan Alfonso Armenta, Roberto E. |
author_facet |
Leyton, Allison Flores, Liset Shene de Vidts, Carolina María Chisti, Yusuf Larama, Giovanni Asenjo De Leuze, Juan Alfonso Armenta, Roberto E. |
author_sort |
Leyton, Allison |
title |
Antarctic thraustochytrids as sources of carotenoids and high-value fatty acids |
title_short |
Antarctic thraustochytrids as sources of carotenoids and high-value fatty acids |
title_full |
Antarctic thraustochytrids as sources of carotenoids and high-value fatty acids |
title_fullStr |
Antarctic thraustochytrids as sources of carotenoids and high-value fatty acids |
title_full_unstemmed |
Antarctic thraustochytrids as sources of carotenoids and high-value fatty acids |
title_sort |
antarctic thraustochytrids as sources of carotenoids and high-value fatty acids |
publisher |
MDPI |
publishDate |
2021 |
url |
https://doi.org/10.3390/md19070386 https://repositorio.uchile.cl/handle/2250/183020 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Marine Drugs |
op_relation |
Mar. Drugs 2021, 19, 386. doi:10.3390/md19070386 https://repositorio.uchile.cl/handle/2250/183020 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.3390/md19070386 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
7 |
container_start_page |
386 |
_version_ |
1766108659688931328 |