Optimization of florfenicol dose against Piscirickettsia salmonis in Salmo salar through PK/PD studies
Salmonid Rickettsial Septicemia (SRS) is the disease of greatest economic importance in the Chilean salmon farming industry, causing high mortality in fish during the final stage of their productive cycle at sea. Since current, commercially available vaccines have not demonstrated the expected effic...
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ftunivchile:oai:repositorio.uchile.cl:2250/172544 2023-05-15T15:33:03+02:00 Optimization of florfenicol dose against Piscirickettsia salmonis in Salmo salar through PK/PD studies San Martín Núñez, Betty Fresno, Marcela Cornejo, Javiera Godoy, Marcos Ibarra, Rolando Vidal, Roberto Araneda, Marcelo Anadón, Arturo Lapierre Acevedo, Lisette 2019 application/pdf https://doi.org/10.1371/journal.pone.0215174 https://repositorio.uchile.cl/handle/2250/172544 en eng Public Library of Science PLoS ONE, Volumen 14, Issue 5, 2019, 19326203 doi:10.1371/journal.pone.0215174 https://repositorio.uchile.cl/handle/2250/172544 Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ CC-BY-NC-ND PLoS ONE Biochemistry Genetics and Molecular Biology (all) Agricultural and Biological Sciences (all) Artículo de revista 2019 ftunivchile https://doi.org/10.1371/journal.pone.0215174 2022-09-24T23:51:14Z Salmonid Rickettsial Septicemia (SRS) is the disease of greatest economic importance in the Chilean salmon farming industry, causing high mortality in fish during the final stage of their productive cycle at sea. Since current, commercially available vaccines have not demonstrated the expected efficacy levels, antimicrobials, most commonly florfenicol, are still the main resource for the treatment and control of this pathogen. The aim of this study was to determine the most appropriate single dose of florfenicol, administered through medicated feed, for the treatment of Piscirickettsia salmonis (P. salmonis), using pharmacokinetic/pharmacodynamic (PK/PD) models. Previously, Minimum Inhibitory Concentrations (MICs) of florfenicol were determined for 87 P. salmonis isolates in order to define the epidemiological cut-off point (COWT). The most commonly observed MIC was 0.125 μg mL-1 (83.7%). The COWT value was 0.25 μg mL-1 with a standard deviation of 0.47 log2 μg mL-1 and 0.36 log2 μg mL-1, for Normalized resistance interpretation (NRI) method and ECOFFinder method, respectively. A MIC of 1 μg mL-1 was considered the pharmacodynamic value (PD) to define PK/PD indices. Three doses of florfenicol were evaluated in fish farmed under controlled conditions. For each dose, 150 fish were used and blood plasma samples were collected at different time points (0–48 hours). PK parameters were obtained from curves representing plasma concentrations as a function of time. The results of Monte Carlo simulation indicate that at a dose of 20 mg/Kg l.w. of florfenicol, administered orally as medicated feed, there is 100% probability (PTA) of achieving the desired efficacy (AUC0-24h/MIC>125). According to these results, we suggest that at the indicated dose, the PK/PD cut-off point for florfenicol versus P. salmonis could be 2 μg mL-1 (PTA = 99%). In order to assess the indicated dose in Atlantic salmon, fish were inoculated with P. salmonis LF-89 strain and then treated with the optimized dose of florfenicol, 20 mg/Kg bw for 15 ... Article in Journal/Newspaper Atlantic salmon Salmo salar Universidad de Chile: Repositorio académico PLOS ONE 14 5 e0215174 |
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Universidad de Chile: Repositorio académico |
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ftunivchile |
language |
English |
topic |
Biochemistry Genetics and Molecular Biology (all) Agricultural and Biological Sciences (all) |
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Biochemistry Genetics and Molecular Biology (all) Agricultural and Biological Sciences (all) San Martín Núñez, Betty Fresno, Marcela Cornejo, Javiera Godoy, Marcos Ibarra, Rolando Vidal, Roberto Araneda, Marcelo Anadón, Arturo Lapierre Acevedo, Lisette Optimization of florfenicol dose against Piscirickettsia salmonis in Salmo salar through PK/PD studies |
topic_facet |
Biochemistry Genetics and Molecular Biology (all) Agricultural and Biological Sciences (all) |
description |
Salmonid Rickettsial Septicemia (SRS) is the disease of greatest economic importance in the Chilean salmon farming industry, causing high mortality in fish during the final stage of their productive cycle at sea. Since current, commercially available vaccines have not demonstrated the expected efficacy levels, antimicrobials, most commonly florfenicol, are still the main resource for the treatment and control of this pathogen. The aim of this study was to determine the most appropriate single dose of florfenicol, administered through medicated feed, for the treatment of Piscirickettsia salmonis (P. salmonis), using pharmacokinetic/pharmacodynamic (PK/PD) models. Previously, Minimum Inhibitory Concentrations (MICs) of florfenicol were determined for 87 P. salmonis isolates in order to define the epidemiological cut-off point (COWT). The most commonly observed MIC was 0.125 μg mL-1 (83.7%). The COWT value was 0.25 μg mL-1 with a standard deviation of 0.47 log2 μg mL-1 and 0.36 log2 μg mL-1, for Normalized resistance interpretation (NRI) method and ECOFFinder method, respectively. A MIC of 1 μg mL-1 was considered the pharmacodynamic value (PD) to define PK/PD indices. Three doses of florfenicol were evaluated in fish farmed under controlled conditions. For each dose, 150 fish were used and blood plasma samples were collected at different time points (0–48 hours). PK parameters were obtained from curves representing plasma concentrations as a function of time. The results of Monte Carlo simulation indicate that at a dose of 20 mg/Kg l.w. of florfenicol, administered orally as medicated feed, there is 100% probability (PTA) of achieving the desired efficacy (AUC0-24h/MIC>125). According to these results, we suggest that at the indicated dose, the PK/PD cut-off point for florfenicol versus P. salmonis could be 2 μg mL-1 (PTA = 99%). In order to assess the indicated dose in Atlantic salmon, fish were inoculated with P. salmonis LF-89 strain and then treated with the optimized dose of florfenicol, 20 mg/Kg bw for 15 ... |
format |
Article in Journal/Newspaper |
author |
San Martín Núñez, Betty Fresno, Marcela Cornejo, Javiera Godoy, Marcos Ibarra, Rolando Vidal, Roberto Araneda, Marcelo Anadón, Arturo Lapierre Acevedo, Lisette |
author_facet |
San Martín Núñez, Betty Fresno, Marcela Cornejo, Javiera Godoy, Marcos Ibarra, Rolando Vidal, Roberto Araneda, Marcelo Anadón, Arturo Lapierre Acevedo, Lisette |
author_sort |
San Martín Núñez, Betty |
title |
Optimization of florfenicol dose against Piscirickettsia salmonis in Salmo salar through PK/PD studies |
title_short |
Optimization of florfenicol dose against Piscirickettsia salmonis in Salmo salar through PK/PD studies |
title_full |
Optimization of florfenicol dose against Piscirickettsia salmonis in Salmo salar through PK/PD studies |
title_fullStr |
Optimization of florfenicol dose against Piscirickettsia salmonis in Salmo salar through PK/PD studies |
title_full_unstemmed |
Optimization of florfenicol dose against Piscirickettsia salmonis in Salmo salar through PK/PD studies |
title_sort |
optimization of florfenicol dose against piscirickettsia salmonis in salmo salar through pk/pd studies |
publisher |
Public Library of Science |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pone.0215174 https://repositorio.uchile.cl/handle/2250/172544 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
PLoS ONE |
op_relation |
PLoS ONE, Volumen 14, Issue 5, 2019, 19326203 doi:10.1371/journal.pone.0215174 https://repositorio.uchile.cl/handle/2250/172544 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1371/journal.pone.0215174 |
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PLOS ONE |
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14 |
container_issue |
5 |
container_start_page |
e0215174 |
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1766363527234191360 |