Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites
Microbiota presumably plays an essential role in inhibiting pathogen colonization and in the maintenance of health in oysters, but limited data exist concerning their different growth phases and conditions. We analyzed the bacterial microbiota composition of two commercial oysters: Crassostrea gigas...
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ftunivchile:oai:repositorio.uchile.cl:2250/165642 2023-05-15T15:57:48+02:00 Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites Trabal, Natalia Mazón Suástegui, José M. Vázquez Juárez, Ricardo Asencio-Valle, Felipe Morales-Bojórquez, Enrique Romero Ormazábal, Jaime 2012 application/pdf https://doi.org/10.1007/s00248-012-0039-5 https://repositorio.uchile.cl/handle/2250/165642 en eng Microbial Ecology, Volumen 64, Issue 2, 2018, Pages 555-569 00953628 doi:10.1007/s00248-012-0039-5 https://repositorio.uchile.cl/handle/2250/165642 Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ CC-BY-NC-ND Microbial Ecology Ecology Evolution Behavior and Systematics Soil Science Artículo de revista 2012 ftunivchile https://doi.org/10.1007/s00248-012-0039-5 2023-01-22T00:58:18Z Microbiota presumably plays an essential role in inhibiting pathogen colonization and in the maintenance of health in oysters, but limited data exist concerning their different growth phases and conditions. We analyzed the bacterial microbiota composition of two commercial oysters: Crassostrea gigas and Crassostrea corteziensis. Differences in microbiota were assayed in three growth phases: post-larvae at the hatchery, juvenile, and adult at two grow-out cultivation sites. Variations in the microbiota were assessed by PCR analysis of the 16S rRNA gene in DNA extracted from depurated oysters. Restriction fragment length polymorphism (RFLP) profiles were studied using Dice's similarity coefficient (Cs) and statistical principal component analysis (PCA). The microbiota composition was determined by sequencing temperature gradient gel electrophoresis (TGGE) bands. The RFLP analysis of post-larvae revealed homology in the microbiota of both oyster species (Cs > 88 %). Dice and PCA analyses Article in Journal/Newspaper Crassostrea gigas Universidad de Chile: Repositorio académico Microbial Ecology 64 2 555 569 |
institution |
Open Polar |
collection |
Universidad de Chile: Repositorio académico |
op_collection_id |
ftunivchile |
language |
English |
topic |
Ecology Evolution Behavior and Systematics Soil Science |
spellingShingle |
Ecology Evolution Behavior and Systematics Soil Science Trabal, Natalia Mazón Suástegui, José M. Vázquez Juárez, Ricardo Asencio-Valle, Felipe Morales-Bojórquez, Enrique Romero Ormazábal, Jaime Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites |
topic_facet |
Ecology Evolution Behavior and Systematics Soil Science |
description |
Microbiota presumably plays an essential role in inhibiting pathogen colonization and in the maintenance of health in oysters, but limited data exist concerning their different growth phases and conditions. We analyzed the bacterial microbiota composition of two commercial oysters: Crassostrea gigas and Crassostrea corteziensis. Differences in microbiota were assayed in three growth phases: post-larvae at the hatchery, juvenile, and adult at two grow-out cultivation sites. Variations in the microbiota were assessed by PCR analysis of the 16S rRNA gene in DNA extracted from depurated oysters. Restriction fragment length polymorphism (RFLP) profiles were studied using Dice's similarity coefficient (Cs) and statistical principal component analysis (PCA). The microbiota composition was determined by sequencing temperature gradient gel electrophoresis (TGGE) bands. The RFLP analysis of post-larvae revealed homology in the microbiota of both oyster species (Cs > 88 %). Dice and PCA analyses |
format |
Article in Journal/Newspaper |
author |
Trabal, Natalia Mazón Suástegui, José M. Vázquez Juárez, Ricardo Asencio-Valle, Felipe Morales-Bojórquez, Enrique Romero Ormazábal, Jaime |
author_facet |
Trabal, Natalia Mazón Suástegui, José M. Vázquez Juárez, Ricardo Asencio-Valle, Felipe Morales-Bojórquez, Enrique Romero Ormazábal, Jaime |
author_sort |
Trabal, Natalia |
title |
Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites |
title_short |
Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites |
title_full |
Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites |
title_fullStr |
Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites |
title_full_unstemmed |
Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites |
title_sort |
molecular analysis of bacterial microbiota associated with oysters (crassostrea gigas and crassostrea corteziensis) in different growth phases at two cultivation sites |
publishDate |
2012 |
url |
https://doi.org/10.1007/s00248-012-0039-5 https://repositorio.uchile.cl/handle/2250/165642 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Microbial Ecology |
op_relation |
Microbial Ecology, Volumen 64, Issue 2, 2018, Pages 555-569 00953628 doi:10.1007/s00248-012-0039-5 https://repositorio.uchile.cl/handle/2250/165642 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1007/s00248-012-0039-5 |
container_title |
Microbial Ecology |
container_volume |
64 |
container_issue |
2 |
container_start_page |
555 |
op_container_end_page |
569 |
_version_ |
1766393498930511872 |