Supramolecular hydrogels of β-cyclodextrin linked to calcium homopoly-L-guluronate for release of coumarins with trypanocidal activity

• Published in: Carbohydrate Polymers
• Main Authors: Moncada Basualto, Mauricio, Matsuhiro, B., Mansilla, A., Lapier, M., Maya Arango, Juan, Olea Azar, Claudio
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2019
• Subjects: 3-Amidocoumarins; Homopoly-L-guluronate; Inclusion complex; Sub-Antarctic macroalgae; Supramolecular hydrogel; Trypanosoma cruzi; Antarctic; Antarc*; Antarctica
• Online Access: https://doi.org/10.1016/j.carbpol.2018.10.010; https://repositorio.uchile.cl/handle/2250/171336

Association constants and thermodynamicsparameters on inclusion of four 3-amido coumarins that present trypanocidal activity, into 6-amino-beta-cyclodextrin (1: 1 stoichimetry) were determined. In addition, pure homopolymeric-a-L-guluronate fraction prepared by partial hydrolysis of sodium alginate from Sub-Antarctic Kelp Durvillaea antarctica was conjugated with 6-amino-beta-cyclodextrin (64% yield). To glycoconjugates, 3-amido coumarins were incorporated (73% of encapsulation) and supramolecular hydrogels were prepared by gelation with Ca2+ ions. The trypanocidal activity of the inclusion complexes increased by 10%. Likewise, an increase in diffusion in artificial membrane was observed (13%). It was found that the inclusion complexes increased the variation of the mitochondrial potential of T. cruzi (17%). The lowest release of substituted amidocoumarins (ACS) from supramolecular hydrogels occurred at pH 1.2 whereas the maximum release (34%) was observed at pH 8.0. Encapsulation of lipophilic bioactive compounds in supramolecular hydrogels allows the generation of release systems sensitive to pH with potential application in biomedicine.