Immunolocalization of morphogen sonic hedgehog in salmon fry (Salmo salar) Inmunolocalización del morfógeno sonic hedhehog en alevines de salmón (Salmo salar)
© 2016, Universidad de la Frontera. All rights reserved.With the purpose of carrying out a diagnosis of the different pathologies that affect the salmon fry stage (Salmo salar) and analyze the regeneration phases of the organizational centers and subjacent tissue in case of an amputation, we realize...
Published in: | International Journal of Morphology |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Universidad de la Frontera
2016
|
Subjects: | |
Online Access: | https://doi.org/10.4067/S0717-95022016000200054 https://repositorio.uchile.cl/handle/2250/166832 |
id |
ftunivchile:oai:repositorio.uchile.cl:2250/166832 |
---|---|
record_format |
openpolar |
spelling |
ftunivchile:oai:repositorio.uchile.cl:2250/166832 2023-05-15T18:09:37+02:00 Immunolocalization of morphogen sonic hedgehog in salmon fry (Salmo salar) Inmunolocalización del morfógeno sonic hedhehog en alevines de salmón (Salmo salar) Rojas, Mariana Saint-Pierre, Gustavo Hartley, Ricardo Vásquez, Bélgica Conei, Daniel del Sol, Mariano 2016 application/pdf https://doi.org/10.4067/S0717-95022016000200054 https://repositorio.uchile.cl/handle/2250/166832 en eng Universidad de la Frontera International Journal of Morphology, Volumen 34, Issue 2, 2018, Pages 770-774 07179502 07179367 doi:10.4067/S0717-95022016000200054 https://repositorio.uchile.cl/handle/2250/166832 Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ CC-BY-NC-ND International Journal of Morphology Antibody antiShh Immunohistochemistry Salmon fry Sonic hedgehog Artículo de revista 2016 ftunivchile https://doi.org/10.4067/S0717-95022016000200054 2022-09-24T23:50:02Z © 2016, Universidad de la Frontera. All rights reserved.With the purpose of carrying out a diagnosis of the different pathologies that affect the salmon fry stage (Salmo salar) and analyze the regeneration phases of the organizational centers and subjacent tissue in case of an amputation, we realized a study that allowed identifying the temporary and spatial location of the Sonic Hedgehog (Shh) morphogen in hatched fry stage. Fifteen salmon fry (Salmo salar) were used. They were anesthetized with 5 % benzocaine (BZ-20®, Veterquímica), fixed in 10 % buffered formalin, and embedded in paraffin, Shh polyclonal antibody (Santa Cruz H-160, rabbit) was used diluted at 1/100. They were subsequently rinsed in PBS-1 % Triton and incubated with anti-rabbit conjugated polymer antibody and HRP for 10-15 min. The development was done with DAB (Vector) for 1-5 min. The negative control was incubated without primary antibody. As an internal positive control the notochord was considered. Serial sagitt Article in Journal/Newspaper Salmo salar Universidad de Chile: Repositorio académico Triton ENVELOPE(-55.615,-55.615,49.517,49.517) International Journal of Morphology 34 2 770 774 |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
Antibody antiShh Immunohistochemistry Salmon fry Sonic hedgehog |
spellingShingle |
Antibody antiShh Immunohistochemistry Salmon fry Sonic hedgehog Rojas, Mariana Saint-Pierre, Gustavo Hartley, Ricardo Vásquez, Bélgica Conei, Daniel del Sol, Mariano Immunolocalization of morphogen sonic hedgehog in salmon fry (Salmo salar) Inmunolocalización del morfógeno sonic hedhehog en alevines de salmón (Salmo salar) |
topic_facet |
Antibody antiShh Immunohistochemistry Salmon fry Sonic hedgehog |
description |
© 2016, Universidad de la Frontera. All rights reserved.With the purpose of carrying out a diagnosis of the different pathologies that affect the salmon fry stage (Salmo salar) and analyze the regeneration phases of the organizational centers and subjacent tissue in case of an amputation, we realized a study that allowed identifying the temporary and spatial location of the Sonic Hedgehog (Shh) morphogen in hatched fry stage. Fifteen salmon fry (Salmo salar) were used. They were anesthetized with 5 % benzocaine (BZ-20®, Veterquímica), fixed in 10 % buffered formalin, and embedded in paraffin, Shh polyclonal antibody (Santa Cruz H-160, rabbit) was used diluted at 1/100. They were subsequently rinsed in PBS-1 % Triton and incubated with anti-rabbit conjugated polymer antibody and HRP for 10-15 min. The development was done with DAB (Vector) for 1-5 min. The negative control was incubated without primary antibody. As an internal positive control the notochord was considered. Serial sagitt |
format |
Article in Journal/Newspaper |
author |
Rojas, Mariana Saint-Pierre, Gustavo Hartley, Ricardo Vásquez, Bélgica Conei, Daniel del Sol, Mariano |
author_facet |
Rojas, Mariana Saint-Pierre, Gustavo Hartley, Ricardo Vásquez, Bélgica Conei, Daniel del Sol, Mariano |
author_sort |
Rojas, Mariana |
title |
Immunolocalization of morphogen sonic hedgehog in salmon fry (Salmo salar) Inmunolocalización del morfógeno sonic hedhehog en alevines de salmón (Salmo salar) |
title_short |
Immunolocalization of morphogen sonic hedgehog in salmon fry (Salmo salar) Inmunolocalización del morfógeno sonic hedhehog en alevines de salmón (Salmo salar) |
title_full |
Immunolocalization of morphogen sonic hedgehog in salmon fry (Salmo salar) Inmunolocalización del morfógeno sonic hedhehog en alevines de salmón (Salmo salar) |
title_fullStr |
Immunolocalization of morphogen sonic hedgehog in salmon fry (Salmo salar) Inmunolocalización del morfógeno sonic hedhehog en alevines de salmón (Salmo salar) |
title_full_unstemmed |
Immunolocalization of morphogen sonic hedgehog in salmon fry (Salmo salar) Inmunolocalización del morfógeno sonic hedhehog en alevines de salmón (Salmo salar) |
title_sort |
immunolocalization of morphogen sonic hedgehog in salmon fry (salmo salar) inmunolocalización del morfógeno sonic hedhehog en alevines de salmón (salmo salar) |
publisher |
Universidad de la Frontera |
publishDate |
2016 |
url |
https://doi.org/10.4067/S0717-95022016000200054 https://repositorio.uchile.cl/handle/2250/166832 |
long_lat |
ENVELOPE(-55.615,-55.615,49.517,49.517) |
geographic |
Triton |
geographic_facet |
Triton |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
International Journal of Morphology |
op_relation |
International Journal of Morphology, Volumen 34, Issue 2, 2018, Pages 770-774 07179502 07179367 doi:10.4067/S0717-95022016000200054 https://repositorio.uchile.cl/handle/2250/166832 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.4067/S0717-95022016000200054 |
container_title |
International Journal of Morphology |
container_volume |
34 |
container_issue |
2 |
container_start_page |
770 |
op_container_end_page |
774 |
_version_ |
1766182223297380352 |