Pectinase secreted by psychrotolerant fungi: Identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.
Background: Pectinolytic enzymes, which are used in several industries, especially in the clarification process during wine and fruit juice production, represent approximately 10% of the global enzyme market. To prevent the proliferation of undesired microorganisms, to retain labile and volatile fla...
Published in: | Microbial Cell Factories |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BioMed Central Ltd.
2019
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12934-019-1092-2 https://repositorio.uchile.cl/handle/2250/171732 |
id |
ftunivchile:oai:repositorio.uchile.cl:2250/171732 |
---|---|
record_format |
openpolar |
spelling |
ftunivchile:oai:repositorio.uchile.cl:2250/171732 2023-05-15T14:01:29+02:00 Pectinase secreted by psychrotolerant fungi: Identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp. Carrasco, Mario Rozas, Juan Manuel Alcaíno Gorman, Jennifer Cifuentes, Víctor Baeza, Marcelo 2019 application/pdf https://doi.org/10.1186/s12934-019-1092-2 https://repositorio.uchile.cl/handle/2250/171732 en eng BioMed Central Ltd. Microbial Cell Factories, Volumen 18, Issue 1, 2019, 14752859 doi:10.1186/s12934-019-1092-2 https://repositorio.uchile.cl/handle/2250/171732 Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ CC-BY-NC-ND Microbial Cell Factories Biotechnology Bioengineering Applied Microbiology and Biotechnology Artículo de revista 2019 ftunivchile https://doi.org/10.1186/s12934-019-1092-2 2022-09-24T23:50:06Z Background: Pectinolytic enzymes, which are used in several industries, especially in the clarification process during wine and fruit juice production, represent approximately 10% of the global enzyme market. To prevent the proliferation of undesired microorganisms, to retain labile and volatile flavor compounds, and to save energy, the current trend is to perform this process at low temperatures. However, the commercially available pectinases are highly active at temperatures approximately 50 °C and poorly active at temperatures below 35 °C, which is the reason why there is a constant search for cold-active pectinases. In preliminary studies, pectinolytic activity was detected in cold-adapted yeasts and yeast-like microorganisms isolated from Antarctica. The aim of the present work was to characterize pectinases secreted by these microorganisms and to express the best candidate in Pichia pastoris. Results: Degradation of pectin by extracellular protein extracellu Article in Journal/Newspaper Antarc* Antarctica Universidad de Chile: Repositorio académico Microbial Cell Factories 18 1 |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
Biotechnology Bioengineering Applied Microbiology and Biotechnology |
spellingShingle |
Biotechnology Bioengineering Applied Microbiology and Biotechnology Carrasco, Mario Rozas, Juan Manuel Alcaíno Gorman, Jennifer Cifuentes, Víctor Baeza, Marcelo Pectinase secreted by psychrotolerant fungi: Identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp. |
topic_facet |
Biotechnology Bioengineering Applied Microbiology and Biotechnology |
description |
Background: Pectinolytic enzymes, which are used in several industries, especially in the clarification process during wine and fruit juice production, represent approximately 10% of the global enzyme market. To prevent the proliferation of undesired microorganisms, to retain labile and volatile flavor compounds, and to save energy, the current trend is to perform this process at low temperatures. However, the commercially available pectinases are highly active at temperatures approximately 50 °C and poorly active at temperatures below 35 °C, which is the reason why there is a constant search for cold-active pectinases. In preliminary studies, pectinolytic activity was detected in cold-adapted yeasts and yeast-like microorganisms isolated from Antarctica. The aim of the present work was to characterize pectinases secreted by these microorganisms and to express the best candidate in Pichia pastoris. Results: Degradation of pectin by extracellular protein extracellu |
format |
Article in Journal/Newspaper |
author |
Carrasco, Mario Rozas, Juan Manuel Alcaíno Gorman, Jennifer Cifuentes, Víctor Baeza, Marcelo |
author_facet |
Carrasco, Mario Rozas, Juan Manuel Alcaíno Gorman, Jennifer Cifuentes, Víctor Baeza, Marcelo |
author_sort |
Carrasco, Mario |
title |
Pectinase secreted by psychrotolerant fungi: Identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp. |
title_short |
Pectinase secreted by psychrotolerant fungi: Identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp. |
title_full |
Pectinase secreted by psychrotolerant fungi: Identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp. |
title_fullStr |
Pectinase secreted by psychrotolerant fungi: Identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp. |
title_full_unstemmed |
Pectinase secreted by psychrotolerant fungi: Identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp. |
title_sort |
pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from tetracladium sp. |
publisher |
BioMed Central Ltd. |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12934-019-1092-2 https://repositorio.uchile.cl/handle/2250/171732 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Microbial Cell Factories |
op_relation |
Microbial Cell Factories, Volumen 18, Issue 1, 2019, 14752859 doi:10.1186/s12934-019-1092-2 https://repositorio.uchile.cl/handle/2250/171732 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1186/s12934-019-1092-2 |
container_title |
Microbial Cell Factories |
container_volume |
18 |
container_issue |
1 |
_version_ |
1766271327667224576 |