Characterization of the Gut microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii
Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding...
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ftunivchile:oai:repositorio.uchile.cl:2250/174743 2023-05-15T13:34:34+02:00 Characterization of the Gut microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii Schwob, Guillaume Cabrol, Lea Poulin, Elie Orlando, Julieta 2020 application/pdf https://doi.org/10.3389/fmicb.2020.00308 https://repositorio.uchile.cl/handle/2250/174743 en eng Frontiers Media Front. Microbiol. February 2020 %7C Volume 11 %7C Article 308 doi:10.3389/fmicb.2020.00308 https://repositorio.uchile.cl/handle/2250/174743 Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ CC-BY-NC-ND Frontiers in Microbiology Heart sea urchin Deposit-feeder Abatus agassizii Gut microbiota Core-microbiota Keystone West Antarctic Peninsula Antarctica Artículo de revista 2020 ftunivchile https://doi.org/10.3389/fmicb.2020.00308 2022-12-25T00:50:59Z Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding habit, it harbors a long and twice-looped digestive tract suggesting that it may host a complex bacterial community. Here, we characterized the gut microbiota of specimens from two A. agassizii populations at the south of the King George Island in the West Antarctic Peninsula. Using a metabarcoding approach targeting the 16S rRNA gene, we characterized the Abatus microbiota composition and putative functional capacity, evaluating its differentiation among the gut content and the gut tissue in comparison with the external sediment. Additionally, we aimed to define a core gut microbiota between A. agassizii populations to identify potential keystone bacterial taxa. Our results show that the diversity and the composition of the microbiota, at both genetic and predicted functional levels, were mostly driven by the sample type, and to a lesser extent by the population location. Specific bacterial taxa, belonging mostly to Planctomycetacia and Spirochaetia, were differently enriched in the gut content and the gut tissue, respectively. Predictive functional profiles revealed higher abundance of specific pathways, as the sulfur cycle in the gut content and the amino acid metabolism, in the gut tissue. Further, the definition of a core microbiota allowed to obtain evidence of specific localization of bacterial taxa and the identification of potential keystone taxa assigned to the Desulfobacula and Spirochaeta genera as potentially host selected. The ecological relevance of these keystone taxa in the host metabolism is discussed. Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT): PIA ACT 172065, ECOS SUD-CONICYT C16B03, INACH-RT09-12. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica King George Island Universidad de Chile: Repositorio académico Antarctic Antarctic Peninsula Inach ENVELOPE(-60.783,-60.783,-62.467,-62.467) King George Island The Antarctic Frontiers in Microbiology 11 |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
Heart sea urchin Deposit-feeder Abatus agassizii Gut microbiota Core-microbiota Keystone West Antarctic Peninsula Antarctica |
spellingShingle |
Heart sea urchin Deposit-feeder Abatus agassizii Gut microbiota Core-microbiota Keystone West Antarctic Peninsula Antarctica Schwob, Guillaume Cabrol, Lea Poulin, Elie Orlando, Julieta Characterization of the Gut microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
topic_facet |
Heart sea urchin Deposit-feeder Abatus agassizii Gut microbiota Core-microbiota Keystone West Antarctic Peninsula Antarctica |
description |
Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding habit, it harbors a long and twice-looped digestive tract suggesting that it may host a complex bacterial community. Here, we characterized the gut microbiota of specimens from two A. agassizii populations at the south of the King George Island in the West Antarctic Peninsula. Using a metabarcoding approach targeting the 16S rRNA gene, we characterized the Abatus microbiota composition and putative functional capacity, evaluating its differentiation among the gut content and the gut tissue in comparison with the external sediment. Additionally, we aimed to define a core gut microbiota between A. agassizii populations to identify potential keystone bacterial taxa. Our results show that the diversity and the composition of the microbiota, at both genetic and predicted functional levels, were mostly driven by the sample type, and to a lesser extent by the population location. Specific bacterial taxa, belonging mostly to Planctomycetacia and Spirochaetia, were differently enriched in the gut content and the gut tissue, respectively. Predictive functional profiles revealed higher abundance of specific pathways, as the sulfur cycle in the gut content and the amino acid metabolism, in the gut tissue. Further, the definition of a core microbiota allowed to obtain evidence of specific localization of bacterial taxa and the identification of potential keystone taxa assigned to the Desulfobacula and Spirochaeta genera as potentially host selected. The ecological relevance of these keystone taxa in the host metabolism is discussed. Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT): PIA ACT 172065, ECOS SUD-CONICYT C16B03, INACH-RT09-12. |
format |
Article in Journal/Newspaper |
author |
Schwob, Guillaume Cabrol, Lea Poulin, Elie Orlando, Julieta |
author_facet |
Schwob, Guillaume Cabrol, Lea Poulin, Elie Orlando, Julieta |
author_sort |
Schwob, Guillaume |
title |
Characterization of the Gut microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_short |
Characterization of the Gut microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_full |
Characterization of the Gut microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_fullStr |
Characterization of the Gut microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_full_unstemmed |
Characterization of the Gut microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_sort |
characterization of the gut microbiota of the antarctic heart urchin (spatangoida) abatus agassizii |
publisher |
Frontiers Media |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.00308 https://repositorio.uchile.cl/handle/2250/174743 |
long_lat |
ENVELOPE(-60.783,-60.783,-62.467,-62.467) |
geographic |
Antarctic Antarctic Peninsula Inach King George Island The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula Inach King George Island The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica King George Island |
op_source |
Frontiers in Microbiology |
op_relation |
Front. Microbiol. February 2020 %7C Volume 11 %7C Article 308 doi:10.3389/fmicb.2020.00308 https://repositorio.uchile.cl/handle/2250/174743 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.3389/fmicb.2020.00308 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
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1766054380071550976 |